Paenibacillus sp. IHBB 10380

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3V8A6|A0A0D3V8A6_9BACL GCN5 family acetyltransferase OS=Paenibacillus sp. IHBB 10380 OX=1566358 GN=UB51_08685 PE=4 SV=1
MM1 pKa = 7.23GVSVYY6 pKa = 6.97YY7 pKa = 10.39TCMRR11 pKa = 11.84NYY13 pKa = 10.8NLTNSEE19 pKa = 4.13EE20 pKa = 4.11QEE22 pKa = 3.57ITAIIDD28 pKa = 3.26KK29 pKa = 10.3YY30 pKa = 10.94NAGFEE35 pKa = 4.17MKK37 pKa = 10.46DD38 pKa = 2.96IGEE41 pKa = 4.43TFCVYY46 pKa = 10.44DD47 pKa = 3.89YY48 pKa = 11.48DD49 pKa = 4.02QDD51 pKa = 3.7EE52 pKa = 4.5PTVIFAGSTKK62 pKa = 10.63LPFSDD67 pKa = 5.16DD68 pKa = 4.21FEE70 pKa = 4.85DD71 pKa = 3.63TLHH74 pKa = 7.38ALFYY78 pKa = 10.51WLTCLTDD85 pKa = 3.12IRR87 pKa = 11.84RR88 pKa = 11.84SISNGDD94 pKa = 3.19WHH96 pKa = 6.73VHH98 pKa = 6.35LDD100 pKa = 3.66DD101 pKa = 5.15TDD103 pKa = 5.44AIWDD107 pKa = 4.33EE108 pKa = 4.19EE109 pKa = 4.78TGWQMPEE116 pKa = 3.92DD117 pKa = 3.74

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3VED3|A0A0D3VED3_9BACL Bcr/CflA family efflux transporter OS=Paenibacillus sp. IHBB 10380 OX=1566358 GN=UB51_20815 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.64RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.48GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4588

0

4588

1412272

30

10779

307.8

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.884 ± 0.039

0.787 ± 0.011

5.185 ± 0.032

6.907 ± 0.055

4.193 ± 0.031

6.929 ± 0.035

2.178 ± 0.018

7.694 ± 0.038

5.834 ± 0.031

10.064 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.0 ± 0.018

4.285 ± 0.027

3.644 ± 0.023

3.984 ± 0.027

4.498 ± 0.031

6.648 ± 0.027

5.467 ± 0.03

7.092 ± 0.03

1.157 ± 0.013

3.57 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski