Planctomyces sp. SH-PL14

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces; unclassified Planctomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A142X417|A0A142X417_9PLAN Uncharacterized protein OS=Planctomyces sp. SH-PL14 OX=1632864 GN=VT03_27880 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84YY3 pKa = 8.64FVAMAAVVTAVQLAAGVASAQDD25 pKa = 3.6LASPDD30 pKa = 3.58EE31 pKa = 4.16RR32 pKa = 11.84VEE34 pKa = 3.97MYY36 pKa = 10.51EE37 pKa = 4.1PEE39 pKa = 5.15DD40 pKa = 3.82YY41 pKa = 11.23ASLRR45 pKa = 11.84ARR47 pKa = 11.84IEE49 pKa = 3.8QLEE52 pKa = 3.99ANQRR56 pKa = 11.84YY57 pKa = 8.37SAPAVASLDD66 pKa = 3.87PQGGVYY72 pKa = 9.69VQYY75 pKa = 11.26DD76 pKa = 3.62NVIVKK81 pKa = 9.77PYY83 pKa = 10.2LSEE86 pKa = 4.15NSAFPAQIADD96 pKa = 3.8GNVVNYY102 pKa = 9.74GFNLDD107 pKa = 3.59MEE109 pKa = 4.55YY110 pKa = 10.71SPRR113 pKa = 11.84IEE115 pKa = 4.26VGYY118 pKa = 8.01MAPTSNLGFRR128 pKa = 11.84ARR130 pKa = 11.84YY131 pKa = 6.65WHH133 pKa = 6.72FGANAATGPANDD145 pKa = 4.34ANGLIADD152 pKa = 4.32DD153 pKa = 5.54AIAIAAGATIQDD165 pKa = 3.43VDD167 pKa = 4.08TITTVVYY174 pKa = 10.31DD175 pKa = 4.18LNMDD179 pKa = 3.93VLDD182 pKa = 4.65LEE184 pKa = 4.42ATRR187 pKa = 11.84AVGSSGTFAAGIRR200 pKa = 11.84TAWMGQKK207 pKa = 10.33YY208 pKa = 9.96GLDD211 pKa = 3.47SDD213 pKa = 4.17EE214 pKa = 4.81GQLFTDD220 pKa = 4.72LDD222 pKa = 3.87VAAIGPTLAYY232 pKa = 9.0EE233 pKa = 4.45WKK235 pKa = 10.46SHH237 pKa = 6.56DD238 pKa = 3.35FWFDD242 pKa = 3.12GLRR245 pKa = 11.84GLATVRR251 pKa = 11.84GSLLYY256 pKa = 10.49GGKK259 pKa = 10.08DD260 pKa = 3.28INSVGPDD267 pKa = 2.95NTYY270 pKa = 10.26TYY272 pKa = 10.89NEE274 pKa = 3.79QSLISNADD282 pKa = 3.31LQLGLEE288 pKa = 4.27YY289 pKa = 10.29RR290 pKa = 11.84RR291 pKa = 11.84SGGFFLRR298 pKa = 11.84AALEE302 pKa = 3.92AQYY305 pKa = 9.63WANTGNATNHH315 pKa = 5.54NVGPGDD321 pKa = 3.86DD322 pKa = 3.91VGDD325 pKa = 4.07SEE327 pKa = 5.08FVGSILDD334 pKa = 4.11DD335 pKa = 4.15DD336 pKa = 5.07LGFLGMVFSAGYY348 pKa = 9.32SWW350 pKa = 3.81

Molecular weight:
37.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A142X5Y7|A0A142X5Y7_9PLAN Circadian clock protein KaiB OS=Planctomyces sp. SH-PL14 OX=1632864 GN=kaiB PE=4 SV=1
MM1 pKa = 7.66SKK3 pKa = 10.55RR4 pKa = 11.84SGGPARR10 pKa = 11.84RR11 pKa = 11.84PATIKK16 pKa = 10.79PNLQKK21 pKa = 11.03KK22 pKa = 8.34MRR24 pKa = 11.84SQLRR28 pKa = 11.84PLKK31 pKa = 9.58TVSSKK36 pKa = 11.08SKK38 pKa = 9.91SGKK41 pKa = 9.78SGTRR45 pKa = 3.18

Molecular weight:
4.92 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6497

0

6497

2309488

29

4696

355.5

38.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.74 ± 0.033

1.084 ± 0.012

5.602 ± 0.023

6.256 ± 0.025

3.606 ± 0.015

8.226 ± 0.032

2.109 ± 0.016

4.445 ± 0.022

3.442 ± 0.027

10.082 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.013

2.608 ± 0.019

6.05 ± 0.032

3.584 ± 0.019

7.555 ± 0.037

5.831 ± 0.022

5.541 ± 0.031

7.381 ± 0.025

1.609 ± 0.015

2.225 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski