Aspergillus fumigatus polymycovirus 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344X3Q4|A0A344X3Q4_9VIRU Methyl transferase OS=Aspergillus fumigatus polymycovirus 1 OX=2250469 PE=4 SV=1
MM1 pKa = 7.93ADD3 pKa = 3.53LTRR6 pKa = 11.84LRR8 pKa = 11.84NIVLSGDD15 pKa = 3.5MKK17 pKa = 10.96VALTAAILYY26 pKa = 8.95HH27 pKa = 6.68LSPNPSAITDD37 pKa = 3.62YY38 pKa = 11.39GVDD41 pKa = 3.46EE42 pKa = 4.7VKK44 pKa = 10.81SVVSWLVSSPGMFYY58 pKa = 11.25GDD60 pKa = 3.27VVAATGITPDD70 pKa = 3.47ILSTFLPALDD80 pKa = 4.73DD81 pKa = 3.81QACADD86 pKa = 3.88AASLANAMATPDD98 pKa = 3.89AEE100 pKa = 4.09WDD102 pKa = 3.55RR103 pKa = 11.84EE104 pKa = 3.94HH105 pKa = 7.63RR106 pKa = 11.84NRR108 pKa = 11.84FTSALAHH115 pKa = 6.41HH116 pKa = 6.59SADD119 pKa = 3.55AARR122 pKa = 11.84LAAQSSAEE130 pKa = 4.03FAAVDD135 pKa = 3.44AMEE138 pKa = 4.19EE139 pKa = 3.78AGYY142 pKa = 10.64EE143 pKa = 4.24NQDD146 pKa = 3.33HH147 pKa = 6.9ARR149 pKa = 11.84TAVNEE154 pKa = 3.91LNRR157 pKa = 11.84VYY159 pKa = 11.09GNTYY163 pKa = 10.04RR164 pKa = 11.84VFTRR168 pKa = 11.84QHH170 pKa = 4.69ARR172 pKa = 11.84SRR174 pKa = 11.84VAIAASFKK182 pKa = 10.37IDD184 pKa = 3.28DD185 pKa = 4.57HH186 pKa = 6.05GTLFHH191 pKa = 7.12VLTPWCLSRR200 pKa = 11.84TSAMIYY206 pKa = 8.32VAVIARR212 pKa = 11.84HH213 pKa = 4.01VRR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84HH218 pKa = 4.78IEE220 pKa = 3.72EE221 pKa = 3.8RR222 pKa = 11.84ASRR225 pKa = 11.84LARR228 pKa = 11.84TALTYY233 pKa = 10.36AARR236 pKa = 11.84AGLEE240 pKa = 3.84AVRR243 pKa = 11.84AIIEE247 pKa = 3.85PAIAIVTSFRR257 pKa = 11.84YY258 pKa = 10.2DD259 pKa = 3.27PAKK262 pKa = 10.33IAFVDD267 pKa = 3.45DD268 pKa = 4.19FDD270 pKa = 4.74RR271 pKa = 11.84VRR273 pKa = 11.84EE274 pKa = 4.3SITTRR279 pKa = 11.84SPPTLVAFACVHH291 pKa = 5.0MCGNQDD297 pKa = 3.66VVSLAARR304 pKa = 11.84LRR306 pKa = 11.84MEE308 pKa = 4.94SLPQLHH314 pKa = 6.03EE315 pKa = 4.11TPPAVVEE322 pKa = 4.32YY323 pKa = 9.52TDD325 pKa = 4.87HH326 pKa = 8.15VDD328 pKa = 4.26DD329 pKa = 4.68EE330 pKa = 4.58QSALLMFSRR339 pKa = 11.84VLKK342 pKa = 10.29RR343 pKa = 11.84LRR345 pKa = 11.84HH346 pKa = 5.08EE347 pKa = 4.55RR348 pKa = 11.84DD349 pKa = 3.2TGVHH353 pKa = 6.36GPRR356 pKa = 11.84LNKK359 pKa = 10.48DD360 pKa = 3.01PMLGYY365 pKa = 9.7IARR368 pKa = 11.84GVRR371 pKa = 11.84RR372 pKa = 11.84SEE374 pKa = 4.22RR375 pKa = 11.84NTMACINRR383 pKa = 11.84VEE385 pKa = 4.19EE386 pKa = 4.08AVSFLRR392 pKa = 11.84SKK394 pKa = 11.16GVDD397 pKa = 3.18TLNTLFVIEE406 pKa = 4.4WGGEE410 pKa = 3.63FDD412 pKa = 4.36YY413 pKa = 9.22PTVMAALAVARR424 pKa = 11.84LDD426 pKa = 3.48VCVDD430 pKa = 3.7IGPSGIDD437 pKa = 4.46LPGADD442 pKa = 4.88AKK444 pKa = 11.41AEE446 pKa = 4.54DD447 pKa = 3.9GTEE450 pKa = 4.02EE451 pKa = 4.13YY452 pKa = 10.7NYY454 pKa = 11.34SLLLASAEE462 pKa = 4.11EE463 pKa = 4.1RR464 pKa = 11.84RR465 pKa = 11.84MPRR468 pKa = 11.84MPRR471 pKa = 11.84VPYY474 pKa = 10.32PSDD477 pKa = 3.37EE478 pKa = 4.09PLEE481 pKa = 4.39AKK483 pKa = 9.9LRR485 pKa = 11.84RR486 pKa = 11.84VAQFYY491 pKa = 10.85SLSGSANIAYY501 pKa = 9.59ISGGVTQNEE510 pKa = 4.22GVPVNICIDD519 pKa = 3.42TANRR523 pKa = 11.84LNAIRR528 pKa = 11.84NVTPDD533 pKa = 3.21VNVVYY538 pKa = 10.74ACAEE542 pKa = 3.88ALLPPLCGHH551 pKa = 6.21GLEE554 pKa = 5.3LGPDD558 pKa = 4.15DD559 pKa = 4.86YY560 pKa = 11.36LQCASLVDD568 pKa = 4.4EE569 pKa = 5.57DD570 pKa = 5.53CPACNAHH577 pKa = 5.81YY578 pKa = 10.11RR579 pKa = 11.84ATAVLSAACADD590 pKa = 3.86RR591 pKa = 11.84QVRR594 pKa = 11.84IVKK597 pKa = 9.38PRR599 pKa = 11.84SAYY602 pKa = 9.64AHH604 pKa = 6.42NGHH607 pKa = 7.18LSLEE611 pKa = 4.41LVPGHH616 pKa = 6.04PTCVDD621 pKa = 3.39DD622 pKa = 4.71TLLTVDD628 pKa = 4.4SMVAANVLRR637 pKa = 11.84NHH639 pKa = 7.01DD640 pKa = 3.84WGDD643 pKa = 3.69EE644 pKa = 4.1PVTPPAGGNPTSPTLARR661 pKa = 11.84VTAEE665 pKa = 3.69NMRR668 pKa = 11.84TVYY671 pKa = 10.58SALNNGAVGPFDD683 pKa = 4.64YY684 pKa = 11.32DD685 pKa = 3.72DD686 pKa = 3.99MASIAASIAPP696 pKa = 3.85

Molecular weight: 75.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344X3Q5|A0A344X3Q5_9VIRU PAS-rp OS=Aspergillus fumigatus polymycovirus 1 OX=2250469 PE=4 SV=1
MM1 pKa = 7.49SPAPILSAEE10 pKa = 4.21QAAHH14 pKa = 7.15LAQLTVDD21 pKa = 4.28DD22 pKa = 4.29VSLVIKK28 pKa = 10.25LASLGLKK35 pKa = 10.34AKK37 pKa = 10.34DD38 pKa = 3.26IQQYY42 pKa = 9.52AAEE45 pKa = 4.24IAEE48 pKa = 4.66GGDD51 pKa = 3.69PEE53 pKa = 5.53IDD55 pKa = 3.66LAPGAPRR62 pKa = 11.84PVVIHH67 pKa = 6.35AWSFVKK73 pKa = 10.56DD74 pKa = 3.22RR75 pKa = 11.84GQYY78 pKa = 9.4HH79 pKa = 5.04STYY82 pKa = 10.41GLSSAQAGEE91 pKa = 4.35LLDD94 pKa = 5.6LLHH97 pKa = 7.12TDD99 pKa = 3.57PKK101 pKa = 10.96DD102 pKa = 3.34AVKK105 pKa = 10.53RR106 pKa = 11.84IQALVSEE113 pKa = 4.92RR114 pKa = 11.84LRR116 pKa = 11.84LRR118 pKa = 11.84GSSRR122 pKa = 11.84PVVVDD127 pKa = 3.86LEE129 pKa = 4.61GAPSSTPAKK138 pKa = 10.26GGAPPSGDD146 pKa = 3.0GGLSLLRR153 pKa = 11.84QEE155 pKa = 4.75IKK157 pKa = 10.56RR158 pKa = 11.84NSGVYY163 pKa = 10.16GSYY166 pKa = 10.46QFTAYY171 pKa = 7.23PTNRR175 pKa = 11.84PGGQAFAVGLGGGLYY190 pKa = 10.72AFGQSKK196 pKa = 10.19AVAVAAARR204 pKa = 11.84IARR207 pKa = 11.84VKK209 pKa = 10.56GRR211 pKa = 11.84SDD213 pKa = 3.61DD214 pKa = 3.55LVRR217 pKa = 11.84PYY219 pKa = 10.62IHH221 pKa = 6.65FWVEE225 pKa = 3.93GSSPRR230 pKa = 11.84AASDD234 pKa = 3.15IPAKK238 pKa = 10.57VWDD241 pKa = 4.34GRR243 pKa = 11.84LGPHH247 pKa = 5.99TEE249 pKa = 4.34PPAEE253 pKa = 4.33PATKK257 pKa = 10.28AKK259 pKa = 10.11TEE261 pKa = 4.08GPTTSGGRR269 pKa = 11.84ASTPKK274 pKa = 10.8GGDD277 pKa = 3.07AAA279 pKa = 4.65

Molecular weight: 29.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2360

279

763

590.0

63.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.907 ± 0.926

1.186 ± 0.311

6.737 ± 0.19

4.449 ± 0.404

3.305 ± 0.459

7.119 ± 0.906

3.008 ± 0.204

3.771 ± 0.238

3.093 ± 0.631

9.153 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.864 ± 0.343

2.542 ± 0.586

6.78 ± 0.496

2.5 ± 0.351

7.415 ± 0.297

7.373 ± 0.395

5.932 ± 0.383

8.22 ± 0.389

0.763 ± 0.063

2.881 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski