Sediminispirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228) (Spirochaeta smaragdinae)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Sediminispirochaeta; Sediminispirochaeta smaragdinae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1R7Y3|E1R7Y3_SEDSS 30S ribosomal protein S9 OS=Sediminispirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228) OX=573413 GN=rpsI PE=3 SV=1
MM1 pKa = 7.5SSLRR5 pKa = 11.84RR6 pKa = 11.84ITTVAFTAAALLLAGVSLSADD27 pKa = 3.38DD28 pKa = 5.57FSFDD32 pKa = 5.03DD33 pKa = 4.48FGSSDD38 pKa = 3.61FGDD41 pKa = 3.79DD42 pKa = 3.27TGTATAGSSSAIEE55 pKa = 3.9VHH57 pKa = 6.6GEE59 pKa = 3.63LDD61 pKa = 3.18GSLRR65 pKa = 11.84ATVGEE70 pKa = 4.71DD71 pKa = 3.01ALSDD75 pKa = 3.08IGAISSEE82 pKa = 4.15EE83 pKa = 3.71DD84 pKa = 3.31SIEE87 pKa = 4.14YY88 pKa = 10.53GSEE91 pKa = 3.59AKK93 pKa = 10.8LNLTYY98 pKa = 10.65TGDD101 pKa = 3.81TIDD104 pKa = 5.14AFINLQIDD112 pKa = 3.75PEE114 pKa = 4.08RR115 pKa = 11.84LEE117 pKa = 6.12DD118 pKa = 5.05DD119 pKa = 3.2MSTLFDD125 pKa = 3.43EE126 pKa = 5.77AYY128 pKa = 9.22IRR130 pKa = 11.84YY131 pKa = 8.86YY132 pKa = 11.11GSGFDD137 pKa = 5.01FEE139 pKa = 4.66TGLMKK144 pKa = 10.27VVWGKK149 pKa = 10.95GDD151 pKa = 3.38QLHH154 pKa = 6.5VVDD157 pKa = 5.89LLNSDD162 pKa = 4.73DD163 pKa = 4.47LTDD166 pKa = 4.75FVNPDD171 pKa = 3.51YY172 pKa = 10.73IDD174 pKa = 3.52RR175 pKa = 11.84RR176 pKa = 11.84LAVPMAKK183 pKa = 10.3LNVQVGVQGKK193 pKa = 9.84LEE195 pKa = 4.06FVYY198 pKa = 10.58IPTLTPNTNPTSGAWATQQAQDD220 pKa = 4.3LVDD223 pKa = 3.42KK224 pKa = 9.73TEE226 pKa = 3.86EE227 pKa = 4.3YY228 pKa = 8.39ITYY231 pKa = 10.17VALSAYY237 pKa = 10.29NEE239 pKa = 4.61TYY241 pKa = 9.53TATSSEE247 pKa = 4.1AAATLAEE254 pKa = 3.96ASILEE259 pKa = 4.34KK260 pKa = 11.16YY261 pKa = 10.62SDD263 pKa = 3.86GSALYY268 pKa = 9.76PDD270 pKa = 5.17TDD272 pKa = 3.72TLDD275 pKa = 3.49YY276 pKa = 11.07SQAAVRR282 pKa = 11.84WTDD285 pKa = 2.99SFGGIDD291 pKa = 3.75VGALYY296 pKa = 11.07YY297 pKa = 10.77CGFLKK302 pKa = 10.71DD303 pKa = 3.75PSISYY308 pKa = 8.75TVDD311 pKa = 2.94GSGTPTAIDD320 pKa = 3.09ISYY323 pKa = 10.97DD324 pKa = 3.29RR325 pKa = 11.84LHH327 pKa = 7.1AFGLEE332 pKa = 3.69AGGIVGGFNLRR343 pKa = 11.84SEE345 pKa = 4.27AAYY348 pKa = 11.4YY349 pKa = 6.8MTEE352 pKa = 5.04DD353 pKa = 3.44IAGDD357 pKa = 3.75DD358 pKa = 3.59PVVHH362 pKa = 6.48NNSLNYY368 pKa = 10.43LVGFDD373 pKa = 4.92RR374 pKa = 11.84DD375 pKa = 3.86LPLHH379 pKa = 6.32NINLNMQATGSYY391 pKa = 10.03ILNNDD396 pKa = 3.94EE397 pKa = 4.61ISNGDD402 pKa = 3.3IEE404 pKa = 5.17YY405 pKa = 10.87DD406 pKa = 3.51GDD408 pKa = 4.48DD409 pKa = 4.74DD410 pKa = 4.67YY411 pKa = 12.15SSNLIVCKK419 pKa = 10.67ISDD422 pKa = 3.91TFNHH426 pKa = 5.92EE427 pKa = 4.2KK428 pKa = 10.77VSIEE432 pKa = 3.63AKK434 pKa = 10.15GIYY437 pKa = 9.66EE438 pKa = 4.69IEE440 pKa = 4.77DD441 pKa = 4.18EE442 pKa = 4.4DD443 pKa = 3.99WMIGPEE449 pKa = 3.83ITLAPNGEE457 pKa = 4.36FEE459 pKa = 4.55FSAEE463 pKa = 3.66ARR465 pKa = 11.84FFGGDD470 pKa = 3.16DD471 pKa = 4.1DD472 pKa = 5.2GRR474 pKa = 11.84LGQFDD479 pKa = 4.05EE480 pKa = 4.72SSYY483 pKa = 11.41LQFKK487 pKa = 8.9VKK489 pKa = 10.83AMFF492 pKa = 4.09

Molecular weight: 53.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1R3T1|E1R3T1_SEDSS O-acetylhomoserine/O-acetylserine sulfhydrylase OS=Sediminispirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228) OX=573413 GN=Spirs_2949 PE=3 SV=1
MM1 pKa = 7.49VKK3 pKa = 10.35LLCALGLVCLLSLLAGRR20 pKa = 11.84YY21 pKa = 8.01PSAGFTAPSTFIHH34 pKa = 7.29DD35 pKa = 3.61RR36 pKa = 11.84LARR39 pKa = 11.84NLLLSLRR46 pKa = 11.84LPRR49 pKa = 11.84LIAALLLGLALGAGGTVFQMLFSNPLVEE77 pKa = 4.68PGFLGVSQGAAFGAAAAIVLGGTAAWLIQISAACFAFVGLGASYY121 pKa = 11.27LLARR125 pKa = 11.84HH126 pKa = 5.71FRR128 pKa = 11.84FGGWTLRR135 pKa = 11.84LVLAGITVSALFSAGVGILKK155 pKa = 8.36YY156 pKa = 8.9TADD159 pKa = 4.23PMSQLPEE166 pKa = 3.59ITFWLLGGLWSVGWKK181 pKa = 9.62EE182 pKa = 4.08VLSVLPVVLLAALLLFAHH200 pKa = 7.43RR201 pKa = 11.84WRR203 pKa = 11.84LNLLSMDD210 pKa = 4.04DD211 pKa = 3.3RR212 pKa = 11.84TAFSFGIAPVRR223 pKa = 11.84EE224 pKa = 4.0RR225 pKa = 11.84LILLAAATAATAAVISISGMISWVGLIVPHH255 pKa = 6.06MARR258 pKa = 11.84KK259 pKa = 9.53LFSANSRR266 pKa = 11.84WSLPGAMMLGACFTIVCDD284 pKa = 3.68TLARR288 pKa = 11.84TLLAGEE294 pKa = 4.46IPLGVLTSFIGACFFIILLTKK315 pKa = 10.44KK316 pKa = 8.22STKK319 pKa = 9.91RR320 pKa = 11.84GRR322 pKa = 3.36

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4211

0

4211

1432067

31

3165

340.1

37.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.177 ± 0.042

1.026 ± 0.012

5.452 ± 0.032

7.064 ± 0.043

4.606 ± 0.024

7.657 ± 0.035

1.919 ± 0.017

7.154 ± 0.036

5.23 ± 0.033

10.287 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.017

3.402 ± 0.024

4.092 ± 0.024

2.965 ± 0.02

5.718 ± 0.029

6.813 ± 0.031

4.956 ± 0.026

6.618 ± 0.03

1.038 ± 0.015

3.25 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski