Maribacter sp. RZ26

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Maribacter; unclassified Maribacter

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4327 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S3PTN6|A0A5S3PTN6_9FLAO Uncharacterized protein OS=Maribacter sp. RZ26 OX=2578118 GN=FEE95_12415 PE=4 SV=1
MM1 pKa = 7.81NSIQPSKK8 pKa = 10.58FPQNNMDD15 pKa = 4.86RR16 pKa = 11.84ILLYY20 pKa = 10.79FMLMASFMATSQTTFIEE37 pKa = 4.45SATAHH42 pKa = 6.34GLNISGNKK50 pKa = 9.86DD51 pKa = 3.09GGHH54 pKa = 6.54AWSDD58 pKa = 3.42FDD60 pKa = 5.89RR61 pKa = 11.84DD62 pKa = 2.99GDD64 pKa = 4.17LDD66 pKa = 4.01VLVLEE71 pKa = 4.56NTNSKK76 pKa = 10.63SYY78 pKa = 10.99LMRR81 pKa = 11.84NNSGTYY87 pKa = 10.55SNVQATLAPGMLSSAAEE104 pKa = 4.04RR105 pKa = 11.84QAVWGDD111 pKa = 3.47LNNDD115 pKa = 3.15GRR117 pKa = 11.84PDD119 pKa = 3.7FMIGSHH125 pKa = 6.5GNTNSNPAFQIFIQNQDD142 pKa = 2.64GSFGDD147 pKa = 3.76GKK149 pKa = 10.97GGYY152 pKa = 7.59TPITVGRR159 pKa = 11.84SGYY162 pKa = 8.69TININPINAEE172 pKa = 3.71GAGFFDD178 pKa = 5.29FEE180 pKa = 5.45GDD182 pKa = 3.18GDD184 pKa = 4.44LDD186 pKa = 4.12IFFDD190 pKa = 3.52SHH192 pKa = 7.59NYY194 pKa = 9.96GIEE197 pKa = 4.0LLRR200 pKa = 11.84NNYY203 pKa = 9.03ISHH206 pKa = 7.02ISHH209 pKa = 7.43TIINPPATSMFTHH222 pKa = 6.57ITTGNGNGVVNFGLNQFASDD242 pKa = 3.7GDD244 pKa = 4.09YY245 pKa = 10.01GTAADD250 pKa = 4.3VDD252 pKa = 3.97DD253 pKa = 5.88DD254 pKa = 4.05GWVDD258 pKa = 2.52IFMRR262 pKa = 11.84KK263 pKa = 9.45RR264 pKa = 11.84NEE266 pKa = 3.49NDD268 pKa = 2.7FFLNQGGVFSNGSDD282 pKa = 3.67LAQAHH287 pKa = 6.06NNNKK291 pKa = 9.54GGNGLWDD298 pKa = 4.35LDD300 pKa = 3.81NDD302 pKa = 4.14GDD304 pKa = 5.06LDD306 pKa = 4.76AVWTEE311 pKa = 3.7NDD313 pKa = 2.88LTQIFRR319 pKa = 11.84NDD321 pKa = 3.46GPGVWTALGATVFPGLPQPSNTNQGNSNASIDD353 pKa = 3.76ALAGGDD359 pKa = 3.72IDD361 pKa = 4.66NDD363 pKa = 3.57GDD365 pKa = 3.55IDD367 pKa = 4.88IILVGNNRR375 pKa = 11.84SYY377 pKa = 11.66LYY379 pKa = 10.2INQLNSPTPAPGVIGSGAAMTFSLDD404 pKa = 3.34PQTFNSSNGEE414 pKa = 3.95GTTMVDD420 pKa = 4.35FDD422 pKa = 6.3DD423 pKa = 6.71DD424 pKa = 4.61GDD426 pKa = 4.26LDD428 pKa = 3.81IYY430 pKa = 10.78MSISGTNRR438 pKa = 11.84LYY440 pKa = 11.34VNQLPVANRR449 pKa = 11.84NNHH452 pKa = 5.36LMVNVSEE459 pKa = 4.89DD460 pKa = 3.45RR461 pKa = 11.84NADD464 pKa = 3.19GSTGGFAGRR473 pKa = 11.84TAIGTNVLIRR483 pKa = 11.84DD484 pKa = 3.77CDD486 pKa = 3.81GNIISGLRR494 pKa = 11.84QVNGVFGHH502 pKa = 6.3GTQQPEE508 pKa = 4.14EE509 pKa = 4.14VHH511 pKa = 6.65FGLPLGEE518 pKa = 4.06NQTYY522 pKa = 10.21LIEE525 pKa = 3.88VHH527 pKa = 6.14YY528 pKa = 10.35PNFYY532 pKa = 11.15DD533 pKa = 5.26PIDD536 pKa = 3.8GFTRR540 pKa = 11.84LIATGIAQPSLIAGTNHH557 pKa = 5.14YY558 pKa = 9.17TLTTTDD564 pKa = 4.45AEE566 pKa = 4.43SLQNEE571 pKa = 4.45NAPVANDD578 pKa = 3.45DD579 pKa = 4.36FEE581 pKa = 4.66TVSYY585 pKa = 8.26GTAVSVQINLFDD597 pKa = 4.66NDD599 pKa = 4.32SEE601 pKa = 4.49PDD603 pKa = 3.45GEE605 pKa = 4.22NFYY608 pKa = 10.64IEE610 pKa = 5.71SIVQPAIGSVTIDD623 pKa = 3.6DD624 pKa = 4.53ADD626 pKa = 3.94NGLVTYY632 pKa = 8.32TYY634 pKa = 10.82SAGIPFPGATFDD646 pKa = 3.86YY647 pKa = 9.52TISDD651 pKa = 3.9AQVTLCPSAGLSDD664 pKa = 3.96SATVTIDD671 pKa = 3.85EE672 pKa = 4.82PCADD676 pKa = 3.9PTGVDD681 pKa = 3.34TDD683 pKa = 3.79GDD685 pKa = 4.56GINDD689 pKa = 3.63VCDD692 pKa = 4.85LDD694 pKa = 5.46DD695 pKa = 6.05DD696 pKa = 4.6NDD698 pKa = 5.07GILDD702 pKa = 3.67TVEE705 pKa = 3.84RR706 pKa = 11.84AKK708 pKa = 10.23TVLWVTQGTPEE719 pKa = 4.1TEE721 pKa = 4.09EE722 pKa = 3.76QNTIDD727 pKa = 4.44KK728 pKa = 8.77LTALGYY734 pKa = 8.66TVTVVDD740 pKa = 5.13DD741 pKa = 4.16NVGGNANDD749 pKa = 3.82FSVTFLYY756 pKa = 10.94EE757 pKa = 4.11DD758 pKa = 4.03VNSGTAYY765 pKa = 10.73ANVANLSTTTNGVITSEE782 pKa = 3.77NALFDD787 pKa = 5.14EE788 pKa = 4.69ILGTSGATGNTNTNFINITDD808 pKa = 3.81NTHH811 pKa = 7.44PITSNLPLGNYY822 pKa = 9.69DD823 pKa = 3.29VGDD826 pKa = 3.35AAFYY830 pKa = 10.44VNNVVSGQKK839 pKa = 10.21LGNHH843 pKa = 6.97PNGQAVLIVWEE854 pKa = 5.09EE855 pKa = 3.8GDD857 pKa = 4.97AMDD860 pKa = 4.82TGIAPGRR867 pKa = 11.84RR868 pKa = 11.84TVLPLTNSTGGFNSNGEE885 pKa = 4.24DD886 pKa = 3.6LLVNAIIWTGNIDD899 pKa = 3.49TDD901 pKa = 3.44EE902 pKa = 5.67DD903 pKa = 4.61GIADD907 pKa = 4.3HH908 pKa = 7.25LDD910 pKa = 3.45SDD912 pKa = 5.08SDD914 pKa = 4.51ADD916 pKa = 3.87GCNDD920 pKa = 4.62AIEE923 pKa = 4.95AYY925 pKa = 10.28SNLSADD931 pKa = 4.13PDD933 pKa = 4.24SNDD936 pKa = 3.27YY937 pKa = 11.13YY938 pKa = 11.14GTGNPPPVNTDD949 pKa = 3.09GTVVAASYY957 pKa = 7.51QTPADD962 pKa = 3.55NDD964 pKa = 4.22SNGTYY969 pKa = 10.58DD970 pKa = 3.52FQEE973 pKa = 4.7AGTAPSISSQPANLTICPGCSGTFSVTASNSDD1005 pKa = 3.32GYY1007 pKa = 10.57QWQTFNGTSWVDD1019 pKa = 3.57LTDD1022 pKa = 3.63SGIHH1026 pKa = 6.24SGTTTSTLNITNATPSDD1043 pKa = 3.43NGNQYY1048 pKa = 10.47RR1049 pKa = 11.84VLVSNSLFICTTIISNTTILTVNVSSVITNRR1080 pKa = 11.84RR1081 pKa = 11.84ITYY1084 pKa = 8.77RR1085 pKa = 11.84VKK1087 pKa = 10.43MNN1089 pKa = 3.57

Molecular weight: 115.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S3PVC9|A0A5S3PVC9_9FLAO Crp/Fnr family transcriptional regulator OS=Maribacter sp. RZ26 OX=2578118 GN=FEE95_05685 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.63LKK7 pKa = 10.48PVTPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.56KK28 pKa = 11.07PEE30 pKa = 4.36KK31 pKa = 10.57SLLAPLKK38 pKa = 10.66KK39 pKa = 10.41SGGRR43 pKa = 11.84NSQGKK48 pKa = 5.92MTIRR52 pKa = 11.84QKK54 pKa = 11.2GGGHH58 pKa = 5.43KK59 pKa = 9.79RR60 pKa = 11.84RR61 pKa = 11.84YY62 pKa = 9.28RR63 pKa = 11.84VIDD66 pKa = 4.1FKK68 pKa = 11.07RR69 pKa = 11.84DD70 pKa = 3.59KK71 pKa = 10.93QDD73 pKa = 2.67VTATVKK79 pKa = 10.61SIQYY83 pKa = 9.95DD84 pKa = 3.58PNRR87 pKa = 11.84TAFIALLEE95 pKa = 4.24YY96 pKa = 10.92VDD98 pKa = 3.68GEE100 pKa = 3.98KK101 pKa = 10.33RR102 pKa = 11.84YY103 pKa = 10.25IIAQNGLQVDD113 pKa = 3.85QKK115 pKa = 9.61VTSGANAAPEE125 pKa = 4.12IGNALPLSAIPLGTIISCIEE145 pKa = 3.75LRR147 pKa = 11.84PGQGAVMARR156 pKa = 11.84SAGTFAQLMAKK167 pKa = 9.93DD168 pKa = 4.08GKK170 pKa = 10.42FVTVKK175 pKa = 9.72MPSGEE180 pKa = 4.12TRR182 pKa = 11.84LILSDD187 pKa = 3.82CLATIGAVSNSDD199 pKa = 3.2HH200 pKa = 6.21QLLVSGKK207 pKa = 9.49AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 3.89GRR238 pKa = 11.84ASGGHH243 pKa = 5.13PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.27GYY255 pKa = 7.48RR256 pKa = 11.84TRR258 pKa = 11.84QKK260 pKa = 9.45TKK262 pKa = 9.43STNKK266 pKa = 10.22YY267 pKa = 8.44ILEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.2KK274 pKa = 9.94

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4327

0

4327

1542337

30

5436

356.4

40.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.471 ± 0.036

0.752 ± 0.015

5.805 ± 0.035

6.811 ± 0.039

5.18 ± 0.029

6.855 ± 0.043

1.765 ± 0.019

7.441 ± 0.037

7.37 ± 0.056

9.293 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.018

5.772 ± 0.039

3.58 ± 0.023

3.296 ± 0.019

3.6 ± 0.023

6.592 ± 0.033

5.76 ± 0.052

6.269 ± 0.029

1.191 ± 0.016

3.919 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski