Pseudopedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643) (Pedobacter saltans)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pseudopedobacter; Pseudopedobacter saltans

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0S653|F0S653_PSESL DUF3347 domain-containing protein OS=Pseudopedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643) OX=762903 GN=Pedsa_1597 PE=4 SV=1
MM1 pKa = 7.7ANWCNNTIVFEE12 pKa = 4.41GTDD15 pKa = 3.4EE16 pKa = 5.06AIEE19 pKa = 4.69QITQLFKK26 pKa = 10.93TMAEE30 pKa = 4.03RR31 pKa = 11.84EE32 pKa = 4.18QKK34 pKa = 9.95EE35 pKa = 4.12NCGQLPEE42 pKa = 4.69FVQDD46 pKa = 3.45TNGDD50 pKa = 3.54YY51 pKa = 10.56FYY53 pKa = 11.09NISQDD58 pKa = 3.45NEE60 pKa = 4.35SFGVFQYY67 pKa = 7.18EE68 pKa = 4.85TKK70 pKa = 10.15WSPNTEE76 pKa = 3.8AVKK79 pKa = 10.62QIAEE83 pKa = 4.07HH84 pKa = 5.9FKK86 pKa = 11.12VNFTQDD92 pKa = 3.63YY93 pKa = 9.93EE94 pKa = 4.3EE95 pKa = 5.16LGCLVYY101 pKa = 10.63GQAILDD107 pKa = 4.73DD108 pKa = 4.08GTLTNTCLDD117 pKa = 3.64SQNFDD122 pKa = 3.2SYY124 pKa = 11.78EE125 pKa = 3.94LDD127 pKa = 3.77KK128 pKa = 11.24EE129 pKa = 4.07TDD131 pKa = 3.25TYY133 pKa = 11.17HH134 pKa = 7.45FEE136 pKa = 3.75GTEE139 pKa = 4.0YY140 pKa = 11.02DD141 pKa = 4.09SEE143 pKa = 4.16WDD145 pKa = 3.2ILEE148 pKa = 4.24TLLEE152 pKa = 4.21RR153 pKa = 11.84KK154 pKa = 9.36IEE156 pKa = 4.09SHH158 pKa = 6.47FNSSKK163 pKa = 9.98VV164 pKa = 3.24

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0S590|F0S590_PSESL Uncharacterized protein OS=Pseudopedobacter saltans (strain ATCC 51119 / DSM 12145 / JCM 21818 / LMG 10337 / NBRC 100064 / NCIMB 13643) OX=762903 GN=Pedsa_1474 PE=4 SV=1
MM1 pKa = 7.32NHH3 pKa = 6.34LQLAFIIGLFGSLHH17 pKa = 5.74CVGMCGPLAFAIPNTGSSRR36 pKa = 11.84WRR38 pKa = 11.84LVLNKK43 pKa = 9.83FIYY46 pKa = 10.07QIGRR50 pKa = 11.84AFSYY54 pKa = 10.81AILGFLIGIIGKK66 pKa = 8.98KK67 pKa = 9.55LWIAGFQQTLSVLCGAFIVVYY88 pKa = 9.93SIIRR92 pKa = 11.84LIPLRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 9.47FNSNFSIINDD109 pKa = 4.61WISKK113 pKa = 9.84AIQKK117 pKa = 10.2RR118 pKa = 11.84YY119 pKa = 8.14GHH121 pKa = 6.0FVVGLLNGILPCAFVYY137 pKa = 10.51VALATAVNTTSPVQSALFMFFFGLGTLPLMFSAAVGVSFAGHH179 pKa = 5.62NLRR182 pKa = 11.84RR183 pKa = 11.84SINNILPILSLLLGIWFILRR203 pKa = 11.84GSSLDD208 pKa = 3.52IPFLSPVLTGDD219 pKa = 4.09SAICRR224 pKa = 3.83

Molecular weight: 24.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3780

0

3780

1370524

30

6409

362.6

40.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.039

0.752 ± 0.013

5.41 ± 0.03

6.114 ± 0.039

5.051 ± 0.03

6.734 ± 0.037

1.71 ± 0.02

7.809 ± 0.038

7.824 ± 0.036

9.292 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.018

6.211 ± 0.045

3.512 ± 0.021

3.407 ± 0.022

3.73 ± 0.026

6.665 ± 0.036

5.464 ± 0.048

6.172 ± 0.029

1.159 ± 0.015

4.279 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski