Eubacterium sp. CAG:603

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5NL38|R5NL38_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:603 OX=1262891 GN=BN730_00010 PE=4 SV=1
MM1 pKa = 7.75IIDD4 pKa = 3.97EE5 pKa = 4.27EE6 pKa = 4.48KK7 pKa = 9.57EE8 pKa = 4.26YY9 pKa = 11.35CVMVYY14 pKa = 10.37PDD16 pKa = 4.36GDD18 pKa = 3.79EE19 pKa = 4.94DD20 pKa = 4.13TDD22 pKa = 3.85KK23 pKa = 11.55DD24 pKa = 4.02GYY26 pKa = 9.38YY27 pKa = 10.65DD28 pKa = 3.63SYY30 pKa = 11.64KK31 pKa = 10.96VGVDD35 pKa = 4.59DD36 pKa = 4.86YY37 pKa = 10.87MCTLVEE43 pKa = 5.17DD44 pKa = 4.3NIKK47 pKa = 10.73YY48 pKa = 10.79DD49 pKa = 3.78LDD51 pKa = 3.83EE52 pKa = 4.68VIKK55 pKa = 10.83KK56 pKa = 10.18IGIKK60 pKa = 9.53QFISGVYY67 pKa = 8.8VRR69 pKa = 11.84EE70 pKa = 3.82LTGYY74 pKa = 9.36EE75 pKa = 3.82NAIGFSSDD83 pKa = 3.11FYY85 pKa = 11.62LNEE88 pKa = 4.05EE89 pKa = 4.14NNTLVGLLKK98 pKa = 11.18SNDD101 pKa = 3.11VKK103 pKa = 10.67ISYY106 pKa = 10.11NIEE109 pKa = 3.48IPQCEE114 pKa = 4.29FSTNLEE120 pKa = 4.08DD121 pKa = 5.54EE122 pKa = 4.4ITGEE126 pKa = 3.92LKK128 pKa = 10.5KK129 pKa = 11.04YY130 pKa = 9.79ISEE133 pKa = 4.63DD134 pKa = 3.16IIYY137 pKa = 10.74VNIDD141 pKa = 3.3SYY143 pKa = 12.15DD144 pKa = 3.6EE145 pKa = 3.99NTYY148 pKa = 10.01SLRR151 pKa = 11.84KK152 pKa = 9.66KK153 pKa = 9.82IYY155 pKa = 10.3EE156 pKa = 3.78EE157 pKa = 3.83FGYY160 pKa = 10.94DD161 pKa = 3.19KK162 pKa = 11.21LGVLVGIDD170 pKa = 3.44NISFFIEE177 pKa = 4.11EE178 pKa = 4.21EE179 pKa = 4.37TNEE182 pKa = 4.24SKK184 pKa = 11.14

Molecular weight: 21.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5NLK5|R5NLK5_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:603 OX=1262891 GN=BN730_01339 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.33VHH16 pKa = 6.24GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTASGRR28 pKa = 11.84KK29 pKa = 8.42VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2287

0

2287

737511

29

3784

322.5

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.206 ± 0.046

1.347 ± 0.019

6.32 ± 0.046

7.467 ± 0.05

3.971 ± 0.033

6.393 ± 0.045

1.352 ± 0.017

9.177 ± 0.059

8.243 ± 0.047

8.087 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.028

6.192 ± 0.048

2.582 ± 0.025

2.351 ± 0.025

3.635 ± 0.034

6.202 ± 0.048

5.42 ± 0.05

6.753 ± 0.04

0.677 ± 0.015

4.627 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski