Faecalibacterium phage FP_Lagaffe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lagaffevirus; Faecalibacterium virus Lagaffe

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9V2S9|A0A2K9V2S9_9CAUD Integrase OS=Faecalibacterium phage FP_Lagaffe OX=2070183 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84RR3 pKa = 11.84VTGYY7 pKa = 10.78KK8 pKa = 10.28DD9 pKa = 3.17YY10 pKa = 7.87TTRR13 pKa = 11.84EE14 pKa = 3.73GDD16 pKa = 3.51TFDD19 pKa = 4.66ALALEE24 pKa = 4.76MYY26 pKa = 9.96GEE28 pKa = 4.54EE29 pKa = 4.18MLAHH33 pKa = 6.79YY34 pKa = 9.87IAEE37 pKa = 4.33FNPDD41 pKa = 3.4YY42 pKa = 11.38ADD44 pKa = 3.77VLIFDD49 pKa = 4.71ANVALRR55 pKa = 11.84LPIVEE60 pKa = 4.17GAEE63 pKa = 4.3TPEE66 pKa = 4.1TLPPWRR72 pKa = 11.84RR73 pKa = 11.84DD74 pKa = 3.47SEE76 pKa = 5.22DD77 pKa = 3.8EE78 pKa = 4.62DD79 pKa = 5.62DD80 pKa = 4.29SAA82 pKa = 6.13

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9V316|A0A2K9V316_9CAUD Neck protein OS=Faecalibacterium phage FP_Lagaffe OX=2070183 PE=4 SV=1
MM1 pKa = 7.59GGLFYY6 pKa = 10.36TGQSRR11 pKa = 11.84RR12 pKa = 11.84TSGSFTVTCSGAEE25 pKa = 4.23DD26 pKa = 3.16IVKK29 pKa = 10.42RR30 pKa = 11.84LNKK33 pKa = 10.15LEE35 pKa = 4.64NGGQTAIKK43 pKa = 9.01KK44 pKa = 6.96TVNDD48 pKa = 3.88FMSRR52 pKa = 11.84APAWVSKK59 pKa = 10.2GIRR62 pKa = 11.84EE63 pKa = 4.26HH64 pKa = 7.37YY65 pKa = 10.67GVDD68 pKa = 2.97TAAINEE74 pKa = 4.34AKK76 pKa = 9.56QQPRR80 pKa = 11.84RR81 pKa = 11.84GQSSIQVAGVQVDD94 pKa = 3.96SASLVYY100 pKa = 10.16KK101 pKa = 10.67GRR103 pKa = 11.84TLTPTHH109 pKa = 6.41FKK111 pKa = 9.89MSPKK115 pKa = 9.95SRR117 pKa = 11.84PSAQQKK123 pKa = 10.06KK124 pKa = 9.23PIRR127 pKa = 11.84IPGQLIAGAGDD138 pKa = 3.48VAMVRR143 pKa = 11.84PPRR146 pKa = 11.84PYY148 pKa = 9.64QVKK151 pKa = 8.41ATIIKK156 pKa = 9.78GSRR159 pKa = 11.84VSLGSNVFLTGGNGGSMLPFQKK181 pKa = 9.38TGRR184 pKa = 11.84GRR186 pKa = 11.84SPIEE190 pKa = 3.56AVRR193 pKa = 11.84TLSVPQMIDD202 pKa = 2.52GKK204 pKa = 10.64ARR206 pKa = 11.84QTIEE210 pKa = 3.85TTISEE215 pKa = 4.36KK216 pKa = 10.59LGEE219 pKa = 4.42RR220 pKa = 11.84FNHH223 pKa = 6.83YY224 pKa = 9.68IDD226 pKa = 4.02QVMKK230 pKa = 10.88

Molecular weight: 24.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

15112

41

1263

232.5

25.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.098 ± 0.395

1.198 ± 0.129

5.995 ± 0.206

7.471 ± 0.282

2.925 ± 0.171

7.478 ± 0.229

1.529 ± 0.125

5.519 ± 0.205

6.478 ± 0.274

7.458 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.157

4.235 ± 0.143

4.136 ± 0.335

3.739 ± 0.222

5.155 ± 0.426

5.989 ± 0.528

6.875 ± 0.321

6.022 ± 0.216

1.648 ± 0.139

3.6 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski