Microbacterium esteraromaticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4JY53|A0A1R4JY53_9MICO Uncharacterized protein OS=Microbacterium esteraromaticum OX=57043 GN=FM104_09385 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AVKK5 pKa = 9.33RR6 pKa = 11.84TGLAVLASAALGLGALVATPAYY28 pKa = 9.8AATVDD33 pKa = 4.06DD34 pKa = 5.13VIDD37 pKa = 4.81GIIYY41 pKa = 9.89EE42 pKa = 4.68ADD44 pKa = 3.77DD45 pKa = 4.36ADD47 pKa = 4.03VAAGATVTGFDD58 pKa = 3.62GTLTTVNIPDD68 pKa = 3.62TVAIEE73 pKa = 4.02GTEE76 pKa = 3.96YY77 pKa = 11.21DD78 pKa = 3.8VVAIGNSAFVTYY90 pKa = 10.35RR91 pKa = 11.84SGGVITQGDD100 pKa = 3.55ITEE103 pKa = 4.28VVIGDD108 pKa = 3.61NVRR111 pKa = 11.84TIGLQAFMSNRR122 pKa = 11.84IDD124 pKa = 3.74SLTLGSSVTSIKK136 pKa = 10.66GSAFMGNSLTHH147 pKa = 5.25ITIPSAVTSIGGAAFAEE164 pKa = 4.15NDD166 pKa = 3.76DD167 pKa = 4.03LVSVVFTGPAPTVRR181 pKa = 11.84EE182 pKa = 3.7ADD184 pKa = 3.18AGYY187 pKa = 10.76SSASFSTRR195 pKa = 11.84DD196 pKa = 3.27TEE198 pKa = 4.28FVLRR202 pKa = 11.84FPEE205 pKa = 4.5QYY207 pKa = 10.62SADD210 pKa = 3.8NVAGGYY216 pKa = 6.55TTPLWFGYY224 pKa = 5.19TTEE227 pKa = 5.52AIGDD231 pKa = 4.09DD232 pKa = 4.18EE233 pKa = 5.76PDD235 pKa = 3.28ASLSITKK242 pKa = 8.87TVDD245 pKa = 2.68ASFVRR250 pKa = 11.84VYY252 pKa = 11.04DD253 pKa = 3.25WDD255 pKa = 3.9FEE257 pKa = 4.47KK258 pKa = 10.39TGTVGAITVDD268 pKa = 3.41GDD270 pKa = 3.53EE271 pKa = 4.81ASADD275 pKa = 3.24VDD277 pKa = 4.17FTVTATPNGYY287 pKa = 9.43VDD289 pKa = 3.43SYY291 pKa = 11.39YY292 pKa = 11.17VLEE295 pKa = 4.26GTITVLNEE303 pKa = 3.34GTEE306 pKa = 4.11AQTVDD311 pKa = 3.75VVDD314 pKa = 4.37VPDD317 pKa = 3.7VAASASCSVYY327 pKa = 10.74DD328 pKa = 5.1SSFQPAEE335 pKa = 3.99GVEE338 pKa = 4.25IAASEE343 pKa = 4.23SAEE346 pKa = 4.01FEE348 pKa = 4.26YY349 pKa = 10.85SCYY352 pKa = 10.22FDD354 pKa = 3.79GTPEE358 pKa = 4.99DD359 pKa = 4.02GTNTATVTWGDD370 pKa = 3.9DD371 pKa = 3.41QSASATADD379 pKa = 3.31VVFTQRR385 pKa = 11.84DD386 pKa = 3.49TSFQTITIGDD396 pKa = 3.77DD397 pKa = 3.25HH398 pKa = 7.73ADD400 pKa = 3.27PDD402 pKa = 4.6GPFEE406 pKa = 4.12EE407 pKa = 5.75LGTAEE412 pKa = 4.55WNSDD416 pKa = 3.36HH417 pKa = 7.1LPIEE421 pKa = 4.14FTYY424 pKa = 10.86TLTADD429 pKa = 3.49GLTVGEE435 pKa = 4.86CTTLTNTAAIYY446 pKa = 10.05EE447 pKa = 4.34MEE449 pKa = 4.61RR450 pKa = 11.84ATQASVEE457 pKa = 4.24ICPEE461 pKa = 3.92PAVVIPDD468 pKa = 4.33PEE470 pKa = 4.23PTPDD474 pKa = 4.42PEE476 pKa = 4.25PTPDD480 pKa = 4.55PEE482 pKa = 4.47PSPAPVVDD490 pKa = 4.56PEE492 pKa = 4.22PAGLATTGTDD502 pKa = 3.64FDD504 pKa = 4.71GGPLAIAGILLLVGVALLSIGRR526 pKa = 11.84RR527 pKa = 11.84YY528 pKa = 9.93RR529 pKa = 11.84PNGLLRR535 pKa = 11.84PP536 pKa = 4.11

Molecular weight: 55.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4KAK4|A0A1R4KAK4_9MICO Chromosome (Plasmid) partitioning protein ParA OS=Microbacterium esteraromaticum OX=57043 GN=FM104_11295 PE=4 SV=1
MM1 pKa = 6.49MTTMRR6 pKa = 11.84LFMVMRR12 pKa = 11.84VLARR16 pKa = 11.84TRR18 pKa = 11.84RR19 pKa = 11.84LTSGAMQPLPRR30 pKa = 11.84TRR32 pKa = 11.84ASAVSNSPP40 pKa = 3.11

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3202

0

3202

1007737

37

2035

314.7

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.275 ± 0.064

0.51 ± 0.011

6.375 ± 0.037

5.771 ± 0.043

3.073 ± 0.028

8.558 ± 0.035

2.099 ± 0.02

4.964 ± 0.031

2.184 ± 0.033

9.932 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.014 ± 0.018

2.026 ± 0.024

5.169 ± 0.032

3.037 ± 0.023

7.251 ± 0.054

5.767 ± 0.028

6.094 ± 0.033

8.484 ± 0.044

1.496 ± 0.019

1.922 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski