Rhodococcus sp. (strain AD45)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D8HWK3|A0A0D8HWK3_RHOSX Uncharacterized protein OS=Rhodococcus sp. (strain AD45) OX=103808 GN=SZ00_03018 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84QWLFAIVASAALAASGCSYY22 pKa = 11.16DD23 pKa = 3.82ATDD26 pKa = 4.57SGTDD30 pKa = 3.32ADD32 pKa = 4.76NVTVMPTSLSPPAAADD48 pKa = 3.43AVLLKK53 pKa = 9.9TADD56 pKa = 3.81SPLGTIAVDD65 pKa = 3.81GEE67 pKa = 4.3GMTVYY72 pKa = 10.09IYY74 pKa = 10.88DD75 pKa = 4.44PDD77 pKa = 4.06EE78 pKa = 3.95EE79 pKa = 5.2HH80 pKa = 6.92PTGDD84 pKa = 4.12SCDD87 pKa = 3.9EE88 pKa = 3.95SCLRR92 pKa = 11.84HH93 pKa = 5.4WPAVTSVTDD102 pKa = 3.48SPVTEE107 pKa = 5.53GINAALGTVPGPDD120 pKa = 3.08GSFQVTVNGKK130 pKa = 8.5PVYY133 pKa = 9.92RR134 pKa = 11.84YY135 pKa = 10.35LDD137 pKa = 3.87DD138 pKa = 4.53EE139 pKa = 4.91VPGDD143 pKa = 3.81MLGQAVGSLWFMIDD157 pKa = 3.06EE158 pKa = 4.76FGNPVFTTEE167 pKa = 3.66

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D8HZN9|A0A0D8HZN9_RHOSX Polysaccharide pyruvyl transferase CsaB OS=Rhodococcus sp. (strain AD45) OX=103808 GN=SZ00_00376 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6266

0

6266

2061266

29

9534

329.0

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.178 ± 0.036

0.755 ± 0.009

6.191 ± 0.026

5.562 ± 0.022

3.177 ± 0.017

8.762 ± 0.031

2.102 ± 0.015

4.812 ± 0.022

2.437 ± 0.023

9.864 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.013

2.423 ± 0.018

5.274 ± 0.024

2.86 ± 0.017

6.565 ± 0.028

6.354 ± 0.022

6.32 ± 0.026

8.775 ± 0.035

1.421 ± 0.012

2.081 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski