Pseudobutyrivibrio ruminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Pseudobutyrivibrio

Average proteome isoelectric point is 5.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7IIU3|A0A1H7IIU3_9FIRM Phosphatidate cytidylyltransferase OS=Pseudobutyrivibrio ruminis OX=46206 GN=SAMN02910377_01319 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.31KK3 pKa = 10.23KK4 pKa = 10.59LLALVLAATMVAGLAGCGNSSADD27 pKa = 3.55TASTGSASTASTSTDD42 pKa = 3.48LNVMIEE48 pKa = 4.26TAVEE52 pKa = 4.1SLDD55 pKa = 3.65PQQATDD61 pKa = 3.57GTSFEE66 pKa = 4.7VIADD70 pKa = 3.82YY71 pKa = 11.2TDD73 pKa = 4.31GLMQMDD79 pKa = 4.15SDD81 pKa = 4.32GNPINALAEE90 pKa = 4.38SYY92 pKa = 10.89DD93 pKa = 3.87LSDD96 pKa = 5.66DD97 pKa = 3.59GLTYY101 pKa = 10.06TFKK104 pKa = 10.71IRR106 pKa = 11.84DD107 pKa = 3.66DD108 pKa = 4.56ANWSNGEE115 pKa = 4.1PVTAQDD121 pKa = 6.31FVFAWQRR128 pKa = 11.84AVDD131 pKa = 3.83PEE133 pKa = 4.27VASEE137 pKa = 3.84YY138 pKa = 10.9SYY140 pKa = 10.83MLSDD144 pKa = 3.58IGQIVNAAEE153 pKa = 4.31IIAGEE158 pKa = 4.1KK159 pKa = 10.32DD160 pKa = 3.25KK161 pKa = 11.97SEE163 pKa = 4.61LGVTAVDD170 pKa = 4.05DD171 pKa = 3.81KK172 pKa = 11.34TLEE175 pKa = 4.07VQLNVPVSYY184 pKa = 10.12FLSLMYY190 pKa = 10.48FPTFYY195 pKa = 10.31PVNEE199 pKa = 4.19AFFNEE204 pKa = 4.57CGDD207 pKa = 3.88TFATSPEE214 pKa = 4.25TVLSNGAFVLDD225 pKa = 4.22SYY227 pKa = 11.36EE228 pKa = 3.99PAATAFHH235 pKa = 6.11LTKK238 pKa = 10.92NEE240 pKa = 4.06DD241 pKa = 3.72YY242 pKa = 11.04YY243 pKa = 11.62DD244 pKa = 3.98AASVSLSGLSYY255 pKa = 10.63QVIQDD260 pKa = 3.58SQTALMSYY268 pKa = 8.02QTGDD272 pKa = 3.47LDD274 pKa = 3.53ITLVNGDD281 pKa = 3.47QVDD284 pKa = 3.89QVEE287 pKa = 5.09DD288 pKa = 3.85DD289 pKa = 4.94PEE291 pKa = 4.23FQSIGAGYY299 pKa = 9.78LWYY302 pKa = 9.08VTPNIKK308 pKa = 9.8EE309 pKa = 4.16VPEE312 pKa = 3.93LANLNIRR319 pKa = 11.84LALTMAINRR328 pKa = 11.84EE329 pKa = 4.29SVTADD334 pKa = 3.27VLKK337 pKa = 10.96DD338 pKa = 3.61GSLPTYY344 pKa = 8.72TAIPRR349 pKa = 11.84DD350 pKa = 3.89FSADD354 pKa = 3.54FSADD358 pKa = 3.17QEE360 pKa = 4.37QFKK363 pKa = 10.66DD364 pKa = 3.6YY365 pKa = 11.18CRR367 pKa = 11.84DD368 pKa = 3.47DD369 pKa = 3.38AAAAAEE375 pKa = 4.13YY376 pKa = 8.58WQAGLDD382 pKa = 3.52EE383 pKa = 5.25LGITEE388 pKa = 4.41LTLEE392 pKa = 4.37LVADD396 pKa = 4.51EE397 pKa = 5.53DD398 pKa = 4.19DD399 pKa = 4.72APAKK403 pKa = 10.2VATVLKK409 pKa = 9.96EE410 pKa = 3.88EE411 pKa = 4.59WEE413 pKa = 4.37TTLPGLTVNITTEE426 pKa = 4.0PKK428 pKa = 9.2KK429 pKa = 10.85QRR431 pKa = 11.84VSDD434 pKa = 3.94LQEE437 pKa = 3.86GNYY440 pKa = 10.26EE441 pKa = 4.13LGLTRR446 pKa = 11.84WGPDD450 pKa = 2.85YY451 pKa = 10.75DD452 pKa = 5.83DD453 pKa = 4.26PMTYY457 pKa = 10.67LGMWYY462 pKa = 9.0TDD464 pKa = 3.13NPNNYY469 pKa = 9.04GFWSNAEE476 pKa = 3.55YY477 pKa = 10.71DD478 pKa = 4.43AILDD482 pKa = 3.75EE483 pKa = 4.86CTTGDD488 pKa = 3.72LCTEE492 pKa = 3.74IDD494 pKa = 3.37ARR496 pKa = 11.84RR497 pKa = 11.84DD498 pKa = 3.36RR499 pKa = 11.84LYY501 pKa = 10.9DD502 pKa = 3.41AEE504 pKa = 4.25QIVMEE509 pKa = 4.46EE510 pKa = 3.75AVIFPIYY517 pKa = 9.3TAANAQMVSSNVSGIDD533 pKa = 3.52FHH535 pKa = 7.18PVALNRR541 pKa = 11.84VYY543 pKa = 11.28KK544 pKa = 9.87NTTKK548 pKa = 10.93NN549 pKa = 3.07

Molecular weight: 60.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7JNL3|A0A1H7JNL3_9FIRM Chromosome partitioning protein OS=Pseudobutyrivibrio ruminis OX=46206 GN=SAMN02910377_01749 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.0VHH16 pKa = 5.93GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.72VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2521

0

2521

841775

41

3439

333.9

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.603 ± 0.052

1.389 ± 0.018

6.427 ± 0.046

7.343 ± 0.046

4.249 ± 0.036

6.878 ± 0.046

1.612 ± 0.021

7.941 ± 0.048

6.742 ± 0.039

8.436 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.024 ± 0.027

4.93 ± 0.04

3.029 ± 0.029

2.811 ± 0.023

3.56 ± 0.036

6.126 ± 0.056

5.586 ± 0.055

7.135 ± 0.039

0.825 ± 0.017

4.354 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski