Erwinia phage PEp14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2DE88|H2DE88_9CAUD Uncharacterized protein OS=Erwinia phage PEp14 OX=1131315 GN=PEp14_00058 PE=4 SV=1
MM1 pKa = 7.72IEE3 pKa = 4.53TIAGWMLVIAICGPAPDD20 pKa = 4.54DD21 pKa = 4.71CRR23 pKa = 11.84DD24 pKa = 3.76EE25 pKa = 4.84PVDD28 pKa = 4.18DD29 pKa = 4.1YY30 pKa = 11.49FYY32 pKa = 10.41STKK35 pKa = 10.88AMCEE39 pKa = 4.0NLSGKK44 pKa = 8.66ATTDD48 pKa = 3.33PDD50 pKa = 4.1AVCVPVEE57 pKa = 3.43ITRR60 pKa = 11.84RR61 pKa = 11.84VEE63 pKa = 3.84APP65 pKa = 3.04

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2DE69|H2DE69_9CAUD Uncharacterized protein OS=Erwinia phage PEp14 OX=1131315 GN=PEp14_00039 PE=4 SV=1
MM1 pKa = 6.65MAAIKK6 pKa = 8.37THH8 pKa = 5.05TGIVVRR14 pKa = 11.84ASGRR18 pKa = 11.84KK19 pKa = 8.69RR20 pKa = 11.84VQLHH24 pKa = 4.91QSATAWIARR33 pKa = 11.84SNEE36 pKa = 3.56YY37 pKa = 10.6YY38 pKa = 10.86YY39 pKa = 10.78RR40 pKa = 11.84DD41 pKa = 2.98TGARR45 pKa = 11.84VGGYY49 pKa = 9.95GRR51 pKa = 11.84GHH53 pKa = 6.95IEE55 pKa = 4.18LDD57 pKa = 3.83SIRR60 pKa = 11.84PIAPAAAQGGAAA72 pKa = 4.27

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

19341

35

4914

302.2

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.918 ± 0.494

0.62 ± 0.173

6.23 ± 0.244

6.799 ± 0.346

3.164 ± 0.168

8.143 ± 0.297

1.799 ± 0.154

3.966 ± 0.148

4.808 ± 0.245

7.435 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.123

3.836 ± 0.172

5.113 ± 0.333

4.819 ± 0.311

6.68 ± 0.311

5.517 ± 0.206

5.843 ± 0.2

6.215 ± 0.293

1.742 ± 0.18

2.544 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski