Orpheovirus IHUMI-LCC2

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; unclassified Phycodnaviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I2L4T8|A0A2I2L4T8_9PHYC Uncharacterized protein OS=Orpheovirus IHUMI-LCC2 OX=2023057 GN=ORPV_627 PE=4 SV=1
MM1 pKa = 7.86VFEE4 pKa = 5.0GDD6 pKa = 3.61YY7 pKa = 11.11NLEE10 pKa = 4.03FDD12 pKa = 4.74ADD14 pKa = 4.46DD15 pKa = 4.11YY16 pKa = 11.86NIDD19 pKa = 4.61DD20 pKa = 4.73IIMLIMEE27 pKa = 5.01EE28 pKa = 4.22VQDD31 pKa = 4.13SIEE34 pKa = 4.25DD35 pKa = 3.54DD36 pKa = 3.67RR37 pKa = 11.84YY38 pKa = 9.76GTFTYY43 pKa = 10.13EE44 pKa = 3.32ISQPGRR50 pKa = 11.84NKK52 pKa = 9.89VVTHH56 pKa = 5.96VEE58 pKa = 3.97LPRR61 pKa = 11.84DD62 pKa = 3.53NDD64 pKa = 3.9HH65 pKa = 6.96YY66 pKa = 11.92DD67 pKa = 4.35LIQDD71 pKa = 4.11HH72 pKa = 6.69LVATLGNLINDD83 pKa = 3.87NLRR86 pKa = 11.84VRR88 pKa = 11.84LQYY91 pKa = 10.7SVQYY95 pKa = 10.92

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I2L3D9|A0A2I2L3D9_9PHYC UV damage endonuclease UvdE OS=Orpheovirus IHUMI-LCC2 OX=2023057 GN=ORPV_85 PE=4 SV=1
MM1 pKa = 7.6SGIVLIGVGFGVGSVLGYY19 pKa = 10.76LSVLNGFAGALVSGKK34 pKa = 10.2DD35 pKa = 3.09GGGRR39 pKa = 11.84HH40 pKa = 4.54VAKK43 pKa = 10.68GLFLGVLFPVVGTATGYY60 pKa = 10.15LVSRR64 pKa = 11.84FLRR67 pKa = 11.84GG68 pKa = 2.98

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1199

0

1199

325136

50

1433

271.2

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.787 ± 0.062

1.844 ± 0.044

6.574 ± 0.064

5.866 ± 0.072

3.793 ± 0.045

5.16 ± 0.134

1.829 ± 0.037

10.069 ± 0.092

7.727 ± 0.109

9.355 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.036

8.645 ± 0.075

3.066 ± 0.068

2.566 ± 0.077

3.704 ± 0.047

6.634 ± 0.073

4.691 ± 0.071

5.154 ± 0.062

1.054 ± 0.028

6.961 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski