Rhizobium mesoamericanum STM3625

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; Rhizobium mesoamericanum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0PV03|K0PV03_9RHIZ Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rhizobium mesoamericanum STM3625 OX=1211777 GN=sucB PE=3 SV=1
MM1 pKa = 6.91FTLAHH6 pKa = 6.81GGTITLPQGQTSVTVDD22 pKa = 2.87LHH24 pKa = 6.46FSADD28 pKa = 3.63TYY30 pKa = 11.22SGSISNSITSVTGQNEE46 pKa = 4.44YY47 pKa = 11.06EE48 pKa = 4.27SLLTTGSTSTTVNTPPTVVDD68 pKa = 3.67SEE70 pKa = 4.54NWLSSDD76 pKa = 3.31PAQYY80 pKa = 10.68TSGASSYY87 pKa = 10.26PNGYY91 pKa = 8.2PLHH94 pKa = 7.81IEE96 pKa = 4.24APTDD100 pKa = 3.48SDD102 pKa = 3.96HH103 pKa = 6.9NNLVVKK109 pKa = 10.61VSGTVPTGVFYY120 pKa = 11.15NGGSGYY126 pKa = 10.46VALTAGVTLYY136 pKa = 11.13DD137 pKa = 3.81LAHH140 pKa = 7.31GINLLDD146 pKa = 3.67SVVYY150 pKa = 10.4RR151 pKa = 11.84PTSSQTDD158 pKa = 3.61AQNLTLKK165 pKa = 10.62LDD167 pKa = 3.84VYY169 pKa = 11.15DD170 pKa = 3.88GTVHH174 pKa = 5.14VTQNVGIHH182 pKa = 5.47EE183 pKa = 4.65VPPTSLPSDD192 pKa = 3.74VQQIGSGGSPLTSGNDD208 pKa = 3.24QTQTLTLSQGTVNGILADD226 pKa = 3.76PHH228 pKa = 6.37GSTVVVYY235 pKa = 10.5TDD237 pKa = 3.81FQKK240 pKa = 11.21SPFATPIPADD250 pKa = 3.74EE251 pKa = 4.21QNPGAFGDD259 pKa = 4.63GSAGSARR266 pKa = 11.84EE267 pKa = 3.67QEE269 pKa = 4.45VQVEE273 pKa = 3.95IRR275 pKa = 11.84IGANRR280 pKa = 11.84FAVVEE285 pKa = 4.27DD286 pKa = 4.18DD287 pKa = 3.88RR288 pKa = 11.84SAGTFEE294 pKa = 4.37QSWFYY299 pKa = 11.63DD300 pKa = 3.65SASGLMKK307 pKa = 10.58AAVDD311 pKa = 3.6YY312 pKa = 11.35DD313 pKa = 4.1HH314 pKa = 7.63IYY316 pKa = 11.06LLDD319 pKa = 3.88AAGNATSTTLASYY332 pKa = 10.66LIGNPPTAGNTWTLVYY348 pKa = 10.29TDD350 pKa = 3.93NNGGNFQARR359 pKa = 11.84TVTFDD364 pKa = 4.1FFTHH368 pKa = 6.19NPGDD372 pKa = 3.55PGIPVTGNTTLADD385 pKa = 4.26TIYY388 pKa = 8.92GTSGEE393 pKa = 4.34DD394 pKa = 3.26VLTGSGGNDD403 pKa = 3.14MLYY406 pKa = 10.72GRR408 pKa = 11.84QDD410 pKa = 3.03HH411 pKa = 7.6DD412 pKa = 3.84ILNGGIGNDD421 pKa = 3.69MLTGGADD428 pKa = 3.6ADD430 pKa = 4.29TLTGGSGADD439 pKa = 3.2TFVIGSGEE447 pKa = 4.23SLGTTGGNGDD457 pKa = 3.9NGTISGNDD465 pKa = 3.39IITDD469 pKa = 4.44FDD471 pKa = 3.66TTADD475 pKa = 3.56ILNLAGTPFAAGNTSGWVDD494 pKa = 3.5GTNSSLTISSSQISKK509 pKa = 10.71HH510 pKa = 6.15SITNGIITFEE520 pKa = 4.03NASNNVVTLSSASQVAAVVQYY541 pKa = 9.76LHH543 pKa = 6.41QNDD546 pKa = 3.87MGNAGATVAFTATIGGTAHH565 pKa = 6.2TFIYY569 pKa = 9.44EE570 pKa = 4.25QVGDD574 pKa = 4.11TPDD577 pKa = 3.09ASKK580 pKa = 11.1DD581 pKa = 3.41ILVDD585 pKa = 3.94LQNVNLISGGTSLATLIGNSHH606 pKa = 7.33IDD608 pKa = 4.19PIVLDD613 pKa = 4.05LDD615 pKa = 4.16HH616 pKa = 7.07NGVALTSLDD625 pKa = 3.24QGVQFDD631 pKa = 4.25INADD635 pKa = 3.44GHH637 pKa = 6.51KK638 pKa = 10.7DD639 pKa = 3.44QIAWTTGSDD648 pKa = 3.69GILAFDD654 pKa = 3.48VDD656 pKa = 4.57GNGKK660 pKa = 9.03IDD662 pKa = 3.59NGSEE666 pKa = 3.55IFSPHH671 pKa = 5.93FAGGSYY677 pKa = 10.96VDD679 pKa = 4.32GLAALSALDD688 pKa = 4.14SNHH691 pKa = 7.05DD692 pKa = 3.82GKK694 pKa = 10.68IDD696 pKa = 3.64AADD699 pKa = 3.5EE700 pKa = 4.48AFSKK704 pKa = 9.35LTVWQDD710 pKa = 3.31LNHH713 pKa = 6.66NGITDD718 pKa = 3.61SGEE721 pKa = 4.0LSSLADD727 pKa = 3.63HH728 pKa = 6.91SISSISLEE736 pKa = 3.9ATASSTEE743 pKa = 3.85INGQSILADD752 pKa = 3.42GDD754 pKa = 3.87YY755 pKa = 10.65TLTDD759 pKa = 3.32GSTAHH764 pKa = 5.71FVEE767 pKa = 4.88VAFDD771 pKa = 3.59TTLGGSEE778 pKa = 4.12NSSNAYY784 pKa = 10.36SLIGSDD790 pKa = 4.51GDD792 pKa = 4.93DD793 pKa = 3.37ILSGSGGMFTISGGAGADD811 pKa = 3.66TFVLDD816 pKa = 4.84ADD818 pKa = 4.06ALNDD822 pKa = 3.48VKK824 pKa = 10.93LADD827 pKa = 4.12VITDD831 pKa = 4.01FKK833 pKa = 11.34ASEE836 pKa = 4.42GDD838 pKa = 3.46TLDD841 pKa = 3.29VSKK844 pKa = 11.07LLDD847 pKa = 3.59SLLGHH852 pKa = 5.88QASEE856 pKa = 4.47AEE858 pKa = 4.11ALASVKK864 pKa = 8.25TTVSGADD871 pKa = 3.11TVVSVNANGGWHH883 pKa = 6.85DD884 pKa = 3.83VAVLQNTTEE893 pKa = 3.9AVKK896 pKa = 10.53ILFDD900 pKa = 4.8DD901 pKa = 3.81KK902 pKa = 11.36HH903 pKa = 6.29DD904 pKa = 3.83TTTAPHH910 pKa = 5.91VGG912 pKa = 3.39

Molecular weight: 93.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0PYX2|K0PYX2_9RHIZ D-alanine--D-alanine ligase OS=Rhizobium mesoamericanum STM3625 OX=1211777 GN=ddlB PE=3 SV=1
MM1 pKa = 7.7DD2 pKa = 4.3TPAFAASVVALLILPGPTNAVLAMASHH29 pKa = 7.05GLRR32 pKa = 11.84LSRR35 pKa = 11.84MGVLLVAVILTYY47 pKa = 10.72LAVIVPVSLFGGPFLQQHH65 pKa = 6.29PAVSQGVKK73 pKa = 9.72LASATWMFYY82 pKa = 10.79LALKK86 pKa = 10.05LWGRR90 pKa = 11.84PSRR93 pKa = 11.84QMQPVAIGVRR103 pKa = 11.84QVVATTLLNPKK114 pKa = 10.11AIIIALAMLPSRR126 pKa = 11.84GILAPDD132 pKa = 4.02LFVSFVVAAGGASCMWLSIGRR153 pKa = 11.84LVLGEE158 pKa = 4.21GDD160 pKa = 4.05QMPLAARR167 pKa = 11.84RR168 pKa = 11.84CGSAVLLGFCVLLALSALL186 pKa = 4.0

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6358

0

6358

1835771

21

2839

288.7

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.614 ± 0.042

0.876 ± 0.011

5.593 ± 0.024

5.703 ± 0.029

3.956 ± 0.023

8.112 ± 0.027

2.105 ± 0.015

5.748 ± 0.025

3.826 ± 0.024

9.947 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.015

2.934 ± 0.016

4.837 ± 0.02

3.164 ± 0.017

6.745 ± 0.032

5.978 ± 0.024

5.328 ± 0.022

7.336 ± 0.021

1.307 ± 0.015

2.357 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski