Pepper huasteco yellow vein virus (PHYVV) (Pepper huasteco virus)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q06924|TRAP_PHUV Transcriptional activator protein OS=Pepper huasteco yellow vein virus OX=223303 GN=AC2 PE=3 SV=1
MM1 pKa = 7.89PLPKK5 pKa = 9.96RR6 pKa = 11.84FRR8 pKa = 11.84LNAKK12 pKa = 9.88NYY14 pKa = 9.14FLTYY18 pKa = 7.87PQCSISKK25 pKa = 9.36EE26 pKa = 3.74EE27 pKa = 5.21RR28 pKa = 11.84LAQLQNLSTPVNKK41 pKa = 10.15KK42 pKa = 9.85YY43 pKa = 10.85IKK45 pKa = 9.55ICKK48 pKa = 9.06EE49 pKa = 3.78SHH51 pKa = 6.99EE52 pKa = 4.97DD53 pKa = 3.77GQPHH57 pKa = 6.46LHH59 pKa = 6.28VLIQFEE65 pKa = 4.74GKK67 pKa = 8.79YY68 pKa = 9.48QCTNNRR74 pKa = 11.84FFDD77 pKa = 4.15LVSSTRR83 pKa = 11.84SAHH86 pKa = 5.12FHH88 pKa = 6.64PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.94VKK101 pKa = 10.48TYY103 pKa = 10.41IDD105 pKa = 3.73KK106 pKa = 11.39DD107 pKa = 3.34GDD109 pKa = 3.88TVEE112 pKa = 4.0WGEE115 pKa = 3.78FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 4.05TYY134 pKa = 11.89AKK136 pKa = 10.46ALNSASAEE144 pKa = 3.93EE145 pKa = 3.84ALQIIKK151 pKa = 10.28EE152 pKa = 4.13EE153 pKa = 4.13QPQHH157 pKa = 6.63FFLQFHH163 pKa = 6.85NIVSNANRR171 pKa = 11.84IFQTPPEE178 pKa = 4.11PWVPPFQQASFNNVPAIMTQWVSDD202 pKa = 4.16NVCDD206 pKa = 3.86AAARR210 pKa = 11.84PMRR213 pKa = 11.84PLSLVVEE220 pKa = 5.02GPSRR224 pKa = 11.84TGKK227 pKa = 7.64TLWARR232 pKa = 11.84SLGPHH237 pKa = 6.53NYY239 pKa = 9.18ICGHH243 pKa = 6.39MDD245 pKa = 4.49LSPKK249 pKa = 9.97IYY251 pKa = 10.74SNNAWYY257 pKa = 10.47NVIDD261 pKa = 6.02DD262 pKa = 4.54IPPHH266 pKa = 4.93YY267 pKa = 10.38VKK269 pKa = 10.57HH270 pKa = 6.04FKK272 pKa = 10.84EE273 pKa = 4.16FMGAQRR279 pKa = 11.84DD280 pKa = 3.71WQSNCKK286 pKa = 9.1YY287 pKa = 10.37GKK289 pKa = 9.39PIQIKK294 pKa = 10.42GGIPTIFLCNPGPQSSYY311 pKa = 11.03KK312 pKa = 10.59DD313 pKa = 3.56YY314 pKa = 11.34LSEE317 pKa = 4.1EE318 pKa = 4.53KK319 pKa = 10.51NRR321 pKa = 11.84SLNDD325 pKa = 3.45WVQKK329 pKa = 9.49NAIYY333 pKa = 9.09VTIEE337 pKa = 3.52EE338 pKa = 5.11AIFTTGSQTSPP349 pKa = 3.16

Molecular weight: 39.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q06923|REP_PHUV Replication-associated protein OS=Pepper huasteco yellow vein virus OX=223303 GN=AC1 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.8APWRR9 pKa = 11.84LTAGTAKK16 pKa = 10.39ISRR19 pKa = 11.84TGNNSRR25 pKa = 11.84ALIMGPSTSRR35 pKa = 11.84ASAWVNRR42 pKa = 11.84PMYY45 pKa = 9.63RR46 pKa = 11.84KK47 pKa = 8.62PRR49 pKa = 11.84IYY51 pKa = 10.76RR52 pKa = 11.84MYY54 pKa = 9.61RR55 pKa = 11.84TPDD58 pKa = 3.19VPKK61 pKa = 10.64GCEE64 pKa = 4.39GPCKK68 pKa = 10.23VQSFEE73 pKa = 3.83QRR75 pKa = 11.84HH76 pKa = 5.53DD77 pKa = 3.55VSHH80 pKa = 5.99VGKK83 pKa = 10.07VICISDD89 pKa = 3.51VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMYY154 pKa = 10.66DD155 pKa = 3.86NEE157 pKa = 4.34PSTATVKK164 pKa = 10.81NDD166 pKa = 2.84LRR168 pKa = 11.84DD169 pKa = 3.6RR170 pKa = 11.84YY171 pKa = 10.16QVMHH175 pKa = 7.0RR176 pKa = 11.84FYY178 pKa = 11.53AKK180 pKa = 9.12VTGGQYY186 pKa = 11.1ASNEE190 pKa = 3.84QALVRR195 pKa = 11.84RR196 pKa = 11.84FWKK199 pKa = 10.4VNNHH203 pKa = 4.48VVYY206 pKa = 10.67NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.77ATLKK240 pKa = 9.27IRR242 pKa = 11.84VYY244 pKa = 10.58FYY246 pKa = 11.3DD247 pKa = 4.39SIMNN251 pKa = 4.24

Molecular weight: 29.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1186

60

349

197.7

22.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.565 ± 0.462

1.939 ± 0.281

4.469 ± 0.391

3.794 ± 0.472

4.553 ± 0.467

5.143 ± 0.369

3.288 ± 0.404

5.565 ± 0.539

6.324 ± 0.393

7.083 ± 1.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.583

6.492 ± 0.471

5.312 ± 0.727

4.3 ± 0.87

6.83 ± 0.978

8.347 ± 1.299

6.071 ± 0.838

6.324 ± 0.978

1.518 ± 0.243

4.469 ± 0.589
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski