Colwellia sp. MT41

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Colwellia; unclassified Colwellia

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3491 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2JHN5|A0A0S2JHN5_9GAMM Uncharacterized protein OS=Colwellia sp. MT41 OX=58049 GN=CMT41_12295 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.55VIMDD6 pKa = 5.33KK7 pKa = 10.54ISKK10 pKa = 10.35CFGQYY15 pKa = 8.94RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 10.82ALFATMILVLSLSGCGGSSYY38 pKa = 11.46GDD40 pKa = 3.45DD41 pKa = 3.86KK42 pKa = 11.53VTVDD46 pKa = 3.79NVAPIITLIGDD57 pKa = 3.43AEE59 pKa = 4.28VRR61 pKa = 11.84VLVNGSYY68 pKa = 10.58TDD70 pKa = 3.89AGASANDD77 pKa = 3.58NVDD80 pKa = 3.05GKK82 pKa = 10.82ISVAITGSVDD92 pKa = 2.71TMTAGSYY99 pKa = 10.38ILTYY103 pKa = 9.89TATDD107 pKa = 3.55YY108 pKa = 11.54AGNVSTLTRR117 pKa = 11.84TVTVVPPTLTGTAAAGAAIVGTVTIKK143 pKa = 10.99DD144 pKa = 3.63SLGEE148 pKa = 4.08TRR150 pKa = 11.84SEE152 pKa = 4.34LIEE155 pKa = 5.34ADD157 pKa = 3.04GTYY160 pKa = 10.7SVDD163 pKa = 3.56VTGLTAPFRR172 pKa = 11.84LRR174 pKa = 11.84AEE176 pKa = 4.17GTVGGKK182 pKa = 8.41SYY184 pKa = 10.41RR185 pKa = 11.84IHH187 pKa = 7.39SYY189 pKa = 11.23AEE191 pKa = 3.76EE192 pKa = 4.05ATLDD196 pKa = 3.43GTINITPFTDD206 pKa = 5.38LIIANAAHH214 pKa = 6.34QIAAAYY220 pKa = 9.08FDD222 pKa = 4.3EE223 pKa = 4.58VSPTFLDD230 pKa = 3.68PVEE233 pKa = 4.47IAAQEE238 pKa = 4.18DD239 pKa = 3.85ALQAKK244 pKa = 8.72LQAVFDD250 pKa = 4.37ALGLDD255 pKa = 3.77SAINLLTSAFSADD268 pKa = 3.69HH269 pKa = 6.58SGLDD273 pKa = 3.34AALDD277 pKa = 4.25IIQIEE282 pKa = 4.34TDD284 pKa = 4.3PITNIATIVNVLDD297 pKa = 4.18GSSIEE302 pKa = 5.41DD303 pKa = 5.46DD304 pKa = 3.58ITDD307 pKa = 3.44SDD309 pKa = 4.42DD310 pKa = 3.73NEE312 pKa = 4.36SVIIVDD318 pKa = 3.99SAALTIVVTDD328 pKa = 3.91TQAIAALFASFAAEE342 pKa = 4.11FTDD345 pKa = 4.44GLPSLASLDD354 pKa = 3.86GFFADD359 pKa = 4.88SFLHH363 pKa = 6.47EE364 pKa = 5.14DD365 pKa = 3.3QAKK368 pKa = 9.95SQFLTDD374 pKa = 3.21ITTDD378 pKa = 3.31PSLIGLVFSSIAVRR392 pKa = 11.84DD393 pKa = 4.08LDD395 pKa = 4.55SMAGTATVDD404 pKa = 3.65FNVYY408 pKa = 9.96INGIVDD414 pKa = 3.69VEE416 pKa = 4.44VEE418 pKa = 3.9TWLLQKK424 pKa = 10.73DD425 pKa = 4.32SSQAWQLLGNQRR437 pKa = 11.84FYY439 pKa = 11.26EE440 pKa = 4.58ADD442 pKa = 3.54LLTFHH447 pKa = 7.18CNDD450 pKa = 3.62FDD452 pKa = 5.88GSDD455 pKa = 4.1DD456 pKa = 3.7MAGGCGLNVSFYY468 pKa = 11.56DD469 pKa = 4.3NDD471 pKa = 3.35FSNNGTNDD479 pKa = 3.08APIASGTMTIIDD491 pKa = 3.97GTDD494 pKa = 3.05GEE496 pKa = 4.84TVKK499 pKa = 11.02DD500 pKa = 3.41IVYY503 pKa = 10.23LGNPDD508 pKa = 3.83YY509 pKa = 10.89IAAGEE514 pKa = 4.08LQVYY518 pKa = 8.88NQTTQMYY525 pKa = 8.07QWDD528 pKa = 3.98YY529 pKa = 11.75AGFGMSDD536 pKa = 3.77GEE538 pKa = 4.08VDD540 pKa = 3.46PNIFVVGDD548 pKa = 3.53IIRR551 pKa = 11.84YY552 pKa = 9.0KK553 pKa = 10.83LYY555 pKa = 10.47TEE557 pKa = 4.12QLDD560 pKa = 3.93LTVPGQPMVSSGNEE574 pKa = 3.23VAMFDD579 pKa = 4.54KK580 pKa = 10.74ILMYY584 pKa = 10.75LPEE587 pKa = 4.15TTGRR591 pKa = 11.84YY592 pKa = 7.56PALTTDD598 pKa = 4.94GLTALNNFTLDD609 pKa = 3.17EE610 pKa = 4.93DD611 pKa = 4.48LTVSWTLQPGTVIDD625 pKa = 4.7SIWVEE630 pKa = 3.66ISDD633 pKa = 4.09NQGNYY638 pKa = 10.14YY639 pKa = 10.52DD640 pKa = 4.96VNDD643 pKa = 4.11KK644 pKa = 11.11SIAPDD649 pKa = 3.52VTSTTVDD656 pKa = 2.97SSVFSQDD663 pKa = 4.02LLNDD667 pKa = 3.81LDD669 pKa = 4.47FDD671 pKa = 4.12QTNMTLLVRR680 pKa = 11.84IYY682 pKa = 10.56SIVPTTGQTHH692 pKa = 4.76STDD695 pKa = 3.22YY696 pKa = 10.94RR697 pKa = 11.84RR698 pKa = 11.84TFAGTMPGDD707 pKa = 3.57GTQPGDD713 pKa = 3.23GTATVMCNTEE723 pKa = 4.53SPWDD727 pKa = 3.94DD728 pKa = 5.02ANDD731 pKa = 3.78KK732 pKa = 9.04PTVFYY737 pKa = 10.71SINDD741 pKa = 3.51FEE743 pKa = 4.62TAVADD748 pKa = 4.2CASQASLLNFTKK760 pKa = 10.84ANLAGLTWYY769 pKa = 10.89VEE771 pKa = 4.2DD772 pKa = 4.0EE773 pKa = 4.59RR774 pKa = 11.84IVFDD778 pKa = 3.79SSADD782 pKa = 3.49TFVITTYY789 pKa = 11.41GDD791 pKa = 3.62DD792 pKa = 4.0GEE794 pKa = 5.61LGGGDD799 pKa = 4.66DD800 pKa = 3.57EE801 pKa = 4.79TFYY804 pKa = 10.8GTVADD809 pKa = 4.01YY810 pKa = 7.92ATNVIEE816 pKa = 4.86FSFSFTPGGDD826 pKa = 2.63IVGRR830 pKa = 11.84DD831 pKa = 4.15LIRR834 pKa = 11.84VKK836 pKa = 10.89AEE838 pKa = 3.95SSFQGKK844 pKa = 10.08VIFTAVLLWEE854 pKa = 4.86FYY856 pKa = 11.02DD857 pKa = 3.57WAEE860 pKa = 4.17SDD862 pKa = 4.14GDD864 pKa = 4.33NIDD867 pKa = 3.9KK868 pKa = 10.79AHH870 pKa = 6.4QGGEE874 pKa = 4.06LRR876 pKa = 11.84TAGYY880 pKa = 10.56AVDD883 pKa = 5.9ADD885 pKa = 4.29FDD887 pKa = 3.91WAAWQATPP895 pKa = 4.9

Molecular weight: 96.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2JKL8|A0A0S2JKL8_9GAMM EF-P post-translational modification enzyme B OS=Colwellia sp. MT41 OX=58049 GN=CMT41_03615 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.17RR12 pKa = 11.84KK13 pKa = 8.81RR14 pKa = 11.84NHH16 pKa = 5.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25SGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3491

0

3491

1168266

44

3376

334.7

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.661 ± 0.043

1.027 ± 0.016

5.521 ± 0.04

5.635 ± 0.035

4.28 ± 0.028

6.256 ± 0.04

2.23 ± 0.02

7.195 ± 0.032

6.153 ± 0.04

10.435 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.021

4.887 ± 0.032

3.467 ± 0.022

4.796 ± 0.043

3.858 ± 0.032

6.967 ± 0.035

5.534 ± 0.032

6.451 ± 0.031

1.101 ± 0.013

3.185 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski