Erwinia phage Cronus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GMD8|A0A2S1GMD8_9CAUD Macro domain protein OS=Erwinia phage Cronus OX=2163633 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.45NQLLEE7 pKa = 4.39DD8 pKa = 4.0MNVEE12 pKa = 4.04PLLAPVIEE20 pKa = 4.33VCYY23 pKa = 9.95ATDD26 pKa = 3.5YY27 pKa = 11.41DD28 pKa = 5.38SYY30 pKa = 11.64DD31 pKa = 2.65VWAPVVQNGEE41 pKa = 3.9DD42 pKa = 3.48QYY44 pKa = 11.29IDD46 pKa = 3.19TGAGVLNLCDD56 pKa = 4.51AIIYY60 pKa = 7.69EE61 pKa = 4.15IRR63 pKa = 11.84IKK65 pKa = 10.66KK66 pKa = 9.43

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GMG4|A0A2S1GMG4_9CAUD Uncharacterized protein OS=Erwinia phage Cronus OX=2163633 PE=4 SV=1
MM1 pKa = 7.29LHH3 pKa = 6.23YY4 pKa = 10.56GIIFLIVALVAAALGFGALAGTAAMAAKK32 pKa = 10.12LVFVVGIIIFLVSLFTGRR50 pKa = 11.84RR51 pKa = 11.84ATTVVV56 pKa = 2.97

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

291

0

291

53896

41

1242

185.2

20.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.9 ± 0.204

0.996 ± 0.057

6.503 ± 0.113

6.809 ± 0.181

4.451 ± 0.099

6.032 ± 0.159

1.714 ± 0.084

6.904 ± 0.116

7.281 ± 0.172

7.746 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.086

5.433 ± 0.119

3.366 ± 0.103

3.451 ± 0.09

4.312 ± 0.091

6.835 ± 0.143

6.095 ± 0.237

6.889 ± 0.128

1.464 ± 0.07

4.145 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski