Microbacterium phage Araxxi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Burrovirus; Microbacterium virus Araxxi

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KT73|A0A516KT73_9CAUD Uncharacterized protein OS=Microbacterium phage Araxxi OX=2590948 GN=41 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 3.06INEE6 pKa = 4.28NTDD9 pKa = 3.52GLLTEE14 pKa = 4.69QDD16 pKa = 3.68QADD19 pKa = 4.34LAQLAAEE26 pKa = 4.17PLEE29 pKa = 4.15ITVLEE34 pKa = 4.24TWEE37 pKa = 4.4SVLSNIEE44 pKa = 3.57QMEE47 pKa = 4.11NEE49 pKa = 4.45KK50 pKa = 10.35IEE52 pKa = 3.83PSYY55 pKa = 11.28AVNIVGKK62 pKa = 9.08WPKK65 pKa = 10.2LDD67 pKa = 3.96YY68 pKa = 11.36GDD70 pKa = 4.15VPHH73 pKa = 7.27FYY75 pKa = 11.23KK76 pKa = 10.98LFNEE80 pKa = 3.89YY81 pKa = 10.25LKK83 pKa = 10.41RR84 pKa = 11.84YY85 pKa = 9.0RR86 pKa = 11.84AVLEE90 pKa = 4.1EE91 pKa = 3.86QLRR94 pKa = 11.84LHH96 pKa = 7.31PDD98 pKa = 2.96AKK100 pKa = 11.13DD101 pKa = 3.28NTGEE105 pKa = 4.12FGTEE109 pKa = 3.62EE110 pKa = 3.88SDD112 pKa = 4.43AIANRR117 pKa = 11.84DD118 pKa = 3.18IYY120 pKa = 11.03VEE122 pKa = 5.8LMFGWNVVTAEE133 pKa = 5.4LEE135 pKa = 4.46HH136 pKa = 6.67EE137 pKa = 4.06WDD139 pKa = 4.14ALDD142 pKa = 3.33VDD144 pKa = 4.23AAEE147 pKa = 5.4KK148 pKa = 9.5IAAMGEE154 pKa = 3.95AQLFVTGPQGMLQALTAPNVGFQWTNEE181 pKa = 3.84EE182 pKa = 4.11QIALTEE188 pKa = 4.11RR189 pKa = 11.84VVAAAEE195 pKa = 4.04EE196 pKa = 4.28LL197 pKa = 3.84

Molecular weight: 22.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KT34|A0A516KT34_9CAUD Uncharacterized protein OS=Microbacterium phage Araxxi OX=2590948 GN=26 PE=4 SV=1
MM1 pKa = 6.84NQEE4 pKa = 4.4LKK6 pKa = 11.08DD7 pKa = 3.7PFAGSRR13 pKa = 11.84LPSDD17 pKa = 3.78AAAPPQPEE25 pKa = 4.74RR26 pKa = 11.84YY27 pKa = 9.42DD28 pKa = 3.75QLFLPNDD35 pKa = 3.68EE36 pKa = 4.65KK37 pKa = 11.27PRR39 pKa = 11.84MPLTKK44 pKa = 9.86DD45 pKa = 2.8RR46 pKa = 11.84YY47 pKa = 8.12VVRR50 pKa = 11.84DD51 pKa = 3.83NPHH54 pKa = 4.81EE55 pKa = 4.32VAWEE59 pKa = 4.06RR60 pKa = 11.84QVRR63 pKa = 11.84MYY65 pKa = 11.16LRR67 pKa = 11.84GLSLDD72 pKa = 3.71DD73 pKa = 3.54SHH75 pKa = 6.38RR76 pKa = 11.84TSATMIYY83 pKa = 9.85EE84 pKa = 4.04WATGKK89 pKa = 10.28RR90 pKa = 11.84VADD93 pKa = 3.89QIAAGVATSPDD104 pKa = 3.09LRR106 pKa = 11.84KK107 pKa = 9.6INKK110 pKa = 8.92IMRR113 pKa = 11.84FYY115 pKa = 10.75FGKK118 pKa = 9.85PYY120 pKa = 8.65QTWIAGRR127 pKa = 11.84KK128 pKa = 8.74IPNCYY133 pKa = 9.9RR134 pKa = 11.84IPKK137 pKa = 7.65GWRR140 pKa = 11.84VKK142 pKa = 8.85RR143 pKa = 11.84HH144 pKa = 5.56RR145 pKa = 11.84PMTLTLWSEE154 pKa = 4.26YY155 pKa = 10.79TEE157 pKa = 4.22GTLNDD162 pKa = 3.62DD163 pKa = 3.73GARR166 pKa = 11.84RR167 pKa = 11.84NAAQQ171 pKa = 3.38

Molecular weight: 19.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

16885

43

4494

337.7

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.104 ± 0.534

0.444 ± 0.166

6.574 ± 0.227

5.988 ± 0.343

3.311 ± 0.141

7.77 ± 0.446

1.617 ± 0.223

4.625 ± 0.184

4.246 ± 0.362

8.22 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.168

4.661 ± 0.279

4.904 ± 0.2

4.762 ± 0.199

5.964 ± 0.321

6.189 ± 0.275

6.574 ± 0.338

6.532 ± 0.241

1.836 ± 0.127

3.092 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski