Mycobacterium phage Paito

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Liefievirus; unclassified Liefievirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KJM4|A0A386KJM4_9CAUD Minor tail protein OS=Mycobacterium phage Paito OX=2315544 GN=18 PE=4 SV=1
MM1 pKa = 7.63ALIQDD6 pKa = 3.98QPEE9 pKa = 4.37DD10 pKa = 3.96LPTTNALDD18 pKa = 4.79AIEE21 pKa = 5.76DD22 pKa = 3.94PDD24 pKa = 4.12AEE26 pKa = 4.96PIGEE30 pKa = 4.11RR31 pKa = 11.84AEE33 pKa = 4.36VYY35 pKa = 10.71LSGAHH40 pKa = 6.61VATLDD45 pKa = 3.76NAPTGAGARR54 pKa = 11.84VTLMVEE60 pKa = 4.08LEE62 pKa = 4.34VVEE65 pKa = 4.9EE66 pKa = 3.88GHH68 pKa = 6.67KK69 pKa = 10.36YY70 pKa = 10.07KK71 pKa = 11.01EE72 pKa = 4.1NGEE75 pKa = 4.21VEE77 pKa = 4.31VPIRR81 pKa = 11.84RR82 pKa = 11.84CKK84 pKa = 10.56RR85 pKa = 11.84IGDD88 pKa = 3.33MWRR91 pKa = 11.84PGTEE95 pKa = 3.6RR96 pKa = 11.84PPTKK100 pKa = 10.5EE101 pKa = 3.83EE102 pKa = 3.58IAAQQAAAKK111 pKa = 9.75ARR113 pKa = 11.84AAAEE117 pKa = 3.64QAARR121 pKa = 11.84EE122 pKa = 4.06EE123 pKa = 4.42AEE125 pKa = 4.24RR126 pKa = 11.84AEE128 pKa = 4.27QEE130 pKa = 4.3HH131 pKa = 6.51NEE133 pKa = 3.95PPMFDD138 pKa = 3.57EE139 pKa = 5.2DD140 pKa = 4.56GEE142 pKa = 4.76PISDD146 pKa = 4.07DD147 pKa = 3.58APRR150 pKa = 11.84PPDD153 pKa = 4.26DD154 pKa = 4.68DD155 pKa = 4.52VVDD158 pKa = 4.18AEE160 pKa = 4.47VVEE163 pKa = 5.47DD164 pKa = 5.68DD165 pKa = 6.45DD166 pKa = 4.99EE167 pKa = 6.95ADD169 pKa = 3.91PNTQFDD175 pKa = 3.48VDD177 pKa = 3.71EE178 pKa = 5.23DD179 pKa = 4.02QDD181 pKa = 3.69VSNVVQFSDD190 pKa = 3.47GGKK193 pKa = 9.97

Molecular weight: 21.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KJN3|A0A386KJN3_9CAUD Lysin B OS=Mycobacterium phage Paito OX=2315544 GN=28 PE=4 SV=1
MM1 pKa = 7.23SSYY4 pKa = 10.24RR5 pKa = 11.84VEE7 pKa = 4.35IEE9 pKa = 3.64TSAAKK14 pKa = 9.98QIQRR18 pKa = 11.84LQRR21 pKa = 11.84GEE23 pKa = 4.0QKK25 pKa = 10.55RR26 pKa = 11.84VMVAITALADD36 pKa = 3.4EE37 pKa = 5.05PRR39 pKa = 11.84PHH41 pKa = 7.12GCTKK45 pKa = 10.92LSGTTDD51 pKa = 3.25AYY53 pKa = 10.48RR54 pKa = 11.84IRR56 pKa = 11.84VGNFRR61 pKa = 11.84VVYY64 pKa = 10.62VIDD67 pKa = 5.16DD68 pKa = 3.77GLHH71 pKa = 5.33IVNVTRR77 pKa = 11.84VGHH80 pKa = 5.57RR81 pKa = 11.84RR82 pKa = 11.84EE83 pKa = 4.0VYY85 pKa = 10.06KK86 pKa = 10.83RR87 pKa = 3.3

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13795

36

1342

202.9

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.15 ± 0.584

0.863 ± 0.146

6.981 ± 0.311

5.698 ± 0.445

2.907 ± 0.198

8.996 ± 0.601

2.16 ± 0.244

4.625 ± 0.269

3.639 ± 0.244

7.343 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.114

2.95 ± 0.166

6.118 ± 0.335

3.53 ± 0.185

7.162 ± 0.443

5.132 ± 0.333

6.125 ± 0.291

6.568 ± 0.23

1.827 ± 0.166

1.95 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski