Bacillus azotoformans LMG 9581

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Schinkia; Schinkia azotoformans

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4207 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6DH86|K6DH86_BACAZ Small acid-soluble spore protein I OS=Bacillus azotoformans LMG 9581 OX=1131731 GN=sspI PE=2 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84QTLVVNFGEE11 pKa = 4.33YY12 pKa = 10.6EE13 pKa = 3.93FTNFEE18 pKa = 4.14RR19 pKa = 11.84AVKK22 pKa = 10.31SLEE25 pKa = 3.82EE26 pKa = 4.05EE27 pKa = 4.0YY28 pKa = 10.73GYY30 pKa = 11.2EE31 pKa = 4.0GFAWDD36 pKa = 4.12MVVASNDD43 pKa = 3.49FEE45 pKa = 4.39ILCEE49 pKa = 3.96FLSDD53 pKa = 4.47DD54 pKa = 5.04GIDD57 pKa = 3.67AEE59 pKa = 5.39IVICC63 pKa = 5.58

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6DKH9|K6DKH9_BACAZ Uncharacterized protein OS=Bacillus azotoformans LMG 9581 OX=1131731 GN=BAZO_03260 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.21QPNNRR10 pKa = 11.84KK11 pKa = 8.85HH12 pKa = 6.37KK13 pKa = 9.52KK14 pKa = 7.68VHH16 pKa = 5.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4207

0

4207

1198567

26

2248

284.9

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.681 ± 0.041

0.767 ± 0.013

5.088 ± 0.032

7.487 ± 0.048

4.593 ± 0.029

6.783 ± 0.037

1.939 ± 0.019

8.448 ± 0.038

7.271 ± 0.039

9.661 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.017

4.823 ± 0.032

3.548 ± 0.025

3.64 ± 0.027

3.881 ± 0.028

5.876 ± 0.029

5.419 ± 0.03

6.942 ± 0.032

0.978 ± 0.014

3.482 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski