Burkholderia phage AP3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Kisquinquevirus; Burkholderia virus AP3

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5NR72|A0A1S5NR72_9CAUD Putative baseplate assembly protein OS=Burkholderia phage AP3 OX=1636201 GN=vB_BceM_AP3_0023 PE=4 SV=1
MM1 pKa = 7.81MIRR4 pKa = 11.84ALQGEE9 pKa = 4.8TVDD12 pKa = 3.74ALCWRR17 pKa = 11.84VLGRR21 pKa = 11.84TRR23 pKa = 11.84GVVEE27 pKa = 5.05AVLDD31 pKa = 4.17LNRR34 pKa = 11.84DD35 pKa = 3.4LAQYY39 pKa = 10.96GPILPHH45 pKa = 6.42GLLVEE50 pKa = 4.89LPDD53 pKa = 4.34EE54 pKa = 4.68VPQAAQSGAEE64 pKa = 4.6RR65 pKa = 11.84LQLWDD70 pKa = 3.27

Molecular weight: 7.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7AE31|A0A1U7AE31_9CAUD O-spanin OS=Burkholderia phage AP3 OX=1636201 GN=vB_BceM_AP3_0017 PE=4 SV=1
MM1 pKa = 7.73WIAKK5 pKa = 9.68RR6 pKa = 11.84IAPALFLLALCACTTAPPPTCRR28 pKa = 11.84RR29 pKa = 11.84AIPEE33 pKa = 3.82ALMRR37 pKa = 11.84PPRR40 pKa = 11.84ALHH43 pKa = 6.07SLPSPQPSPATTPPVMKK60 pKa = 10.08TPSNN64 pKa = 3.62

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11554

39

1113

226.5

24.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.255 ± 0.637

0.926 ± 0.129

6.405 ± 0.433

4.933 ± 0.322

3.306 ± 0.2

8.006 ± 0.624

1.999 ± 0.218

4.509 ± 0.196

4.232 ± 0.271

8.75 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.158

3.185 ± 0.358

4.778 ± 0.358

3.748 ± 0.214

8.317 ± 0.463

5.08 ± 0.191

6.007 ± 0.307

7.019 ± 0.272

1.636 ± 0.148

2.605 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski