Klebsiella phage vB_KpnM_GF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Jiaodavirus; unclassified Jiaodavirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B7LF76|A0A5B7LF76_9CAUD Inhibitor of prohead protease OS=Klebsiella phage vB_KpnM_GF OX=2510473 PE=4 SV=1
MM1 pKa = 8.16IEE3 pKa = 3.9IYY5 pKa = 10.53GIPEE9 pKa = 4.09EE10 pKa = 4.34VWRR13 pKa = 11.84CPGCKK18 pKa = 9.33AVRR21 pKa = 11.84DD22 pKa = 4.08LLDD25 pKa = 4.02KK26 pKa = 11.12LQLPYY31 pKa = 11.03EE32 pKa = 4.54FYY34 pKa = 11.4NVINEE39 pKa = 3.88VDD41 pKa = 3.73GQPVYY46 pKa = 10.81DD47 pKa = 3.84RR48 pKa = 11.84PLIEE52 pKa = 4.74SLAKK56 pKa = 10.41RR57 pKa = 11.84IGCYY61 pKa = 9.18PSLAIRR67 pKa = 11.84YY68 pKa = 7.51PVIFMDD74 pKa = 4.37NVKK77 pKa = 10.49QYY79 pKa = 10.59DD80 pKa = 3.59IPTFKK85 pKa = 11.12ANLIAAGYY93 pKa = 10.02DD94 pKa = 3.24PDD96 pKa = 5.49IIEE99 pKa = 4.72DD100 pKa = 3.49

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7LF29|A0A5B7LF29_9CAUD NAD(+)--arginine ADP-ribosyltransferase OS=Klebsiella phage vB_KpnM_GF OX=2510473 GN=alt PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 5.44AVFGLIILFFIYY14 pKa = 10.21FLPTFVACSRR24 pKa = 11.84KK25 pKa = 8.85HH26 pKa = 5.83KK27 pKa = 10.54SRR29 pKa = 11.84GGIFITNLVFGWSIIGWLIALIWSASNAQQNTIIIQQVKK68 pKa = 9.73

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

22502

56

748

236.9

26.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.084 ± 0.234

1.089 ± 0.1

6.599 ± 0.161

6.906 ± 0.208

4.12 ± 0.142

6.537 ± 0.213

1.818 ± 0.097

7.088 ± 0.192

7.715 ± 0.309

7.764 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.12

5.346 ± 0.166

3.569 ± 0.15

3.475 ± 0.139

4.626 ± 0.143

5.999 ± 0.164

5.577 ± 0.266

6.248 ± 0.181

1.458 ± 0.072

4.08 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski