Rhizobiales bacterium IZ6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; unclassified Hyphomicrobiales

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S6QTZ3|A0A6S6QTZ3_9RHIZ Hemolysin secretion protein D OS=Rhizobiales bacterium IZ6 OX=708113 GN=IZ6_11550 PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.42RR3 pKa = 11.84ILLAGAALAVMTTAPMAADD22 pKa = 3.55YY23 pKa = 9.17AAPADD28 pKa = 3.61VDD30 pKa = 3.49VWSGYY35 pKa = 9.73FVGIQGGYY43 pKa = 9.47AFKK46 pKa = 10.79DD47 pKa = 3.36VEE49 pKa = 4.44TLVDD53 pKa = 3.69GADD56 pKa = 4.27DD57 pKa = 5.03DD58 pKa = 5.24FEE60 pKa = 7.27SDD62 pKa = 3.87DD63 pKa = 4.07FVIGAYY69 pKa = 9.85YY70 pKa = 9.46GRR72 pKa = 11.84NWQSGDD78 pKa = 2.94WVFGLDD84 pKa = 4.44SSISYY89 pKa = 10.18IGLDD93 pKa = 3.33EE94 pKa = 5.86DD95 pKa = 4.75EE96 pKa = 4.55IAGNVNLEE104 pKa = 4.22LEE106 pKa = 4.49ANLLGLSRR114 pKa = 11.84LKK116 pKa = 10.8VGYY119 pKa = 10.34AVDD122 pKa = 3.41NTLFFVAGGVASTYY136 pKa = 10.37FSAEE140 pKa = 3.88DD141 pKa = 3.58TATDD145 pKa = 5.11DD146 pKa = 5.67DD147 pKa = 5.81DD148 pKa = 7.22DD149 pKa = 5.81DD150 pKa = 4.2FALGFTVGAGVEE162 pKa = 4.51HH163 pKa = 7.08KK164 pKa = 10.84FSDD167 pKa = 3.33SWSARR172 pKa = 11.84IEE174 pKa = 3.77YY175 pKa = 10.0AYY177 pKa = 10.83FNIEE181 pKa = 4.01SDD183 pKa = 4.02DD184 pKa = 3.72MTVNADD190 pKa = 3.94DD191 pKa = 4.63IEE193 pKa = 5.18LEE195 pKa = 4.34VEE197 pKa = 3.91GHH199 pKa = 5.25IVRR202 pKa = 11.84GGIAYY207 pKa = 9.53HH208 pKa = 6.3FF209 pKa = 4.53

Molecular weight: 22.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S6QWV0|A0A6S6QWV0_9RHIZ Glyoxalase OS=Rhizobiales bacterium IZ6 OX=708113 GN=IZ6_17430 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.01VLANRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.81HH37 pKa = 5.14GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.68LSAA44 pKa = 3.99

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3159

0

3159

948505

40

4227

300.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.206 ± 0.06

0.722 ± 0.014

5.703 ± 0.046

6.009 ± 0.042

3.913 ± 0.031

8.56 ± 0.045

1.907 ± 0.022

5.429 ± 0.034

4.188 ± 0.046

9.983 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.021

2.673 ± 0.03

5.217 ± 0.035

2.905 ± 0.021

6.691 ± 0.052

5.473 ± 0.033

5.301 ± 0.034

7.415 ± 0.035

1.228 ± 0.018

2.181 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski