Eubacterium sp. CAG:161

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5KZW7|R5KZW7_9FIRM Deoxyguanosinetriphosphate triphosphohydrolase-like protein OS=Eubacterium sp. CAG:161 OX=1262881 GN=BN508_01145 PE=4 SV=1
MM1 pKa = 7.26YY2 pKa = 10.86ALTDD6 pKa = 4.15YY7 pKa = 10.87IDD9 pKa = 4.58CEE11 pKa = 4.23ALADD15 pKa = 3.82TCEE18 pKa = 4.06EE19 pKa = 3.88NLAEE23 pKa = 5.17VEE25 pKa = 5.24DD26 pKa = 3.73IMNVKK31 pKa = 10.47KK32 pKa = 10.62NDD34 pKa = 3.76DD35 pKa = 3.76GLVVAEE41 pKa = 4.22NQAVV45 pKa = 3.09

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5KW07|R5KW07_9FIRM Glft2_N domain-containing protein OS=Eubacterium sp. CAG:161 OX=1262881 GN=BN508_01945 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.72VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2196

0

2196

721333

29

2449

328.5

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.384 ± 0.05

1.358 ± 0.019

6.029 ± 0.045

7.061 ± 0.06

4.014 ± 0.039

6.843 ± 0.045

1.423 ± 0.021

8.415 ± 0.065

8.78 ± 0.064

7.73 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.0 ± 0.031

5.796 ± 0.047

2.794 ± 0.025

2.523 ± 0.024

3.496 ± 0.038

6.02 ± 0.044

5.618 ± 0.055

7.204 ± 0.046

0.844 ± 0.017

4.662 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski