Streptomyces sp. CB01580

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5H5G9|A0A1Q5H5G9_9ACTN Uncharacterized protein OS=Streptomyces sp. CB01580 OX=1703933 GN=AMK22_11090 PE=4 SV=1
MM1 pKa = 7.65NDD3 pKa = 3.41AEE5 pKa = 4.62LASLLAGCARR15 pKa = 11.84CPYY18 pKa = 9.43PGAWQDD24 pKa = 3.48SPFTEE29 pKa = 4.07RR30 pKa = 11.84TVDD33 pKa = 3.32GARR36 pKa = 11.84YY37 pKa = 9.69ALVAVDD43 pKa = 4.85PGLSALGVRR52 pKa = 11.84RR53 pKa = 11.84DD54 pKa = 4.38DD55 pKa = 3.83GSLWGLPEE63 pKa = 5.84DD64 pKa = 3.91GVPYY68 pKa = 10.36LVNSGVEE75 pKa = 3.75AFVAFNRR82 pKa = 11.84AYY84 pKa = 10.19EE85 pKa = 3.98EE86 pKa = 4.1AAAEE90 pKa = 4.01AAVYY94 pKa = 8.33EE95 pKa = 4.62GPGDD99 pKa = 4.03GPDD102 pKa = 3.15EE103 pKa = 4.62AEE105 pKa = 4.17AVDD108 pKa = 4.37LAEE111 pKa = 4.43EE112 pKa = 4.17AADD115 pKa = 3.94ALTEE119 pKa = 3.97ALVEE123 pKa = 4.09RR124 pKa = 11.84FGALDD129 pKa = 3.54AEE131 pKa = 4.58AVADD135 pKa = 5.14EE136 pKa = 4.1NSFWCVAAEE145 pKa = 3.76EE146 pKa = 4.66LGYY149 pKa = 11.37GMSVV153 pKa = 2.82

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5G2F3|A0A1Q5G2F3_9ACTN MFS transporter OS=Streptomyces sp. CB01580 OX=1703933 GN=AMK22_30465 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6429

2

6431

2125756

32

7771

330.5

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.503 ± 0.054

0.82 ± 0.008

6.093 ± 0.026

5.714 ± 0.034

2.718 ± 0.018

9.59 ± 0.033

2.342 ± 0.015

3.169 ± 0.022

2.114 ± 0.031

10.183 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.014

1.752 ± 0.018

6.161 ± 0.032

2.592 ± 0.017

8.284 ± 0.036

5.05 ± 0.02

6.136 ± 0.024

8.516 ± 0.034

1.464 ± 0.012

2.043 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski