Phytoplasma australiense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrXII (Stolbur group)

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1V9I2|B1V9I2_PHYAS Uncharacterized protein OS=Phytoplasma australiense OX=59748 GN=PA0269 PE=4 SV=1
MM1 pKa = 7.76NYY3 pKa = 9.91QKK5 pKa = 9.6NTTTYY10 pKa = 10.79CNIDD14 pKa = 3.17GKK16 pKa = 9.04TIYY19 pKa = 10.21AIHH22 pKa = 6.22EE23 pKa = 4.53HH24 pKa = 7.55DD25 pKa = 4.55PDD27 pKa = 3.09TWNFIKK33 pKa = 8.19TTWFNKK39 pKa = 9.11NGKK42 pKa = 7.48TIDD45 pKa = 4.04YY46 pKa = 8.47ITEE49 pKa = 3.98YY50 pKa = 10.88DD51 pKa = 4.03PEE53 pKa = 4.27TEE55 pKa = 4.12EE56 pKa = 4.99PIKK59 pKa = 9.57EE60 pKa = 4.19TYY62 pKa = 9.84YY63 pKa = 11.3NSDD66 pKa = 3.05GTIKK70 pKa = 11.12GEE72 pKa = 4.22TTFF75 pKa = 4.61

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1V9P5|B1V9P5_PHYAS Uncharacterized protein OS=Phytoplasma australiense OX=59748 GN=PA0332 PE=4 SV=1
MM1 pKa = 7.53SNPFKK6 pKa = 10.4TLNCMRR12 pKa = 11.84INKK15 pKa = 5.9TTEE18 pKa = 3.48RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 10.8LMTPQFRR28 pKa = 11.84RR29 pKa = 11.84NATWFFAILALLGMIFISYY48 pKa = 10.79RR49 pKa = 11.84LFNTNRR55 pKa = 11.84TQDD58 pKa = 3.63PQAGGSEE65 pKa = 4.29ALRR68 pKa = 11.84HH69 pKa = 4.3QTQTEE74 pKa = 4.3AEE76 pKa = 4.14RR77 pKa = 11.84KK78 pKa = 9.21AAEE81 pKa = 4.23TNQPQANTGSQEE93 pKa = 3.68

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

633

0

633

180110

36

2026

284.5

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.421 ± 0.083

0.892 ± 0.028

4.54 ± 0.061

6.334 ± 0.122

5.769 ± 0.113

4.367 ± 0.089

1.88 ± 0.045

9.26 ± 0.092

11.144 ± 0.123

10.726 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.036

7.193 ± 0.112

3.255 ± 0.062

5.299 ± 0.1

2.954 ± 0.066

5.607 ± 0.065

5.336 ± 0.076

4.829 ± 0.093

0.636 ± 0.028

3.764 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski