Planktothrix tepida PCC 9214

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Planktothrix; Planktothrix tepida

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J1LNU7|A0A1J1LNU7_9CYAN Uncharacterized protein OS=Planktothrix tepida PCC 9214 OX=671072 GN=PL921460027 PE=4 SV=1
MM1 pKa = 7.67PGAVLVGGCNSNGFGDD17 pKa = 3.65GGGVYY22 pKa = 9.5IEE24 pKa = 4.41YY25 pKa = 10.55GEE27 pKa = 4.89KK28 pKa = 10.05IAIDD32 pKa = 3.67FADD35 pKa = 3.98RR36 pKa = 11.84VPWTILARR44 pKa = 11.84EE45 pKa = 3.97QDD47 pKa = 3.63VGVPIRR53 pKa = 11.84VKK55 pKa = 10.58VGCGDD60 pKa = 3.59GTXFTPTPSYY70 pKa = 10.3IGPASFEE77 pKa = 4.22YY78 pKa = 10.15TVNDD82 pKa = 3.81GNGGISTATANITVEE97 pKa = 4.25ALPNQPPILVDD108 pKa = 3.57DD109 pKa = 4.38TATTQQDD116 pKa = 3.35IPVTLTPLNNDD127 pKa = 3.24TXQQ130 pKa = 3.74

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J1LT61|A0A1J1LT61_9CYAN Transcriptional regulator XRE family OS=Planktothrix tepida PCC 9214 OX=671072 GN=PL9214670032 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84IQTAFMNIPNQQRR15 pKa = 11.84WRR17 pKa = 11.84NLFRR21 pKa = 11.84GRR23 pKa = 11.84HH24 pKa = 4.32LARR27 pKa = 11.84YY28 pKa = 6.48TRR30 pKa = 11.84LKK32 pKa = 9.77STLDD36 pKa = 3.3SSFVNLSSFGLSLKK50 pKa = 10.86NPGQPFATTALHH62 pKa = 6.68LRR64 pKa = 11.84SCVVFEE70 pKa = 4.39SLGNVPLQPQDD81 pKa = 3.69YY82 pKa = 9.15FQKK85 pKa = 10.34FCLAIVWRR93 pKa = 11.84LIEE96 pKa = 4.14QYY98 pKa = 10.32TRR100 pKa = 11.84IAGDD104 pKa = 3.33TSS106 pKa = 3.25

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5889

0

5889

1873568

23

3289

318.1

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.923 ± 0.031

1.011 ± 0.011

4.879 ± 0.027

6.529 ± 0.032

4.059 ± 0.021

6.427 ± 0.042

1.84 ± 0.016

7.229 ± 0.03

5.027 ± 0.033

11.286 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.813 ± 0.015

4.727 ± 0.037

4.815 ± 0.029

5.827 ± 0.035

4.626 ± 0.027

6.472 ± 0.029

5.786 ± 0.034

6.095 ± 0.029

1.447 ± 0.014

3.179 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski