Mycobacterium sp. ACS4331

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A1Y0W3|A0A1A1Y0W3_9MYCO 50S ribosomal protein L21 OS=Mycobacterium sp. ACS4331 OX=1834121 GN=rplU PE=3 SV=1
MM1 pKa = 7.11PTEE4 pKa = 4.52APLDD8 pKa = 3.88LVLLAWTRR16 pKa = 11.84RR17 pKa = 11.84QNALTAAGVTRR28 pKa = 11.84ATDD31 pKa = 2.93THH33 pKa = 6.02QRR35 pKa = 11.84GQIAEE40 pKa = 4.28QINSIAMLSTAQAAAQLANAYY61 pKa = 9.44VAEE64 pKa = 5.21DD65 pKa = 4.09DD66 pKa = 4.77PEE68 pKa = 5.57LGNIGSGNVGGGNQGSGNTGSGNTGNNNGGDD99 pKa = 4.0GNIGDD104 pKa = 4.3GNAGNGNQGTGNAGDD119 pKa = 4.16GNTGNNNDD127 pKa = 3.6GNGNTGSGNIGDD139 pKa = 4.33GNQGGNNQGHH149 pKa = 6.75GNTGNANVGDD159 pKa = 4.18GNSGNGNAGDD169 pKa = 4.07GNTGVGNAGDD179 pKa = 4.3GNTGTGNAGDD189 pKa = 4.43GNTGDD194 pKa = 4.43SNPGSGNQGAGNVGDD209 pKa = 4.33GNQGGDD215 pKa = 3.54NQGHH219 pKa = 6.59GNTGNANVGDD229 pKa = 4.32GNSGTGNVGDD239 pKa = 4.31GNTGNGNAGDD249 pKa = 4.18GNQGDD254 pKa = 4.77DD255 pKa = 3.7NVGDD259 pKa = 4.22GNSGTGNTGHH269 pKa = 6.86GNQGSTNAGDD279 pKa = 4.71GNIGDD284 pKa = 4.36NNAGSGNAGDD294 pKa = 5.11LNTGSGNAGSGNSGSGNSGSGNTGDD319 pKa = 4.99GNQGHH324 pKa = 6.49NNAGDD329 pKa = 3.91GNTGTGNAGDD339 pKa = 4.36GNIGDD344 pKa = 4.86HH345 pKa = 5.77NTGSGNDD352 pKa = 3.54GDD354 pKa = 5.18GNTGHH359 pKa = 7.03GNTGNTNTGHH369 pKa = 6.27GNSGSGNQGDD379 pKa = 5.03GNIGDD384 pKa = 4.0NNLPGAHH391 pKa = 6.75GNTGDD396 pKa = 4.75DD397 pKa = 3.58NTGSGNRR404 pKa = 11.84GDD406 pKa = 5.43DD407 pKa = 3.46NTGSGNIGDD416 pKa = 4.36GNGGDD421 pKa = 4.57GNIGDD426 pKa = 4.92DD427 pKa = 3.74NSGSGNIGDD436 pKa = 4.58HH437 pKa = 6.45NSGSGNQGSGNSGGGNRR454 pKa = 11.84GDD456 pKa = 4.37DD457 pKa = 3.53NSGSGNIGDD466 pKa = 4.7HH467 pKa = 5.47NTGHH471 pKa = 6.88GNQGDD476 pKa = 4.08NNSGSGNSGTGLGGGDD492 pKa = 3.61NNSGSGNQGSGNSGSGNIGNDD513 pKa = 3.22NTGHH517 pKa = 6.77ANQGGDD523 pKa = 3.23NSGHH527 pKa = 6.72ANIGDD532 pKa = 3.81NNQGSGNIGSGNSGSGNIGSGNQGSGNIGADD563 pKa = 3.32NQGGGNTGNANPGWGNIGNNNIGLGNKK590 pKa = 9.17GNNNVGFGNTGNNNFGIGLVGDD612 pKa = 3.78NLIGIGGFNSGWFNLGLGNAGNFNIGFGNSGVGNIGFGNSGNYY655 pKa = 10.25NIGFFNSGDD664 pKa = 3.71GNVGFWNSGSGNTGFWNSGKK684 pKa = 10.71ANTGFYY690 pKa = 10.97NSGNTNTGFYY700 pKa = 10.93NSGNTNTGFWNSGDD714 pKa = 3.73TNTGFWNSGNTNTGCYY730 pKa = 10.18NSGDD734 pKa = 4.05TNTGCWNSGNTNTGFSNSGNTNTGKK759 pKa = 10.15HH760 pKa = 5.29NSGGTNTGNYY770 pKa = 8.69NAGGTNTGHH779 pKa = 6.76HH780 pKa = 6.13NAGGTNTGSYY790 pKa = 10.0NAGGTNTGNRR800 pKa = 11.84NAGGTNYY807 pKa = 10.61GDD809 pKa = 3.88YY810 pKa = 11.23NSGGTNTGDD819 pKa = 3.66YY820 pKa = 11.11NSGGTNTGSYY830 pKa = 10.89NSGGTNTGSYY840 pKa = 10.89NSGGTNTGDD849 pKa = 3.66YY850 pKa = 11.11NSGGTNTGHH859 pKa = 6.08YY860 pKa = 10.35NSGGTNTGDD869 pKa = 3.66YY870 pKa = 11.11NSGGTNTGHH879 pKa = 7.34DD880 pKa = 3.7NSGSTNTGNFNAGNTNTGNLNSGNTNTGHH909 pKa = 7.05LNSGDD914 pKa = 3.91TNTGDD919 pKa = 4.0LNSGNTNTGNANSGNTNRR937 pKa = 11.84CDD939 pKa = 3.54HH940 pKa = 6.58NSGNTNTGDD949 pKa = 3.66RR950 pKa = 11.84NSGDD954 pKa = 3.9TNTGNYY960 pKa = 10.08NSGSGNIGDD969 pKa = 4.58HH970 pKa = 6.67NSGSGNNGDD979 pKa = 3.87TNTGHH984 pKa = 7.37RR985 pKa = 11.84KK986 pKa = 7.79TGG988 pKa = 3.24

Molecular weight: 93.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A1WU81|A0A1A1WU81_9MYCO ATP synthase subunit alpha OS=Mycobacterium sp. ACS4331 OX=1834121 GN=atpA PE=3 SV=1
MM1 pKa = 7.3AQHH4 pKa = 6.38KK5 pKa = 10.27RR6 pKa = 11.84NLAKK10 pKa = 10.31RR11 pKa = 11.84VGHH14 pKa = 6.6RR15 pKa = 11.84SSAGPVGLGLGTPLHH30 pKa = 6.13SVEE33 pKa = 4.83NVATSPPVAHH43 pKa = 7.6DD44 pKa = 3.63STSVWGRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84VLLLNSTYY61 pKa = 10.82EE62 pKa = 4.11PLTALPMRR70 pKa = 11.84RR71 pKa = 11.84AIVMLLCGKK80 pKa = 10.53ADD82 pKa = 3.62VVHH85 pKa = 7.72DD86 pKa = 4.63DD87 pKa = 3.59PNGPVIHH94 pKa = 6.52SATRR98 pKa = 11.84AIAVPSVIRR107 pKa = 11.84LRR109 pKa = 11.84SYY111 pKa = 10.66VRR113 pKa = 11.84VPYY116 pKa = 10.05RR117 pKa = 11.84ARR119 pKa = 11.84VPMTRR124 pKa = 11.84AALMHH129 pKa = 6.99RR130 pKa = 11.84DD131 pKa = 3.56RR132 pKa = 11.84FRR134 pKa = 11.84CAYY137 pKa = 9.43CGSKK141 pKa = 10.56ADD143 pKa = 4.08TVDD146 pKa = 3.26HH147 pKa = 5.51VVPRR151 pKa = 11.84SRR153 pKa = 11.84GGEE156 pKa = 4.13HH157 pKa = 4.92TWEE160 pKa = 4.1NCVACCSTCNHH171 pKa = 6.33RR172 pKa = 11.84KK173 pKa = 9.55ADD175 pKa = 4.13RR176 pKa = 11.84LLSEE180 pKa = 5.25LGWSLRR186 pKa = 11.84RR187 pKa = 11.84APMPPKK193 pKa = 8.9GQHH196 pKa = 4.68WRR198 pKa = 11.84LLSTVKK204 pKa = 10.41EE205 pKa = 4.26LDD207 pKa = 4.0PAWVRR212 pKa = 11.84YY213 pKa = 9.55LGEE216 pKa = 4.14GAAA219 pKa = 4.63

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5193

0

5193

1739715

37

6600

335.0

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.06 ± 0.047

0.8 ± 0.008

6.405 ± 0.025

5.364 ± 0.031

3.075 ± 0.019

8.942 ± 0.051

2.202 ± 0.014

4.211 ± 0.022

2.036 ± 0.024

9.838 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.013

2.207 ± 0.023

5.815 ± 0.031

2.941 ± 0.015

7.177 ± 0.034

5.369 ± 0.019

6.102 ± 0.024

8.878 ± 0.034

1.482 ± 0.016

2.066 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski