Gokushovirinae Bog8989_22

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UEC9|A0A0G2UEC9_9VIRU DNA binding protein VP5 OS=Gokushovirinae Bog8989_22 OX=1655650 PE=4 SV=1
MM1 pKa = 7.6SSASLISQHH10 pKa = 5.87SFAIAPHH17 pKa = 6.4PKK19 pKa = 9.96LPRR22 pKa = 11.84AIFNRR27 pKa = 11.84TFTHH31 pKa = 6.23KK32 pKa = 6.69TTCNAGMLIPFLIDD46 pKa = 3.77EE47 pKa = 5.35IYY49 pKa = 10.75PGDD52 pKa = 3.85TFKK55 pKa = 11.18LRR57 pKa = 11.84STFLARR63 pKa = 11.84MSTPIYY69 pKa = 10.26PVMDD73 pKa = 3.77NLFIDD78 pKa = 3.79TQFFFIPNRR87 pKa = 11.84LLWTHH92 pKa = 6.54WEE94 pKa = 3.86QFMGYY99 pKa = 8.36QEE101 pKa = 4.63NPGDD105 pKa = 4.4SIDD108 pKa = 3.83YY109 pKa = 7.34TLPTITSPTGGYY121 pKa = 10.02LEE123 pKa = 4.06QTIYY127 pKa = 11.02DD128 pKa = 4.09YY129 pKa = 11.63LGLPTKK135 pKa = 10.33IAGVTHH141 pKa = 5.8QALPLRR147 pKa = 11.84AYY149 pKa = 10.8NKK151 pKa = 9.96VYY153 pKa = 10.69NDD155 pKa = 3.32WYY157 pKa = 10.79RR158 pKa = 11.84DD159 pKa = 3.48QNLQDD164 pKa = 4.32SVVLPNGDD172 pKa = 3.82GPDD175 pKa = 3.47APTDD179 pKa = 3.73YY180 pKa = 11.46VLLPRR185 pKa = 11.84GKK187 pKa = 9.2RR188 pKa = 11.84HH189 pKa = 7.56DD190 pKa = 4.31YY191 pKa = 8.14FTSALPTPQKK201 pKa = 10.58GAAVQLPLGTTAPILSNNTQVNFSDD226 pKa = 3.97EE227 pKa = 4.72AMTSPDD233 pKa = 3.1HH234 pKa = 6.68GLYY237 pKa = 10.15GQEE240 pKa = 3.99GTNLLVLGGSAFGATEE256 pKa = 3.64PLIFGNNTGLYY267 pKa = 9.98ADD269 pKa = 5.02LSSATSATINDD280 pKa = 3.91LRR282 pKa = 11.84LSIATQQYY290 pKa = 9.68LEE292 pKa = 4.12RR293 pKa = 11.84DD294 pKa = 3.49MRR296 pKa = 11.84SGTRR300 pKa = 11.84YY301 pKa = 8.29TEE303 pKa = 3.79VLLGHH308 pKa = 6.99WGIAAQDD315 pKa = 3.37ARR317 pKa = 11.84LQRR320 pKa = 11.84SEE322 pKa = 4.0YY323 pKa = 10.51LGGGSTQINFHH334 pKa = 6.69PVAQTAPSDD343 pKa = 3.48ASGSGEE349 pKa = 3.82EE350 pKa = 4.63SSPQGTLSAFATAVGYY366 pKa = 10.1DD367 pKa = 3.15HH368 pKa = 7.31GFTKK372 pKa = 10.58SFPEE376 pKa = 3.8HH377 pKa = 6.51GFILGLMSIRR387 pKa = 11.84ADD389 pKa = 3.31YY390 pKa = 9.34TYY392 pKa = 10.8QQGAEE397 pKa = 3.91RR398 pKa = 11.84FWFRR402 pKa = 11.84KK403 pKa = 9.7ARR405 pKa = 11.84FDD407 pKa = 4.17LYY409 pKa = 10.78TPEE412 pKa = 3.99FANLGEE418 pKa = 4.11QAVLNQEE425 pKa = 4.66IYY427 pKa = 10.92AQGTSADD434 pKa = 3.91QEE436 pKa = 4.14PFGYY440 pKa = 9.9QEE442 pKa = 3.42AWAEE446 pKa = 3.95LRR448 pKa = 11.84YY449 pKa = 9.99KK450 pKa = 10.38PSRR453 pKa = 11.84ISGYY457 pKa = 9.7FRR459 pKa = 11.84SNATGTLDD467 pKa = 3.09AWHH470 pKa = 7.33LSQEE474 pKa = 4.46FTSPPTLSPAFIIEE488 pKa = 4.47NPPMARR494 pKa = 11.84VEE496 pKa = 4.17AVSDD500 pKa = 3.69VCDD503 pKa = 5.75FIVDD507 pKa = 4.06SNNKK511 pKa = 7.46FHH513 pKa = 7.85CIRR516 pKa = 11.84QIGMYY521 pKa = 9.53SVPGLYY527 pKa = 10.22RR528 pKa = 11.84LL529 pKa = 3.85

Molecular weight: 58.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UK17|A0A0G2UK17_9VIRU Replication protein VP4 OS=Gokushovirinae Bog8989_22 OX=1655650 PE=4 SV=1
MM1 pKa = 7.53GLFDD5 pKa = 6.2AIGSAVGAGLAYY17 pKa = 10.17EE18 pKa = 4.67GVKK21 pKa = 8.83EE22 pKa = 4.1TNQANKK28 pKa = 10.44DD29 pKa = 3.32INNANNTFSAQQADD43 pKa = 3.43INRR46 pKa = 11.84NFQEE50 pKa = 4.16RR51 pKa = 11.84MSDD54 pKa = 3.11SQYY57 pKa = 10.09QRR59 pKa = 11.84QMADD63 pKa = 2.74MRR65 pKa = 11.84LAGLNPILAASNGGGAGTPSGSAASAGPSPTMQNPMASAVSSAQSVAQTANSIASADD122 pKa = 3.88LTRR125 pKa = 11.84QLALKK130 pKa = 10.45ASAEE134 pKa = 4.27TKK136 pKa = 10.76SIDD139 pKa = 3.55VNSAKK144 pKa = 10.41NAATAPIYY152 pKa = 10.23RR153 pKa = 11.84AAGEE157 pKa = 4.3VTTKK161 pKa = 10.83GLSLWDD167 pKa = 3.62RR168 pKa = 11.84FVPLAWSALSAVPKK182 pKa = 10.43KK183 pKa = 10.9LGGALYY189 pKa = 10.43NGRR192 pKa = 11.84EE193 pKa = 4.5YY194 pKa = 11.27YY195 pKa = 10.16RR196 pKa = 11.84AAHH199 pKa = 5.89NRR201 pKa = 11.84PSAKK205 pKa = 9.66TSWGASGAPSDD216 pKa = 3.87WQPP219 pKa = 2.91

Molecular weight: 22.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1292

73

529

215.3

23.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.669 ± 2.15

1.316 ± 0.739

5.805 ± 0.573

3.87 ± 0.268

5.108 ± 0.825

6.811 ± 0.832

2.012 ± 0.446

4.954 ± 0.476

4.025 ± 0.964

9.52 ± 0.844

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.935 ± 0.214

4.567 ± 0.675

6.347 ± 0.378

4.334 ± 0.766

5.573 ± 0.939

8.359 ± 0.664

6.115 ± 0.978

4.489 ± 1.007

1.006 ± 0.303

5.186 ± 0.917
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski