Sporosarcina newyorkensis 2681

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina; Sporosarcina newyorkensis

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9DQ54|F9DQ54_9BACL Glucose-6-phosphate isomerase (Fragment) OS=Sporosarcina newyorkensis 2681 OX=1027292 GN=pgi3 PE=4 SV=1
VV1 pKa = 7.16LLDD4 pKa = 4.59DD5 pKa = 4.47QDD7 pKa = 4.13HH8 pKa = 7.2DD9 pKa = 4.43SVDD12 pKa = 3.4YY13 pKa = 11.67AMNQLRR19 pKa = 11.84QSFGEE24 pKa = 4.14LFPQVFKK31 pKa = 10.72TITSDD36 pKa = 3.21NGSEE40 pKa = 4.07FSNLTVGLEE49 pKa = 4.28GVTDD53 pKa = 3.85VYY55 pKa = 10.93FCRR58 pKa = 11.84PYY60 pKa = 11.23SPLL63 pKa = 3.52

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9DRN2|F9DRN2_9BACL Hemerythrin HHE cation binding domain protein OS=Sporosarcina newyorkensis 2681 OX=1027292 GN=HMPREF9372_1462 PE=4 SV=1
MM1 pKa = 7.3QKK3 pKa = 11.06DD4 pKa = 3.9EE5 pKa = 4.3MNGRR9 pKa = 11.84ALYY12 pKa = 10.27CSSFLFVNKK21 pKa = 10.25KK22 pKa = 10.15GGQNPFGNRR31 pKa = 11.84LMNKK35 pKa = 8.37GRR37 pKa = 11.84RR38 pKa = 11.84TMDD41 pKa = 3.38CSSYY45 pKa = 10.72IIFTSRR51 pKa = 11.84IAITAAIQLRR61 pKa = 11.84TRR63 pKa = 11.84IGSATAA69 pKa = 3.58

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3825

0

3825

1052618

39

1440

275.2

30.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.044

0.689 ± 0.012

5.181 ± 0.033

7.618 ± 0.048

4.489 ± 0.037

6.909 ± 0.044

2.047 ± 0.017

7.475 ± 0.038

6.358 ± 0.038

9.764 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.02

3.952 ± 0.027

3.666 ± 0.024

3.907 ± 0.031

4.342 ± 0.033

5.978 ± 0.036

5.597 ± 0.023

7.213 ± 0.036

1.004 ± 0.014

3.282 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski