SAR86 cluster bacterium SAR86E

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; SAR86 cluster

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1403 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6GJL4|K6GJL4_9GAMM ATP-dependent Clp protease ATP-binding subunit ClpX OS=SAR86 cluster bacterium SAR86E OX=1208365 GN=clpX PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.55NLLTLSLGLVFIASCGQTTTSTDD25 pKa = 2.95EE26 pKa = 3.88NVAYY30 pKa = 10.35DD31 pKa = 3.54LVGVQFYY38 pKa = 11.43NEE40 pKa = 5.39FIPCVPGSDD49 pKa = 3.45FTQEE53 pKa = 4.34NVDD56 pKa = 4.05SMMQGWRR63 pKa = 11.84SLNISDD69 pKa = 5.42DD70 pKa = 4.1LLGAWGYY77 pKa = 10.94VPATEE82 pKa = 4.26EE83 pKa = 4.31SRR85 pKa = 11.84FEE87 pKa = 4.17NGWWEE92 pKa = 4.13LQWASYY98 pKa = 10.59EE99 pKa = 4.07DD100 pKa = 5.2AKK102 pKa = 11.14AAWDD106 pKa = 3.41EE107 pKa = 4.09WTQDD111 pKa = 3.0EE112 pKa = 4.38EE113 pKa = 5.86AIAFLADD120 pKa = 3.52HH121 pKa = 6.85EE122 pKa = 4.98SVMVCDD128 pKa = 4.22GDD130 pKa = 5.47ARR132 pKa = 11.84GSWDD136 pKa = 3.32FTFHH140 pKa = 7.5RR141 pKa = 11.84DD142 pKa = 3.38VEE144 pKa = 4.93SFGPMPEE151 pKa = 4.67DD152 pKa = 3.27GSFFTEE158 pKa = 5.4FFPCKK163 pKa = 10.39YY164 pKa = 10.28NDD166 pKa = 3.7GKK168 pKa = 11.23SSEE171 pKa = 4.67DD172 pKa = 3.45LLGSIALYY180 pKa = 10.69NEE182 pKa = 3.94WLDD185 pKa = 3.53GLEE188 pKa = 3.8ITGAYY193 pKa = 10.08AYY195 pKa = 10.12GIYY198 pKa = 10.52AGDD201 pKa = 4.01GTDD204 pKa = 3.51EE205 pKa = 5.54LADD208 pKa = 3.99FWWGNFHH215 pKa = 7.3EE216 pKa = 4.82SAEE219 pKa = 4.26SAAVGNEE226 pKa = 3.04NWMEE230 pKa = 4.17SGGKK234 pKa = 9.58ARR236 pKa = 11.84ASLEE240 pKa = 3.73ATAACDD246 pKa = 3.89VPEE249 pKa = 5.03LYY251 pKa = 10.68NSGVIYY257 pKa = 10.62DD258 pKa = 3.98PARR261 pKa = 11.84PAFAKK266 pKa = 10.52SS267 pKa = 3.03

Molecular weight: 29.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6GIN0|K6GIN0_9GAMM RNA polymerase sigma factor RpoH OS=SAR86 cluster bacterium SAR86E OX=1208365 GN=rpoH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 7.79RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19AGRR28 pKa = 11.84AVIANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 10.05KK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.97VLTAA44 pKa = 4.27

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1403

0

1403

442143

37

1507

315.1

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.038 ± 0.052

0.951 ± 0.02

5.785 ± 0.053

6.523 ± 0.058

4.918 ± 0.05

6.846 ± 0.062

1.917 ± 0.034

8.244 ± 0.068

6.812 ± 0.073

9.795 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.03

5.451 ± 0.047

3.784 ± 0.034

3.418 ± 0.035

3.595 ± 0.047

7.784 ± 0.057

4.786 ± 0.043

5.763 ± 0.052

1.073 ± 0.024

3.068 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski