Streptococcus phage IPP63

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SFJ3|A0A1S5SFJ3_9CAUD Nuclease OS=Streptococcus phage IPP63 OX=1916199 GN=IPP63_00028 PE=4 SV=1
MM1 pKa = 7.74VGVTYY6 pKa = 9.81QEE8 pKa = 3.08IHH10 pKa = 6.73LFVEE14 pKa = 5.07FLKK17 pKa = 10.63EE18 pKa = 3.59QYY20 pKa = 10.55GQGRR24 pKa = 11.84PDD26 pKa = 3.75YY27 pKa = 10.97IEE29 pKa = 4.84ALNDD33 pKa = 3.49LDD35 pKa = 4.6GLVEE39 pKa = 4.08VSS41 pKa = 3.4

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SF99|A0A1S5SF99_9CAUD Uncharacterized protein OS=Streptococcus phage IPP63 OX=1916199 GN=IPP63_00020 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.18PYY8 pKa = 10.05SGKK11 pKa = 10.15KK12 pKa = 9.81EE13 pKa = 4.19STFVKK18 pKa = 10.34ADD20 pKa = 3.49PEE22 pKa = 4.22LVEE25 pKa = 4.12KK26 pKa = 10.84LLRR29 pKa = 11.84NTSFLEE35 pKa = 3.92RR36 pKa = 11.84LQKK39 pKa = 10.7KK40 pKa = 9.44PINFQIDD47 pKa = 3.47LLEE50 pKa = 4.82RR51 pKa = 11.84ISRR54 pKa = 11.84LEE56 pKa = 3.87SQVISLANHH65 pKa = 5.71EE66 pKa = 4.41MFKK69 pKa = 10.41IPSSRR74 pKa = 11.84SSTAA78 pKa = 2.78

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10486

38

1852

201.7

22.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.133 ± 0.507

0.525 ± 0.095

5.741 ± 0.393

8.173 ± 0.434

4.387 ± 0.315

5.884 ± 0.318

1.516 ± 0.212

6.857 ± 0.371

8.077 ± 0.309

8.592 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.323

5.77 ± 0.304

2.642 ± 0.264

4.167 ± 0.314

4.883 ± 0.265

6.294 ± 0.481

6.199 ± 0.519

5.97 ± 0.306

1.326 ± 0.136

3.452 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski