Bacteroides phage crAss001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DVW5|A0A385DVW5_9CAUD Thiamine biosynthesis protein OS=Bacteroides phage crAss001 OX=2301731 GN=crAss001_76 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 6.01DD3 pKa = 3.66EE4 pKa = 4.45VLEE7 pKa = 4.24SAISQMTIDD16 pKa = 4.96AEE18 pKa = 4.59TAGLGHH24 pKa = 7.71PDD26 pKa = 4.18GIIIQTAIVGGSTYY40 pKa = 10.9APEE43 pKa = 4.33EE44 pKa = 3.79NLMYY48 pKa = 10.87SPASEE53 pKa = 4.32LNSAIALLQSGGWEE67 pKa = 4.09IIHH70 pKa = 6.42EE71 pKa = 4.26EE72 pKa = 3.99QHH74 pKa = 6.19VSGAYY79 pKa = 9.84LVTIGAVSMEE89 pKa = 4.27TPMLPVTLVVTLDD102 pKa = 3.83GSNLLHH108 pKa = 7.34DD109 pKa = 4.58AQNTEE114 pKa = 4.18DD115 pKa = 4.51APVEE119 pKa = 3.78IDD121 pKa = 3.61EE122 pKa = 4.29QGEE125 pKa = 4.21ALLEE129 pKa = 4.07AALAAEE135 pKa = 4.4EE136 pKa = 4.59VVIPPNSGSLLVDD149 pKa = 3.64EE150 pKa = 4.73ATSRR154 pKa = 11.84FSGAIWYY161 pKa = 9.21SAIQSKK167 pKa = 9.42TITLAGVGGIGSYY180 pKa = 10.75VGFLLARR187 pKa = 11.84LKK189 pKa = 10.66PAGLYY194 pKa = 10.5LYY196 pKa = 10.57DD197 pKa = 4.57PDD199 pKa = 4.54IVEE202 pKa = 4.15QANMSGQLYY211 pKa = 10.27GSQDD215 pKa = 3.11LGQAKK220 pKa = 9.91VSSLHH225 pKa = 7.28RR226 pKa = 11.84MLQVYY231 pKa = 10.18ANYY234 pKa = 9.22YY235 pKa = 10.36NSVAYY240 pKa = 8.15QEE242 pKa = 4.97RR243 pKa = 11.84FTDD246 pKa = 3.62EE247 pKa = 4.96SEE249 pKa = 4.03ATDD252 pKa = 4.01IMICGFDD259 pKa = 3.29NMEE262 pKa = 3.92ARR264 pKa = 11.84KK265 pKa = 9.99LFFDD269 pKa = 3.19KK270 pKa = 10.54WFEE273 pKa = 4.09HH274 pKa = 6.35VGNKK278 pKa = 9.84PEE280 pKa = 4.28GDD282 pKa = 3.09RR283 pKa = 11.84SKK285 pKa = 11.31CLFIDD290 pKa = 3.61GRR292 pKa = 11.84LAAEE296 pKa = 4.47EE297 pKa = 4.12FQVFAIQGNDD307 pKa = 2.47EE308 pKa = 4.13RR309 pKa = 11.84AIVEE313 pKa = 4.34YY314 pKa = 10.54KK315 pKa = 10.75NRR317 pKa = 11.84WLFSDD322 pKa = 4.39AVADD326 pKa = 3.99EE327 pKa = 5.06TICSYY332 pKa = 11.16KK333 pKa = 9.36QTTFMANMIASVMVNLFVNFVANEE357 pKa = 4.04CNPIIDD363 pKa = 3.65RR364 pKa = 11.84DD365 pKa = 4.16VPFMTQYY372 pKa = 11.26SADD375 pKa = 3.07TMYY378 pKa = 10.94FKK380 pKa = 11.42VEE382 pKa = 3.88MM383 pKa = 4.8

Molecular weight: 42.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DTE1|A0A385DTE1_9CAUD Putative HNH endonuclease OS=Bacteroides phage crAss001 OX=2301731 GN=crAss001_8 PE=4 SV=1
MM1 pKa = 7.48SKK3 pKa = 10.79GRR5 pKa = 11.84VLTKK9 pKa = 10.5KK10 pKa = 10.18KK11 pKa = 8.98WGHH14 pKa = 5.44CARR17 pKa = 11.84YY18 pKa = 7.11WRR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 8.89KK23 pKa = 10.86YY24 pKa = 8.98RR25 pKa = 11.84TKK27 pKa = 10.59FPRR30 pKa = 11.84KK31 pKa = 9.19KK32 pKa = 10.31LNSFEE37 pKa = 4.2GTHH40 pKa = 6.42APIGYY45 pKa = 8.01PLKK48 pKa = 9.24YY49 pKa = 10.08LSWIMRR55 pKa = 3.81

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

31616

33

2412

304.0

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.902 ± 0.32

1.025 ± 0.148

6.041 ± 0.22

7.423 ± 0.305

4.213 ± 0.134

6.174 ± 0.27

1.215 ± 0.09

7.085 ± 0.242

7.695 ± 0.307

8.078 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.146

6.671 ± 0.291

3.318 ± 0.102

3.59 ± 0.195

4.438 ± 0.196

6.373 ± 0.199

5.807 ± 0.224

6.206 ± 0.18

1.253 ± 0.075

4.931 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski