Erysipelotrichaceae bacterium OH741_COT-311

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; unclassified Erysipelotrichaceae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1SAJ6|A0A3P1SAJ6_9FIRM Asp23/Gls24 family envelope stress response protein OS=Erysipelotrichaceae bacterium OH741_COT-311 OX=2491058 GN=EII25_00425 PE=3 SV=1
MM1 pKa = 7.45IKK3 pKa = 10.09VVYY6 pKa = 9.32WSSTGNTEE14 pKa = 4.1QMAEE18 pKa = 4.32SIVKK22 pKa = 10.08GIQEE26 pKa = 4.14AGKK29 pKa = 9.76EE30 pKa = 3.98AEE32 pKa = 4.76LLNLSDD38 pKa = 4.88ISVDD42 pKa = 3.65DD43 pKa = 4.33VKK45 pKa = 11.55DD46 pKa = 3.5EE47 pKa = 4.46DD48 pKa = 4.72VFALGCPAMGAEE60 pKa = 3.95NLEE63 pKa = 4.29EE64 pKa = 5.15GEE66 pKa = 4.25VEE68 pKa = 4.11PFVTEE73 pKa = 4.25LEE75 pKa = 4.12QYY77 pKa = 10.9AAGKK81 pKa = 8.61TILLFGSYY89 pKa = 8.21SWGDD93 pKa = 3.86GQWMRR98 pKa = 11.84DD99 pKa = 3.05WVEE102 pKa = 3.86RR103 pKa = 11.84LANCGANILNNEE115 pKa = 4.0GFICCEE121 pKa = 4.06TPSDD125 pKa = 3.61EE126 pKa = 4.57VLAQGEE132 pKa = 4.32EE133 pKa = 4.17LGKK136 pKa = 10.22QLAQII141 pKa = 4.36

Molecular weight: 15.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1S3U4|A0A3P1S3U4_9FIRM Membrane protein insertase YidC OS=Erysipelotrichaceae bacterium OH741_COT-311 OX=2491058 GN=yidC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.53GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.82TVGGRR28 pKa = 11.84KK29 pKa = 8.6VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84FKK37 pKa = 10.92GRR39 pKa = 11.84KK40 pKa = 5.99TLSAA44 pKa = 4.15

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1436

0

1436

460030

30

2506

320.4

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.771 ± 0.066

1.108 ± 0.023

5.508 ± 0.048

6.791 ± 0.065

4.729 ± 0.054

5.654 ± 0.061

2.214 ± 0.03

8.626 ± 0.061

8.207 ± 0.052

9.865 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.035

5.307 ± 0.051

2.773 ± 0.034

3.723 ± 0.039

3.274 ± 0.043

5.934 ± 0.045

5.119 ± 0.054

7.137 ± 0.053

0.701 ± 0.02

4.84 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski