Vibrio phage K05K4_VK05K4_1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6UGC1|A0A1W6UGC1_9VIRU RstB protein OS=Vibrio phage K05K4_VK05K4_1 OX=1912326 GN=rstB _3 PE=4 SV=1
MM1 pKa = 6.8NTCFLDD7 pKa = 3.66TGISTHH13 pKa = 5.61AHH15 pKa = 5.7FEE17 pKa = 3.92KK18 pKa = 10.3TPYY21 pKa = 10.84SNARR25 pKa = 11.84YY26 pKa = 8.73PYY28 pKa = 9.25QTVCDD33 pKa = 3.76NGLGLGYY40 pKa = 10.63YY41 pKa = 7.75EE42 pKa = 4.43VRR44 pKa = 11.84CPEE47 pKa = 3.81NSEE50 pKa = 4.27FDD52 pKa = 3.99PSTLRR57 pKa = 11.84CKK59 pKa = 10.33SVCEE63 pKa = 4.04YY64 pKa = 11.13GKK66 pKa = 10.26NPDD69 pKa = 3.94GTCMDD74 pKa = 3.88ACQFKK79 pKa = 10.92KK80 pKa = 10.76SIDD83 pKa = 4.02EE84 pKa = 4.15IKK86 pKa = 10.48SLQWLAYY93 pKa = 9.88VYY95 pKa = 10.64GEE97 pKa = 4.11QVTGSCYY104 pKa = 10.68GDD106 pKa = 3.56YY107 pKa = 11.09GATRR111 pKa = 11.84CEE113 pKa = 4.25LEE115 pKa = 4.18RR116 pKa = 11.84TPSDD120 pKa = 3.21STLCTGVDD128 pKa = 3.27SGQWTQTPSVMVTFNSQATQCEE150 pKa = 4.55GGTLFWGKK158 pKa = 10.11DD159 pKa = 3.62GPDD162 pKa = 3.31TPIIPDD168 pKa = 4.37DD169 pKa = 5.14PIHH172 pKa = 7.61DD173 pKa = 4.88PDD175 pKa = 5.88DD176 pKa = 3.89PTGDD180 pKa = 3.64IEE182 pKa = 5.54DD183 pKa = 4.5PSILPDD189 pKa = 3.61GSTNTVNPPDD199 pKa = 4.02TDD201 pKa = 3.65SEE203 pKa = 4.63PDD205 pKa = 3.48VEE207 pKa = 5.55EE208 pKa = 5.22PDD210 pKa = 3.63TDD212 pKa = 3.49EE213 pKa = 4.45STDD216 pKa = 3.64TAVLKK221 pKa = 10.91AITGMNKK228 pKa = 10.0DD229 pKa = 3.38VNKK232 pKa = 10.54ALNDD236 pKa = 3.47MNIDD240 pKa = 3.4INQANADD247 pKa = 3.75VQNQIIALNASMVTNTQAIQKK268 pKa = 7.84QQINDD273 pKa = 3.14NKK275 pKa = 10.37IYY277 pKa = 10.49EE278 pKa = 4.16NTKK281 pKa = 10.95ALIQQANADD290 pKa = 3.49ITTAMNKK297 pKa = 7.52NTNAVNGVGDD307 pKa = 4.45DD308 pKa = 3.72VEE310 pKa = 5.55KK311 pKa = 10.74IAGAMDD317 pKa = 5.45GIAEE321 pKa = 4.26DD322 pKa = 3.65VSGISDD328 pKa = 3.9TLDD331 pKa = 4.1GIANTDD337 pKa = 3.08TSGAGTGGTCIEE349 pKa = 4.43SQSCTGFYY357 pKa = 10.69EE358 pKa = 4.04SGYY361 pKa = 9.68PDD363 pKa = 3.62GLGGLVSGQLDD374 pKa = 3.73DD375 pKa = 5.58LKK377 pKa = 11.35HH378 pKa = 4.84NTIDD382 pKa = 3.42NFVNSFGDD390 pKa = 3.73LDD392 pKa = 4.02LSSAKK397 pKa = 10.1RR398 pKa = 11.84PSFVLPVPFFGDD410 pKa = 3.76FSFEE414 pKa = 4.0EE415 pKa = 4.36QISFDD420 pKa = 3.97LLFGLFVRR428 pKa = 11.84CSSS431 pKa = 3.36

Molecular weight: 46.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6UFW1|A0A1W6UFW1_9VIRU Uncharacterized protein OS=Vibrio phage K05K4_VK05K4_1 OX=1912326 GN=K05K4_52540 PE=4 SV=1
MM1 pKa = 7.41AKK3 pKa = 9.95SVFVLGMDD11 pKa = 3.58ITWNSARR18 pKa = 11.84GDD20 pKa = 3.84SAQLNVSRR28 pKa = 11.84PLRR31 pKa = 11.84EE32 pKa = 3.83INSEE36 pKa = 3.91KK37 pKa = 10.33FKK39 pKa = 11.11RR40 pKa = 11.84RR41 pKa = 11.84ALSVNPVMM49 pKa = 5.81

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

5065

49

491

180.9

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.029 ± 0.405

2.113 ± 0.252

6.693 ± 0.55

5.429 ± 0.228

4.423 ± 0.187

6.752 ± 0.392

1.856 ± 0.203

5.884 ± 0.305

6.199 ± 0.41

8.687 ± 0.549

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.276

4.877 ± 0.404

4.284 ± 0.238

4.166 ± 0.299

4.837 ± 0.477

6.614 ± 0.305

6.338 ± 0.47

6.12 ± 0.357

1.461 ± 0.159

3.672 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski