Lysinibacillus sp. 2017

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus; unclassified Lysinibacillus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GYJ1|A0A2S1GYJ1_9BACI Putative phosphoesterase DCE79_03305 OS=Lysinibacillus sp. 2017 OX=2169540 GN=DCE79_03305 PE=3 SV=1
MM1 pKa = 7.51GCGRR5 pKa = 11.84GDD7 pKa = 3.65NEE9 pKa = 5.19VGGQTSNRR17 pKa = 11.84GCVCEE22 pKa = 4.11VVRR25 pKa = 11.84AILEE29 pKa = 4.01IQNAAVQDD37 pKa = 3.79EE38 pKa = 4.77CSNCTTNCFLEE49 pKa = 4.16PLGGIVNPARR59 pKa = 11.84SSVDD63 pKa = 2.93TRR65 pKa = 11.84VFTLLTKK72 pKa = 10.68DD73 pKa = 3.59GSPFFAQFSTSDD85 pKa = 3.93GDD87 pKa = 4.12CEE89 pKa = 4.37PCVSIYY95 pKa = 10.53FRR97 pKa = 11.84VEE99 pKa = 3.94DD100 pKa = 4.65VFDD103 pKa = 4.14GCCATLRR110 pKa = 11.84VLVPLDD116 pKa = 3.88EE117 pKa = 5.78NEE119 pKa = 5.05DD120 pKa = 3.93PVDD123 pKa = 4.26LLNDD127 pKa = 4.24DD128 pKa = 3.72GTKK131 pKa = 9.57ISLRR135 pKa = 11.84EE136 pKa = 3.95VCKK139 pKa = 9.54VTQFATSEE147 pKa = 4.27SCVTVDD153 pKa = 5.12LDD155 pKa = 4.04CFCAVQCIDD164 pKa = 5.01DD165 pKa = 4.12VDD167 pKa = 5.37LGICNN172 pKa = 3.83

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1H6B8|A0A2S1H6B8_9BACI Aldehyde dehydrogenase OS=Lysinibacillus sp. 2017 OX=2169540 GN=DCE79_18080 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.36KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3417

0

3417

1001123

17

4133

293.0

32.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.479 ± 0.049

0.674 ± 0.014

4.98 ± 0.033

7.415 ± 0.049

4.658 ± 0.035

6.445 ± 0.042

1.989 ± 0.024

8.113 ± 0.046

6.652 ± 0.041

9.695 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.021

4.635 ± 0.038

3.349 ± 0.026

4.068 ± 0.033

3.704 ± 0.031

5.849 ± 0.031

5.844 ± 0.054

7.152 ± 0.036

0.895 ± 0.014

3.602 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski