Lactobacillus pasteurii DSM 23907 = CRBIP 24.76

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus pasteurii

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7KLE6|I7KLE6_9LACO Uncharacterized protein OS=Lactobacillus pasteurii DSM 23907 = CRBIP 24.76 OX=1423790 GN=BN53_04425 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 6.75AIQFLYY8 pKa = 10.49QSSGWSGPSSYY19 pKa = 10.47TIYY22 pKa = 11.13GLYY25 pKa = 10.7NSDD28 pKa = 3.96PNGGHH33 pKa = 5.76QRR35 pKa = 11.84LTAADD40 pKa = 4.37TIYY43 pKa = 10.79QSNDD47 pKa = 2.56YY48 pKa = 9.63DD49 pKa = 4.08VPVAIIDD56 pKa = 3.62VDD58 pKa = 3.8TKK60 pKa = 9.7TEE62 pKa = 3.83KK63 pKa = 10.79KK64 pKa = 10.98LNVQLNNPSLQGSWNLDD81 pKa = 3.46EE82 pKa = 5.4LLKK85 pKa = 11.27LSDD88 pKa = 3.71DD89 pKa = 3.88VSFEE93 pKa = 4.14EE94 pKa = 4.65MGFDD98 pKa = 5.04QSDD101 pKa = 3.52IDD103 pKa = 4.53NMFDD107 pKa = 3.36GQVAFSSDD115 pKa = 4.25DD116 pKa = 4.0DD117 pKa = 5.02PDD119 pKa = 4.58DD120 pKa = 5.69LSDD123 pKa = 4.51PLTGNADD130 pKa = 3.67DD131 pKa = 4.8SDD133 pKa = 4.57SSFEE137 pKa = 4.12PSFQNAQKK145 pKa = 11.26ALFEE149 pKa = 4.22FADD152 pKa = 3.57NFTYY156 pKa = 10.56QQVKK160 pKa = 6.87FHH162 pKa = 6.95CNPVVDD168 pKa = 3.83LHH170 pKa = 7.65VYY172 pKa = 9.79AA173 pKa = 5.85

Molecular weight: 19.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7LBV7|I7LBV7_9LACO Membrane protein OS=Lactobacillus pasteurii DSM 23907 = CRBIP 24.76 OX=1423790 GN=BN53_07570 PE=3 SV=1
MM1 pKa = 7.87PKK3 pKa = 9.59MKK5 pKa = 8.8THH7 pKa = 6.78RR8 pKa = 11.84ASAKK12 pKa = 9.47RR13 pKa = 11.84FKK15 pKa = 10.03RR16 pKa = 11.84TANGGLKK23 pKa = 9.5RR24 pKa = 11.84HH25 pKa = 6.15HH26 pKa = 7.23AFTGHH31 pKa = 6.77RR32 pKa = 11.84FHH34 pKa = 7.54GKK36 pKa = 6.24TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.45LRR45 pKa = 11.84KK46 pKa = 8.75AAMVSRR52 pKa = 11.84SDD54 pKa = 3.16IKK56 pKa = 10.64RR57 pKa = 11.84IKK59 pKa = 10.62QMLSQMRR66 pKa = 3.59

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1825

0

1825

549035

30

2261

300.8

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.559 ± 0.071

0.52 ± 0.014

6.107 ± 0.06

5.961 ± 0.06

4.297 ± 0.046

6.447 ± 0.053

1.908 ± 0.027

7.495 ± 0.064

7.643 ± 0.072

9.679 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.033

5.101 ± 0.05

3.418 ± 0.036

4.139 ± 0.048

3.779 ± 0.045

6.229 ± 0.055

5.504 ± 0.05

6.711 ± 0.043

1.007 ± 0.022

3.896 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski