Enterobacteria phage C-1 INW-2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Cunavirus; Cunavirus pretoriense

Average proteome isoelectric point is 8.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6X3H8|I6X3H8_9VIRU Putative maturation/attachment protein OS=Enterobacteria phage C-1 INW-2012 OX=1206313 PE=4 SV=1
MM1 pKa = 7.58PQLQTLVLKK10 pKa = 10.76DD11 pKa = 3.68RR12 pKa = 11.84AATPVNHH19 pKa = 6.52TFAPRR24 pKa = 11.84DD25 pKa = 3.25ITNNVGKK32 pKa = 10.27VVEE35 pKa = 4.37SSGVPVGEE43 pKa = 3.86KK44 pKa = 9.73TYY46 pKa = 10.02TISVRR51 pKa = 11.84QTPEE55 pKa = 3.08NGRR58 pKa = 11.84FRR60 pKa = 11.84VQLRR64 pKa = 11.84MAVPTVQNQDD74 pKa = 2.47VGGIINPIVTRR85 pKa = 11.84TAYY88 pKa = 10.65VDD90 pKa = 3.13ATFTFDD96 pKa = 3.12RR97 pKa = 11.84TSTEE101 pKa = 3.75QEE103 pKa = 3.77RR104 pKa = 11.84KK105 pKa = 10.31DD106 pKa = 3.48IVGMFQDD113 pKa = 4.29SLDD116 pKa = 3.7PSKK119 pKa = 11.45ALVNGALINLEE130 pKa = 3.9GVYY133 pKa = 10.62

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6XI01|I6XI01_9VIRU Lysis protein OS=Enterobacteria phage C-1 INW-2012 OX=1206313 PE=4 SV=1
MM1 pKa = 7.83PSLTLRR7 pKa = 11.84VSTNLPDD14 pKa = 3.14ITVRR18 pKa = 11.84DD19 pKa = 4.7AIHH22 pKa = 7.11KK23 pKa = 10.01GYY25 pKa = 10.6LWLRR29 pKa = 11.84KK30 pKa = 8.78QVAVATVWLLILTFIGLSSGKK51 pKa = 9.85LRR53 pKa = 11.84LWFDD57 pKa = 3.77LSVTNLSGG65 pKa = 3.31

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1126

65

522

281.5

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.95 ± 0.768

0.977 ± 0.371

5.151 ± 0.69

5.062 ± 0.982

4.618 ± 0.339

6.75 ± 0.175

1.865 ± 0.263

4.796 ± 1.053

5.151 ± 0.413

10.213 ± 1.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.51 ± 0.225

4.529 ± 0.263

4.796 ± 0.285

2.753 ± 0.679

8.171 ± 0.484

7.549 ± 0.597

7.46 ± 1.007

8.082 ± 1.325

1.865 ± 0.352

2.753 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski