Bdellovibrio bacteriovorus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150WR42|A0A150WR42_BDEBC Uncharacterized protein OS=Bdellovibrio bacteriovorus OX=959 GN=AZI86_08035 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 10.25NIAFIILMALTTTIAQGSGPQSSFWDD29 pKa = 3.65IIEE32 pKa = 4.44EE33 pKa = 4.16FNNCEE38 pKa = 4.32GPTCEE43 pKa = 4.84QIYY46 pKa = 9.55EE47 pKa = 4.22EE48 pKa = 4.88KK49 pKa = 10.62EE50 pKa = 4.06LVSEE54 pKa = 4.47GTLPAALEE62 pKa = 4.01KK63 pKa = 11.05ALMEE67 pKa = 4.2VAEE70 pKa = 4.35DD71 pKa = 3.82QVRR74 pKa = 11.84VWGDD78 pKa = 3.41TILEE82 pKa = 4.2GGYY85 pKa = 7.76ITRR88 pKa = 11.84GEE90 pKa = 4.32TEE92 pKa = 4.13LNEE95 pKa = 3.92VVAIYY100 pKa = 10.51KK101 pKa = 9.9NKK103 pKa = 10.35KK104 pKa = 8.7LIAYY108 pKa = 8.71RR109 pKa = 11.84IIYY112 pKa = 8.78SQAAINTDD120 pKa = 3.11SCDD123 pKa = 3.26YY124 pKa = 10.87DD125 pKa = 3.94YY126 pKa = 11.73EE127 pKa = 4.52NEE129 pKa = 4.14EE130 pKa = 4.16TLEE133 pKa = 4.37DD134 pKa = 3.94CTEE137 pKa = 3.94GRR139 pKa = 11.84ITEE142 pKa = 4.11TSIVSPDD149 pKa = 3.3FKK151 pKa = 11.01EE152 pKa = 3.74YY153 pKa = 10.75DD154 pKa = 3.31IDD156 pKa = 4.87GDD158 pKa = 4.15GYY160 pKa = 11.75ANFEE164 pKa = 4.42DD165 pKa = 4.04

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150WFH9|A0A150WFH9_BDEBC Uncharacterized protein OS=Bdellovibrio bacteriovorus OX=959 GN=AZI86_17805 PE=4 SV=1
MM1 pKa = 7.29VVIGAVLVIFRR12 pKa = 11.84VIPEE16 pKa = 4.12RR17 pKa = 11.84QTAATVAGVLFVLLPVILMVLEE39 pKa = 3.91YY40 pKa = 10.57RR41 pKa = 11.84RR42 pKa = 11.84AQLQEE47 pKa = 4.37MIWFVAVLQFWTVFALPILGIRR69 pKa = 11.84LLNWGVPFDD78 pKa = 3.56QLSFVGIPGPVLHH91 pKa = 6.09QFSSKK96 pKa = 10.43SYY98 pKa = 8.07MVMMIVTAWCWIKK111 pKa = 10.41LARR114 pKa = 11.84RR115 pKa = 11.84AQTT118 pKa = 3.14

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3712

0

3712

1191860

33

2224

321.1

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.295 ± 0.04

0.899 ± 0.014

5.114 ± 0.026

6.345 ± 0.048

4.665 ± 0.037

7.006 ± 0.046

1.909 ± 0.019

5.984 ± 0.033

6.813 ± 0.044

9.802 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.02

4.204 ± 0.028

4.093 ± 0.025

3.935 ± 0.025

4.693 ± 0.028

7.183 ± 0.044

5.421 ± 0.048

6.814 ± 0.032

1.211 ± 0.016

3.021 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski