Amino acid dipepetide frequency for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker s yeast)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.973AlaAla: 3.973 ± 0.055
0.733AlaCys: 0.733 ± 0.018
2.644AlaAsp: 2.644 ± 0.033
3.055AlaGlu: 3.055 ± 0.038
2.276AlaPhe: 2.276 ± 0.031
2.812AlaGly: 2.812 ± 0.039
1.151AlaHis: 1.151 ± 0.022
3.676AlaIle: 3.676 ± 0.042
3.878AlaLys: 3.878 ± 0.043
5.252AlaLeu: 5.252 ± 0.053
1.264AlaMet: 1.264 ± 0.023
3.157AlaAsn: 3.157 ± 0.036
2.365AlaPro: 2.365 ± 0.037
2.115AlaGln: 2.115 ± 0.037
2.425AlaArg: 2.425 ± 0.034
5.143AlaSer: 5.143 ± 0.054
3.598AlaThr: 3.598 ± 0.052
3.186AlaVal: 3.186 ± 0.041
0.505AlaTrp: 0.505 ± 0.015
1.722AlaTyr: 1.722 ± 0.028
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.018
0.296CysCys: 0.296 ± 0.011
0.657CysAsp: 0.657 ± 0.018
0.614CysGlu: 0.614 ± 0.015
0.682CysPhe: 0.682 ± 0.017
0.824CysGly: 0.824 ± 0.019
0.333CysHis: 0.333 ± 0.012
0.936CysIle: 0.936 ± 0.019
0.755CysLys: 0.755 ± 0.016
1.327CysLeu: 1.327 ± 0.025
0.279CysMet: 0.279 ± 0.009
0.598CysAsn: 0.598 ± 0.016
0.562CysPro: 0.562 ± 0.016
0.499CysGln: 0.499 ± 0.014
0.523CysArg: 0.523 ± 0.013
1.105CysSer: 1.105 ± 0.02
0.66CysThr: 0.66 ± 0.017
0.751CysVal: 0.751 ± 0.016
0.152CysTrp: 0.152 ± 0.007
0.476CysTyr: 0.476 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.118AspAla: 3.118 ± 0.032
0.668AspCys: 0.668 ± 0.016
4.64AspAsp: 4.64 ± 0.065
5.012AspGlu: 5.012 ± 0.057
2.704AspPhe: 2.704 ± 0.032
2.85AspGly: 2.85 ± 0.037
1.105AspHis: 1.105 ± 0.019
4.366AspIle: 4.366 ± 0.039
3.52AspLys: 3.52 ± 0.041
5.581AspLeu: 5.581 ± 0.047
1.228AspMet: 1.228 ± 0.019
3.23AspAsn: 3.23 ± 0.04
2.451AspPro: 2.451 ± 0.028
1.722AspGln: 1.722 ± 0.027
2.107AspArg: 2.107 ± 0.029
4.912AspSer: 4.912 ± 0.052
3.022AspThr: 3.022 ± 0.039
3.418AspVal: 3.418 ± 0.036
0.632AspTrp: 0.632 ± 0.018
2.088AspTyr: 2.088 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
3.426GluAla: 3.426 ± 0.042
0.659GluCys: 0.659 ± 0.016
4.324GluAsp: 4.324 ± 0.052
5.928GluGlu: 5.928 ± 0.082
2.684GluPhe: 2.684 ± 0.035
2.704GluGly: 2.704 ± 0.032
1.219GluHis: 1.219 ± 0.021
4.317GluIle: 4.317 ± 0.04
5.413GluLys: 5.413 ± 0.058
6.294GluLeu: 6.294 ± 0.055
1.379GluMet: 1.379 ± 0.023
4.454GluAsn: 4.454 ± 0.046
2.078GluPro: 2.078 ± 0.026
2.564GluGln: 2.564 ± 0.034
2.97GluArg: 2.97 ± 0.037
4.911GluSer: 4.911 ± 0.053
3.655GluThr: 3.655 ± 0.036
3.4GluVal: 3.4 ± 0.035
0.628GluTrp: 0.628 ± 0.016
2.228GluTyr: 2.228 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
2.367PheAla: 2.367 ± 0.036
0.576PheCys: 0.576 ± 0.015
2.672PheAsp: 2.672 ± 0.036
2.766PheGlu: 2.766 ± 0.034
2.173PhePhe: 2.173 ± 0.039
2.496PheGly: 2.496 ± 0.047
1.056PheHis: 1.056 ± 0.022
3.035PheIle: 3.035 ± 0.038
3.01PheLys: 3.01 ± 0.036
4.288PheLeu: 4.288 ± 0.044
0.906PheMet: 0.906 ± 0.018
2.61PheAsn: 2.61 ± 0.033
1.903PhePro: 1.903 ± 0.026
1.992PheGln: 1.992 ± 0.026
1.697PheArg: 1.697 ± 0.028
3.606PheSer: 3.606 ± 0.039
2.543PheThr: 2.543 ± 0.036
2.489PheVal: 2.489 ± 0.038
0.516PheTrp: 0.516 ± 0.015
1.641PheTyr: 1.641 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
2.76GlyAla: 2.76 ± 0.041
0.741GlyCys: 0.741 ± 0.018
2.696GlyAsp: 2.696 ± 0.032
2.853GlyGlu: 2.853 ± 0.033
2.273GlyPhe: 2.273 ± 0.032
3.115GlyGly: 3.115 ± 0.046
1.115GlyHis: 1.115 ± 0.021
3.439GlyIle: 3.439 ± 0.041
3.713GlyLys: 3.713 ± 0.036
4.552GlyLeu: 4.552 ± 0.044
1.054GlyMet: 1.054 ± 0.019
2.927GlyAsn: 2.927 ± 0.038
1.683GlyPro: 1.683 ± 0.026
1.663GlyGln: 1.663 ± 0.03
2.084GlyArg: 2.084 ± 0.033
4.628GlySer: 4.628 ± 0.048
3.012GlyThr: 3.012 ± 0.036
2.977GlyVal: 2.977 ± 0.036
0.601GlyTrp: 0.601 ± 0.015
1.821GlyTyr: 1.821 ± 0.028
0.0GlyXaa: 0.0 ± 0.0
His
1.072HisAla: 1.072 ± 0.02
0.339HisCys: 0.339 ± 0.012
1.253HisAsp: 1.253 ± 0.021
1.274HisGlu: 1.274 ± 0.021
0.973HisPhe: 0.973 ± 0.018
1.162HisGly: 1.162 ± 0.019
0.74HisHis: 0.74 ± 0.022
1.43HisIle: 1.43 ± 0.024
1.319HisLys: 1.319 ± 0.023
2.19HisLeu: 2.19 ± 0.029
0.43HisMet: 0.43 ± 0.012
1.205HisAsn: 1.205 ± 0.023
1.138HisPro: 1.138 ± 0.022
0.824HisGln: 0.824 ± 0.017
1.024HisArg: 1.024 ± 0.022
1.964HisSer: 1.964 ± 0.032
1.152HisThr: 1.152 ± 0.024
1.159HisVal: 1.159 ± 0.021
0.256HisTrp: 0.256 ± 0.01
0.813HisTyr: 0.813 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
3.624IleAla: 3.624 ± 0.035
0.935IleCys: 0.935 ± 0.018
3.987IleAsp: 3.987 ± 0.039
4.079IleGlu: 4.079 ± 0.035
3.006IlePhe: 3.006 ± 0.037
3.129IleGly: 3.129 ± 0.041
1.455IleHis: 1.455 ± 0.026
4.345IleIle: 4.345 ± 0.051
4.627IleLys: 4.627 ± 0.041
6.321IleLeu: 6.321 ± 0.059
1.386IleMet: 1.386 ± 0.022
3.945IleAsn: 3.945 ± 0.041
3.294IlePro: 3.294 ± 0.039
2.409IleGln: 2.409 ± 0.032
2.804IleArg: 2.804 ± 0.031
5.914IleSer: 5.914 ± 0.046
3.775IleThr: 3.775 ± 0.04
3.679IleVal: 3.679 ± 0.036
0.747IleTrp: 0.747 ± 0.018
2.11IleTyr: 2.11 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
3.67LysAla: 3.67 ± 0.038
0.825LysCys: 0.825 ± 0.02
4.167LysAsp: 4.167 ± 0.043
5.37LysGlu: 5.37 ± 0.062
3.291LysPhe: 3.291 ± 0.039
2.986LysGly: 2.986 ± 0.034
1.467LysHis: 1.467 ± 0.021
4.85LysIle: 4.85 ± 0.047
6.781LysLys: 6.781 ± 0.07
7.051LysLeu: 7.051 ± 0.051
1.53LysMet: 1.53 ± 0.024
4.551LysAsn: 4.551 ± 0.04
2.987LysPro: 2.987 ± 0.037
2.76LysGln: 2.76 ± 0.035
3.997LysArg: 3.997 ± 0.039
5.647LysSer: 5.647 ± 0.055
4.016LysThr: 4.016 ± 0.039
3.965LysVal: 3.965 ± 0.041
0.77LysTrp: 0.77 ± 0.016
2.744LysTyr: 2.744 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
5.276LeuAla: 5.276 ± 0.053
1.317LeuCys: 1.317 ± 0.023
5.324LeuAsp: 5.324 ± 0.05
5.997LeuGlu: 5.997 ± 0.062
4.17LeuPhe: 4.17 ± 0.047
4.472LeuGly: 4.472 ± 0.04
2.151LeuHis: 2.151 ± 0.03
5.785LeuIle: 5.785 ± 0.051
7.442LeuLys: 7.442 ± 0.069
9.386LeuLeu: 9.386 ± 0.089
1.927LeuMet: 1.927 ± 0.029
5.782LeuAsn: 5.782 ± 0.055
4.712LeuPro: 4.712 ± 0.043
4.361LeuGln: 4.361 ± 0.047
4.448LeuArg: 4.448 ± 0.045
8.32LeuSer: 8.32 ± 0.053
5.223LeuThr: 5.223 ± 0.046
4.991LeuVal: 4.991 ± 0.05
0.896LeuTrp: 0.896 ± 0.018
3.089LeuTyr: 3.089 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
1.352MetAla: 1.352 ± 0.022
0.244MetCys: 0.244 ± 0.009
1.308MetAsp: 1.308 ± 0.024
1.351MetGlu: 1.351 ± 0.023
0.866MetPhe: 0.866 ± 0.02
1.155MetGly: 1.155 ± 0.023
0.381MetHis: 0.381 ± 0.012
1.312MetIle: 1.312 ± 0.019
1.587MetLys: 1.587 ± 0.026
1.798MetLeu: 1.798 ± 0.028
0.523MetMet: 0.523 ± 0.015
1.344MetAsn: 1.344 ± 0.025
0.789MetPro: 0.789 ± 0.019
0.755MetGln: 0.755 ± 0.017
0.877MetArg: 0.877 ± 0.017
1.9MetSer: 1.9 ± 0.026
1.328MetThr: 1.328 ± 0.021
1.215MetVal: 1.215 ± 0.023
0.187MetTrp: 0.187 ± 0.009
0.588MetTyr: 0.588 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.216AsnAla: 3.216 ± 0.039
0.715AsnCys: 0.715 ± 0.016
4.08AsnAsp: 4.08 ± 0.047
4.28AsnGlu: 4.28 ± 0.046
2.583AsnPhe: 2.583 ± 0.037
3.489AsnGly: 3.489 ± 0.04
1.235AsnHis: 1.235 ± 0.021
4.082AsnIle: 4.082 ± 0.042
4.322AsnLys: 4.322 ± 0.045
5.149AsnLeu: 5.149 ± 0.047
1.258AsnMet: 1.258 ± 0.02
4.878AsnAsn: 4.878 ± 0.091
2.443AsnPro: 2.443 ± 0.033
1.969AsnGln: 1.969 ± 0.027
2.264AsnArg: 2.264 ± 0.028
5.898AsnSer: 5.898 ± 0.073
3.534AsnThr: 3.534 ± 0.042
3.457AsnVal: 3.457 ± 0.039
0.667AsnTrp: 0.667 ± 0.016
2.043AsnTyr: 2.043 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
2.243ProAla: 2.243 ± 0.035
0.386ProCys: 0.386 ± 0.013
2.181ProAsp: 2.181 ± 0.025
2.901ProGlu: 2.901 ± 0.032
1.92ProPhe: 1.92 ± 0.03
1.923ProGly: 1.923 ± 0.028
1.021ProHis: 1.021 ± 0.02
2.897ProIle: 2.897 ± 0.032
2.889ProLys: 2.889 ± 0.032
4.198ProLeu: 4.198 ± 0.037
0.861ProMet: 0.861 ± 0.015
2.514ProAsn: 2.514 ± 0.032
2.507ProPro: 2.507 ± 0.061
1.994ProGln: 1.994 ± 0.038
1.793ProArg: 1.793 ± 0.025
4.321ProSer: 4.321 ± 0.053
2.777ProThr: 2.777 ± 0.04
2.595ProVal: 2.595 ± 0.035
0.444ProTrp: 0.444 ± 0.014
1.441ProTyr: 1.441 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
1.952GlnAla: 1.952 ± 0.036
0.463GlnCys: 0.463 ± 0.013
2.103GlnAsp: 2.103 ± 0.031
2.778GlnGlu: 2.778 ± 0.033
1.764GlnPhe: 1.764 ± 0.027
1.73GlnGly: 1.73 ± 0.027
0.9GlnHis: 0.9 ± 0.021
2.459GlnIle: 2.459 ± 0.026
2.753GlnLys: 2.753 ± 0.03
4.073GlnLeu: 4.073 ± 0.041
0.866GlnMet: 0.866 ± 0.018
2.385GlnAsn: 2.385 ± 0.034
1.617GlnPro: 1.617 ± 0.031
2.876GlnGln: 2.876 ± 0.093
1.999GlnArg: 1.999 ± 0.026
2.911GlnSer: 2.911 ± 0.035
1.99GlnThr: 1.99 ± 0.027
1.993GlnVal: 1.993 ± 0.027
0.417GlnTrp: 0.417 ± 0.012
1.473GlnTyr: 1.473 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
2.282ArgAla: 2.282 ± 0.031
0.588ArgCys: 0.588 ± 0.014
2.443ArgAsp: 2.443 ± 0.033
2.756ArgGlu: 2.756 ± 0.036
1.935ArgPhe: 1.935 ± 0.026
2.161ArgGly: 2.161 ± 0.043
1.045ArgHis: 1.045 ± 0.02
2.889ArgIle: 2.889 ± 0.036
3.698ArgLys: 3.698 ± 0.045
4.239ArgLeu: 4.239 ± 0.044
0.94ArgMet: 0.94 ± 0.02
2.768ArgAsn: 2.768 ± 0.033
1.687ArgPro: 1.687 ± 0.025
1.771ArgGln: 1.771 ± 0.026
2.8ArgArg: 2.8 ± 0.04
3.695ArgSer: 3.695 ± 0.041
2.466ArgThr: 2.466 ± 0.031
2.227ArgVal: 2.227 ± 0.03
0.473ArgTrp: 0.473 ± 0.013
1.576ArgTyr: 1.576 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
4.849SerAla: 4.849 ± 0.053
0.99SerCys: 0.99 ± 0.021
4.666SerAsp: 4.666 ± 0.048
4.682SerGlu: 4.682 ± 0.054
3.778SerPhe: 3.778 ± 0.043
4.351SerGly: 4.351 ± 0.046
1.915SerHis: 1.915 ± 0.027
5.613SerIle: 5.613 ± 0.048
6.559SerLys: 6.559 ± 0.055
8.339SerLeu: 8.339 ± 0.061
1.857SerMet: 1.857 ± 0.026
5.814SerAsn: 5.814 ± 0.071
3.925SerPro: 3.925 ± 0.059
3.548SerGln: 3.548 ± 0.037
3.945SerArg: 3.945 ± 0.041
10.719SerSer: 10.719 ± 0.196
6.26SerThr: 6.26 ± 0.092
4.62SerVal: 4.62 ± 0.039
0.795SerTrp: 0.795 ± 0.017
2.582SerTyr: 2.582 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
3.507ThrAla: 3.507 ± 0.037
0.67ThrCys: 0.67 ± 0.018
3.075ThrAsp: 3.075 ± 0.036
3.341ThrGlu: 3.341 ± 0.042
2.604ThrPhe: 2.604 ± 0.032
3.054ThrGly: 3.054 ± 0.04
1.175ThrHis: 1.175 ± 0.021
3.784ThrIle: 3.784 ± 0.038
4.164ThrLys: 4.164 ± 0.039
5.44ThrLeu: 5.44 ± 0.044
1.075ThrMet: 1.075 ± 0.02
3.708ThrAsn: 3.708 ± 0.054
3.052ThrPro: 3.052 ± 0.04
1.951ThrGln: 1.951 ± 0.025
2.385ThrArg: 2.385 ± 0.027
5.75ThrSer: 5.75 ± 0.087
4.475ThrThr: 4.475 ± 0.091
3.556ThrVal: 3.556 ± 0.039
0.626ThrTrp: 0.626 ± 0.017
1.74ThrTyr: 1.74 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
3.364ValAla: 3.364 ± 0.044
0.77ValCys: 0.77 ± 0.018
3.348ValAsp: 3.348 ± 0.037
3.516ValGlu: 3.516 ± 0.043
2.463ValPhe: 2.463 ± 0.035
2.879ValGly: 2.879 ± 0.042
1.193ValHis: 1.193 ± 0.021
3.547ValIle: 3.547 ± 0.042
3.865ValLys: 3.865 ± 0.033
5.264ValLeu: 5.264 ± 0.05
1.119ValMet: 1.119 ± 0.021
3.059ValAsn: 3.059 ± 0.034
2.882ValPro: 2.882 ± 0.04
1.963ValGln: 1.963 ± 0.027
2.359ValArg: 2.359 ± 0.033
4.889ValSer: 4.889 ± 0.047
3.294ValThr: 3.294 ± 0.039
3.411ValVal: 3.411 ± 0.043
0.552ValTrp: 0.552 ± 0.015
1.844ValTyr: 1.844 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.518TrpAla: 0.518 ± 0.014
0.23TrpCys: 0.23 ± 0.009
0.658TrpAsp: 0.658 ± 0.016
0.638TrpGlu: 0.638 ± 0.016
0.519TrpPhe: 0.519 ± 0.015
0.507TrpGly: 0.507 ± 0.015
0.22TrpHis: 0.22 ± 0.009
0.639TrpIle: 0.639 ± 0.015
0.875TrpLys: 0.875 ± 0.022
0.985TrpLeu: 0.985 ± 0.018
0.226TrpMet: 0.226 ± 0.008
0.666TrpAsn: 0.666 ± 0.014
0.301TrpPro: 0.301 ± 0.01
0.38TrpGln: 0.38 ± 0.01
0.503TrpArg: 0.503 ± 0.014
0.886TrpSer: 0.886 ± 0.019
0.512TrpThr: 0.512 ± 0.013
0.615TrpVal: 0.615 ± 0.016
0.147TrpTrp: 0.147 ± 0.008
0.382TrpTyr: 0.382 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.704TyrAla: 1.704 ± 0.025
0.543TyrCys: 0.543 ± 0.015
2.147TyrAsp: 2.147 ± 0.029
2.042TyrGlu: 2.042 ± 0.029
1.68TyrPhe: 1.68 ± 0.032
1.909TyrGly: 1.909 ± 0.029
0.838TyrHis: 0.838 ± 0.016
2.168TyrIle: 2.168 ± 0.028
2.253TyrLys: 2.253 ± 0.029
3.342TyrLeu: 3.342 ± 0.038
0.753TyrMet: 0.753 ± 0.015
1.966TyrAsn: 1.966 ± 0.028
1.43TyrPro: 1.43 ± 0.025
1.384TyrGln: 1.384 ± 0.024
1.437TyrArg: 1.437 ± 0.02
2.689TyrSer: 2.689 ± 0.036
1.841TyrThr: 1.841 ± 0.025
1.877TyrVal: 1.877 ± 0.026
0.398TyrTrp: 0.398 ± 0.013
1.452TyrTyr: 1.452 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6079 proteins (2949596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski