Beihai sobemo-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE56|A0A1L3KE56_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 15 OX=1922686 PE=4 SV=1
MM1 pKa = 7.48SFNGVLPNQFHH12 pKa = 6.8LEE14 pKa = 4.09EE15 pKa = 4.81HH16 pKa = 6.73LSPYY20 pKa = 10.29TNYY23 pKa = 10.11QGSAPPMDD31 pKa = 4.22FHH33 pKa = 7.08PLGIKK38 pKa = 9.44TIEE41 pKa = 4.08LVHH44 pKa = 7.08ILLASLILLTMGLMCALLRR63 pKa = 11.84DD64 pKa = 4.41WFATTPLRR72 pKa = 11.84DD73 pKa = 3.24LLSRR77 pKa = 11.84KK78 pKa = 9.17RR79 pKa = 11.84RR80 pKa = 11.84PKK82 pKa = 10.72HH83 pKa = 5.86NIPDD87 pKa = 3.5ATEE90 pKa = 4.37DD91 pKa = 3.76EE92 pKa = 4.94EE93 pKa = 5.01EE94 pKa = 4.11EE95 pKa = 4.16QPLVVDD101 pKa = 4.44EE102 pKa = 4.89CPKK105 pKa = 10.42CRR107 pKa = 11.84PQQSPQPEE115 pKa = 4.4SNDD118 pKa = 3.21YY119 pKa = 8.2FTKK122 pKa = 10.67CKK124 pKa = 10.28LPKK127 pKa = 9.75EE128 pKa = 4.05QVILWRR134 pKa = 11.84TCCKK138 pKa = 10.37SDD140 pKa = 5.39DD141 pKa = 4.01PAKK144 pKa = 10.54PPLTTHH150 pKa = 6.3VGQAFFIAKK159 pKa = 7.85DD160 pKa = 3.62TLLTTAHH167 pKa = 6.57SIKK170 pKa = 10.44PGEE173 pKa = 4.35KK174 pKa = 9.45YY175 pKa = 10.78VISKK179 pKa = 9.45SQIGRR184 pKa = 11.84AFPVLSVQYY193 pKa = 7.98PTGPAKK199 pKa = 10.46DD200 pKa = 3.67VIYY203 pKa = 10.74LKK205 pKa = 10.73VEE207 pKa = 3.75NRR209 pKa = 11.84AFTQLEE215 pKa = 3.89ASVAKK220 pKa = 10.13IASEE224 pKa = 4.07YY225 pKa = 11.0LPGDD229 pKa = 3.75TVSVCGGGLEE239 pKa = 4.18PQEE242 pKa = 4.46SSDD245 pKa = 4.78RR246 pKa = 11.84ALQSIGNLEE255 pKa = 4.11PWEE258 pKa = 4.37GAAGILISAHH268 pKa = 5.74NCNTLKK274 pKa = 10.66GFSGAPIFCGNKK286 pKa = 8.26VVAMHH291 pKa = 6.32NQTQTDD297 pKa = 3.78MFNIATNLWPVLGTKK312 pKa = 10.05CQISPQFEE320 pKa = 4.38SSEE323 pKa = 3.66MDD325 pKa = 3.25ANFFRR330 pKa = 11.84HH331 pKa = 6.92DD332 pKa = 3.47DD333 pKa = 3.77TRR335 pKa = 11.84ILEE338 pKa = 4.42YY339 pKa = 11.03DD340 pKa = 3.44DD341 pKa = 3.58MVYY344 pKa = 10.71VSKK347 pKa = 11.02AGRR350 pKa = 11.84FYY352 pKa = 11.1NIDD355 pKa = 3.26RR356 pKa = 11.84QVYY359 pKa = 9.14QKK361 pKa = 10.93LKK363 pKa = 10.48QKK365 pKa = 9.78MPNTTDD371 pKa = 3.03SLYY374 pKa = 11.02VPPKK378 pKa = 8.88EE379 pKa = 4.28NAWADD384 pKa = 3.34GGEE387 pKa = 4.22EE388 pKa = 3.94IDD390 pKa = 4.47FNSPLVFEE398 pKa = 5.2SSNALPTPLTPLNVDD413 pKa = 3.76AVRR416 pKa = 11.84QANVAITQVNNAYY429 pKa = 9.88FALRR433 pKa = 11.84TMAMTTSRR441 pKa = 11.84LLNLALSQKK450 pKa = 10.54DD451 pKa = 3.53EE452 pKa = 4.44EE453 pKa = 4.83GAKK456 pKa = 10.36SLANNFTYY464 pKa = 10.53LSKK467 pKa = 10.86QLAPEE472 pKa = 4.25SFKK475 pKa = 10.26QTIRR479 pKa = 11.84DD480 pKa = 3.95AIDD483 pKa = 3.29NFSYY487 pKa = 9.9ATMLSEE493 pKa = 5.02QDD495 pKa = 3.02EE496 pKa = 4.47KK497 pKa = 11.35LAKK500 pKa = 9.36KK501 pKa = 8.48WKK503 pKa = 8.78KK504 pKa = 10.22RR505 pKa = 11.84RR506 pKa = 11.84DD507 pKa = 3.54AGDD510 pKa = 3.71PDD512 pKa = 5.23AEE514 pKa = 4.52FKK516 pKa = 11.01QITDD520 pKa = 3.9GYY522 pKa = 8.99IAEE525 pKa = 4.45IDD527 pKa = 3.89TYY529 pKa = 11.34SKK531 pKa = 11.31ALAGIPKK538 pKa = 10.06RR539 pKa = 11.84LNKK542 pKa = 9.91NDD544 pKa = 4.57LKK546 pKa = 10.83IQVPPEE552 pKa = 3.65PEE554 pKa = 3.76FNDD557 pKa = 3.55RR558 pKa = 11.84EE559 pKa = 4.01NAFFLKK565 pKa = 10.73YY566 pKa = 10.41GINFANPEE574 pKa = 4.42EE575 pKa = 4.34ILKK578 pKa = 10.8APAIEE583 pKa = 4.3LPKK586 pKa = 10.43KK587 pKa = 10.18PEE589 pKa = 3.67EE590 pKa = 4.26SEE592 pKa = 4.18PKK594 pKa = 10.58VILAPQRR601 pKa = 11.84PTQEE605 pKa = 3.84IRR607 pKa = 11.84NKK609 pKa = 5.46TTKK612 pKa = 10.48GKK614 pKa = 10.36LSKK617 pKa = 9.67TPNTSSVQVLNTPSAPSKK635 pKa = 10.59EE636 pKa = 4.19MEE638 pKa = 4.62AKK640 pKa = 10.49LVDD643 pKa = 3.77HH644 pKa = 6.3EE645 pKa = 5.0EE646 pKa = 4.13KK647 pKa = 10.86LKK649 pKa = 10.81SAKK652 pKa = 9.15QSKK655 pKa = 9.38LQEE658 pKa = 4.31EE659 pKa = 4.89CSGNCLTAQTGLEE672 pKa = 4.02SSIAQLTSLIQLLTTTIAASQLTSPPQTPSSKK704 pKa = 10.49LSPTTQKK711 pKa = 10.6KK712 pKa = 7.23PQGMSSLQAQPTQKK726 pKa = 10.11QSQLVSEE733 pKa = 4.86CTLSTIDD740 pKa = 4.98EE741 pKa = 4.21PRR743 pKa = 11.84KK744 pKa = 9.78DD745 pKa = 4.23LKK747 pKa = 11.26SLMQLQSEE755 pKa = 5.08GCRR758 pKa = 11.84RR759 pKa = 11.84DD760 pKa = 3.32MLSNLLRR767 pKa = 11.84PGVQCIKK774 pKa = 9.81ITGEE778 pKa = 4.18TIEE781 pKa = 4.73PCQDD785 pKa = 3.2SKK787 pKa = 11.71GSEE790 pKa = 3.95PSSLKK795 pKa = 8.21TTTDD799 pKa = 2.98AMCC802 pKa = 4.7

Molecular weight: 89.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE56|A0A1L3KE56_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 15 OX=1922686 PE=4 SV=1
MM1 pKa = 7.49SGLEE5 pKa = 3.93GFRR8 pKa = 11.84AFISEE13 pKa = 4.12NHH15 pKa = 7.0DD16 pKa = 3.09GRR18 pKa = 11.84HH19 pKa = 4.45VLEE22 pKa = 4.45PTSTPGYY29 pKa = 9.52PWVQLGNSNAEE40 pKa = 4.14CFGYY44 pKa = 10.75VPGLGYY50 pKa = 11.0VGDD53 pKa = 4.28KK54 pKa = 11.01VADD57 pKa = 4.51LYY59 pKa = 11.04QAVRR63 pKa = 11.84QRR65 pKa = 11.84LYY67 pKa = 10.62TLEE70 pKa = 4.18TTPKK74 pKa = 9.17IDD76 pKa = 4.95NIFLFIKK83 pKa = 10.29QEE85 pKa = 3.75PHH87 pKa = 6.53KK88 pKa = 9.82PAKK91 pKa = 9.42AQQRR95 pKa = 11.84RR96 pKa = 11.84WRR98 pKa = 11.84LISGIGATDD107 pKa = 3.36QLIAEE112 pKa = 4.29MLFRR116 pKa = 11.84PFLDD120 pKa = 2.92EE121 pKa = 4.0MTNNPCRR128 pKa = 11.84YY129 pKa = 9.46GMAVGWAPVLEE140 pKa = 4.12NAIPFIMSHH149 pKa = 6.47LSGLTVCADD158 pKa = 3.27KK159 pKa = 11.25SAWDD163 pKa = 3.46WTVQPWLCDD172 pKa = 3.41YY173 pKa = 8.59MKK175 pKa = 11.0SFLSKK180 pKa = 10.43LCPGVSTVVQNHH192 pKa = 5.03VEE194 pKa = 3.72AVMGKK199 pKa = 8.06KK200 pKa = 8.08TFQWPGGKK208 pKa = 8.45YY209 pKa = 8.81HH210 pKa = 6.47WDD212 pKa = 3.14KK213 pKa = 11.46RR214 pKa = 11.84GVFCSGWKK222 pKa = 8.38LTITGNSIMQHH233 pKa = 5.38ACHH236 pKa = 6.52RR237 pKa = 11.84MAGGTGNMQANGDD250 pKa = 3.97DD251 pKa = 3.71TCQAEE256 pKa = 4.57EE257 pKa = 4.18PPEE260 pKa = 3.77YY261 pKa = 10.05WEE263 pKa = 4.28NLRR266 pKa = 11.84KK267 pKa = 9.95LGPIIKK273 pKa = 9.74EE274 pKa = 3.54IRR276 pKa = 11.84RR277 pKa = 11.84DD278 pKa = 3.58GTIEE282 pKa = 3.94FCGLEE287 pKa = 3.95ITPDD291 pKa = 3.46GYY293 pKa = 11.69YY294 pKa = 10.29PMYY297 pKa = 9.82GAKK300 pKa = 8.37HH301 pKa = 5.23TFVLTHH307 pKa = 6.91LKK309 pKa = 10.24PKK311 pKa = 10.04IARR314 pKa = 11.84EE315 pKa = 3.97VLLSYY320 pKa = 10.61QYY322 pKa = 11.12LYY324 pKa = 10.42PFEE327 pKa = 4.54PEE329 pKa = 3.62KK330 pKa = 10.87LAAIQAWIASLGFEE344 pKa = 4.88KK345 pKa = 10.77DD346 pKa = 3.28LTPPEE351 pKa = 4.26VLRR354 pKa = 11.84GVPLGVII361 pKa = 4.07

Molecular weight: 40.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1163

361

802

581.5

64.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.137 ± 0.265

2.236 ± 0.14

4.815 ± 0.358

6.707 ± 0.332

3.611 ± 0.295

5.847 ± 1.788

1.806 ± 0.523

5.245 ± 0.16

7.051 ± 0.82

9.63 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.347

4.299 ± 0.529

7.309 ± 0.208

5.503 ± 0.731

3.783 ± 0.352

6.879 ± 1.478

6.793 ± 0.68

4.643 ± 0.637

1.462 ± 0.86

2.837 ± 0.715
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski