Propionibacteriaceae bacterium NML 130396

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; unclassified Propionibacteriaceae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255H350|A0A255H350_9ACTN Uncharacterized protein OS=Propionibacteriaceae bacterium NML 130396 OX=2016507 GN=CGZ93_09570 PE=4 SV=1
MM1 pKa = 7.71AEE3 pKa = 3.96YY4 pKa = 10.28PEE6 pKa = 4.24DD7 pKa = 3.22LKK9 pKa = 11.48YY10 pKa = 10.89SSEE13 pKa = 4.39HH14 pKa = 4.68EE15 pKa = 4.03WVRR18 pKa = 11.84PGNGLTLRR26 pKa = 11.84VGISEE31 pKa = 4.53FAAEE35 pKa = 3.92QLGDD39 pKa = 3.42IVYY42 pKa = 10.14VQLPDD47 pKa = 3.35VGQEE51 pKa = 3.77MAAGDD56 pKa = 3.98SVGEE60 pKa = 4.1LEE62 pKa = 4.45STKK65 pKa = 10.73SVSDD69 pKa = 3.23LMAPVAGVVTGINEE83 pKa = 4.29AVVDD87 pKa = 4.26APEE90 pKa = 4.66TINADD95 pKa = 3.54PYY97 pKa = 9.82GDD99 pKa = 3.08GWLFEE104 pKa = 4.29VEE106 pKa = 4.51VAGEE110 pKa = 3.73EE111 pKa = 4.39AYY113 pKa = 10.73ADD115 pKa = 4.57LMDD118 pKa = 4.87ADD120 pKa = 5.19AYY122 pKa = 10.7AEE124 pKa = 4.33HH125 pKa = 7.56VGEE128 pKa = 4.27

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255HBH5|A0A255HBH5_9ACTN Polyamine aminopropyltransferase OS=Propionibacteriaceae bacterium NML 130396 OX=2016507 GN=speE PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.18GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.86GRR40 pKa = 11.84AKK42 pKa = 10.71LAGG45 pKa = 3.63

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3444

0

3444

1108311

33

2459

321.8

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.524 ± 0.051

0.839 ± 0.012

5.853 ± 0.035

5.785 ± 0.037

2.85 ± 0.025

9.252 ± 0.042

2.158 ± 0.019

3.758 ± 0.029

2.18 ± 0.033

10.4 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.915 ± 0.018

2.101 ± 0.022

5.846 ± 0.036

3.458 ± 0.026

7.893 ± 0.05

5.215 ± 0.025

5.91 ± 0.038

8.576 ± 0.035

1.617 ± 0.022

1.871 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski