Simian hemorrhagic fever virus (SHFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Simarterivirinae; Deltaarterivirus; Hedartevirus; Deltaarterivirus hemfev

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|P89136|P89136_SHFV E protein OS=Simian hemorrhagic fever virus OX=38143 GN=ORF2a PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 6.31RR3 pKa = 11.84DD4 pKa = 3.03NDD6 pKa = 3.56VCKK9 pKa = 10.33RR10 pKa = 11.84HH11 pKa = 6.06CSVDD15 pKa = 2.73KK16 pKa = 10.49RR17 pKa = 11.84YY18 pKa = 10.12CGRR21 pKa = 11.84VLHH24 pKa = 5.69PHH26 pKa = 6.98GGRR29 pKa = 11.84FAYY32 pKa = 9.09VLCGIVAALSSVPANSNAEE51 pKa = 3.74RR52 pKa = 11.84CMICTTNNFSTLFTEE67 pKa = 5.13SSYY70 pKa = 9.38QHH72 pKa = 5.68YY73 pKa = 9.41PRR75 pKa = 11.84WPVFQDD81 pKa = 3.04GLDD84 pKa = 3.58YY85 pKa = 10.94VYY87 pKa = 10.6RR88 pKa = 11.84PPLVAQVLGYY98 pKa = 11.23GEE100 pKa = 4.63TCDD103 pKa = 3.5DD104 pKa = 3.67TQIIGTIIEE113 pKa = 4.43TYY115 pKa = 8.51EE116 pKa = 4.25TITGNMTGIKK126 pKa = 9.44EE127 pKa = 4.0AFIALDD133 pKa = 3.69FADD136 pKa = 4.91CLLTGILYY144 pKa = 10.22RR145 pKa = 11.84EE146 pKa = 4.42HH147 pKa = 6.72NVTAVFQQQDD157 pKa = 3.39GHH159 pKa = 7.68IVLCWNGTDD168 pKa = 3.62PRR170 pKa = 11.84ISINQIPTPWFISPGALRR188 pKa = 11.84WATIICACLAIFRR201 pKa = 11.84AFYY204 pKa = 10.88SS205 pKa = 3.52

Molecular weight: 23.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q87063|Q87063_SHFV Major structural protein OS=Simian hemorrhagic fever virus OX=38143 GN=ORF7 PE=4 SV=1
MM1 pKa = 7.08VVSLCSDD8 pKa = 3.88PGYY11 pKa = 6.96TTLAFTIAPALIAFLRR27 pKa = 11.84YY28 pKa = 8.76FRR30 pKa = 11.84PSVRR34 pKa = 11.84GFICLVCIATLAYY47 pKa = 10.16AATAFNEE54 pKa = 4.16HH55 pKa = 6.45SLATLLTIGFSLVYY69 pKa = 9.13LTYY72 pKa = 11.07KK73 pKa = 10.68FITWTILRR81 pKa = 11.84VRR83 pKa = 11.84MCWLGRR89 pKa = 11.84QYY91 pKa = 9.2ITAPSSMVEE100 pKa = 3.92SSLGRR105 pKa = 11.84LAINATGSTAVVTRR119 pKa = 11.84RR120 pKa = 11.84SGMTAVNGSLMPDD133 pKa = 3.52VKK135 pKa = 10.65RR136 pKa = 11.84IILNGRR142 pKa = 11.84VAAKK146 pKa = 10.13RR147 pKa = 11.84GLVNLRR153 pKa = 11.84KK154 pKa = 10.32YY155 pKa = 10.37GWQTKK160 pKa = 9.44NKK162 pKa = 9.82

Molecular weight: 17.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

1

14

7753

64

3595

553.8

60.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.603 ± 0.633

3.599 ± 0.253

4.514 ± 0.595

3.341 ± 0.223

4.618 ± 0.349

6.784 ± 0.701

2.954 ± 0.305

5.275 ± 0.608

4.166 ± 0.493

10.344 ± 0.782

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.316 ± 0.124

3.263 ± 0.358

6.475 ± 0.517

2.76 ± 0.338

4.876 ± 0.308

7.674 ± 0.476

6.552 ± 0.569

8.023 ± 0.638

1.135 ± 0.138

3.676 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski