Vibrio phage vB_VpS_CA8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7K9M5|A0A6B7K9M5_9CAUD Uncharacterized protein OS=Vibrio phage vB_VpS_CA8 OX=2594693 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 4.89INKK5 pKa = 9.55LCYY8 pKa = 9.86VKK10 pKa = 10.38PALEE14 pKa = 4.43EE15 pKa = 3.89LLQAYY20 pKa = 7.53NWKK23 pKa = 9.58RR24 pKa = 11.84CEE26 pKa = 3.91HH27 pKa = 6.04TGMLEE32 pKa = 3.82TLDD35 pKa = 3.94YY36 pKa = 11.54DD37 pKa = 4.68EE38 pKa = 5.2EE39 pKa = 4.45TLLDD43 pKa = 3.85DD44 pKa = 3.85VFNSIEE50 pKa = 4.26VCCTGAMRR58 pKa = 11.84HH59 pKa = 5.38RR60 pKa = 11.84VSDD63 pKa = 3.85MVTTWVLCDD72 pKa = 3.65SLLTLEE78 pKa = 4.67DD79 pKa = 3.38HH80 pKa = 7.12SGLLFIHH87 pKa = 5.86YY88 pKa = 6.85TTGEE92 pKa = 4.16EE93 pKa = 3.97VDD95 pKa = 4.11RR96 pKa = 11.84PAA98 pKa = 5.83

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C6XW97|A0A6C6XW97_9CAUD Uncharacterized protein OS=Vibrio phage vB_VpS_CA8 OX=2594693 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.05LTTTSAVRR11 pKa = 11.84ALRR14 pKa = 11.84KK15 pKa = 8.84AAKK18 pKa = 9.71SAGVQVGRR26 pKa = 11.84IAISDD31 pKa = 3.6RR32 pKa = 11.84TEE34 pKa = 3.44VGLIDD39 pKa = 4.13NTTKK43 pKa = 9.03MWSGRR48 pKa = 11.84VQLHH52 pKa = 4.58WCKK55 pKa = 10.82GSVEE59 pKa = 3.87LMQRR63 pKa = 11.84YY64 pKa = 9.03LEE66 pKa = 4.17NTNLVLNARR75 pKa = 11.84PCGDD79 pKa = 2.84GHH81 pKa = 6.72FSVSVNVAA89 pKa = 2.88

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

17565

41

842

209.1

23.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.397 ± 0.488

1.497 ± 0.157

5.739 ± 0.223

6.775 ± 0.293

3.695 ± 0.151

7.583 ± 0.233

2.226 ± 0.18

5.232 ± 0.176

6.149 ± 0.296

7.794 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.029 ± 0.139

4.406 ± 0.165

4.093 ± 0.217

4.27 ± 0.488

5.511 ± 0.212

5.232 ± 0.213

6.2 ± 0.288

7.145 ± 0.264

1.702 ± 0.137

3.325 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski