Metschnikowia bicuspidata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Metschnikowia

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P9ZH10|A0A4P9ZH10_9ASCO Uncharacterized protein OS=Metschnikowia bicuspidata OX=27322 GN=METBISCDRAFT_25643 PE=4 SV=1
MM1 pKa = 7.67SEE3 pKa = 3.99GATPAEE9 pKa = 4.02QLLEE13 pKa = 4.01CARR16 pKa = 11.84RR17 pKa = 11.84NNTEE21 pKa = 3.74LFLEE25 pKa = 5.02ISAQLGDD32 pKa = 4.21GPALGDD38 pKa = 4.77LINSTTEE45 pKa = 4.12TVSGNGPLHH54 pKa = 5.78ITTLIGNWEE63 pKa = 4.51VLDD66 pKa = 3.8MMLDD70 pKa = 3.63VAGVEE75 pKa = 3.69IDD77 pKa = 3.36PTNRR81 pKa = 11.84EE82 pKa = 4.09LATPLHH88 pKa = 6.22LAVKK92 pKa = 9.56YY93 pKa = 10.41AADD96 pKa = 4.17EE97 pKa = 4.56PEE99 pKa = 4.14HH100 pKa = 7.44GYY102 pKa = 10.83FIVDD106 pKa = 3.58NLLDD110 pKa = 4.42AGSNSCAPDD119 pKa = 3.07IHH121 pKa = 6.42GLRR124 pKa = 11.84PANYY128 pKa = 8.6VCNNPEE134 pKa = 4.16LLEE137 pKa = 4.04LLKK140 pKa = 10.82SAEE143 pKa = 4.28YY144 pKa = 9.66AAGMAAVPAAADD156 pKa = 3.83DD157 pKa = 4.48EE158 pKa = 4.84EE159 pKa = 6.47DD160 pKa = 3.82GSASEE165 pKa = 4.81SEE167 pKa = 4.24

Molecular weight: 17.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P9ZDY4|A0A4P9ZDY4_9ASCO NADH-ubiquinone oxidoreductase B12 subunit OS=Metschnikowia bicuspidata OX=27322 GN=METBISCDRAFT_15937 PE=3 SV=1
MM1 pKa = 7.24QKK3 pKa = 10.07RR4 pKa = 11.84FKK6 pKa = 11.05SRR8 pKa = 11.84GNTYY12 pKa = 10.1QPSTLRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 8.87RR21 pKa = 11.84TFGFLARR28 pKa = 11.84LRR30 pKa = 11.84SRR32 pKa = 11.84NGRR35 pKa = 11.84KK36 pKa = 8.98ILARR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 10.24AKK44 pKa = 9.91GRR46 pKa = 11.84WYY48 pKa = 9.08LTHH51 pKa = 7.23

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4707

0

4707

2058784

49

3838

437.4

49.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.048

1.423 ± 0.012

5.805 ± 0.026

6.197 ± 0.034

4.427 ± 0.022

5.443 ± 0.028

2.437 ± 0.015

5.123 ± 0.029

5.955 ± 0.037

10.729 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.014

4.288 ± 0.023

4.649 ± 0.024

3.79 ± 0.022

5.37 ± 0.032

7.634 ± 0.033

5.32 ± 0.018

6.896 ± 0.028

1.075 ± 0.011

3.233 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski