Mycobacterium phage Smeagol

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JTB2|A0A2P1JTB2_9CAUD Uncharacterized protein OS=Mycobacterium phage Smeagol OX=2108131 GN=68 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 9.35YY3 pKa = 7.78TLRR6 pKa = 11.84VFGIPVLSFEE16 pKa = 4.47SAGTGAEE23 pKa = 3.56EE24 pKa = 5.43GYY26 pKa = 10.65INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JT85|A0A2P1JT85_9CAUD Uncharacterized protein OS=Mycobacterium phage Smeagol OX=2108131 GN=37 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84EE3 pKa = 3.65LWGNDD8 pKa = 2.41ARR10 pKa = 11.84KK11 pKa = 8.79WLIRR15 pKa = 11.84KK16 pKa = 9.05SPHH19 pKa = 3.91TQEE22 pKa = 4.3WIVFPSVGSFYY33 pKa = 11.27GVITFHH39 pKa = 7.49PDD41 pKa = 3.0YY42 pKa = 10.33EE43 pKa = 4.34SARR46 pKa = 11.84AGFIRR51 pKa = 11.84QTRR54 pKa = 11.84RR55 pKa = 11.84PP56 pKa = 3.6

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16397

32

827

182.2

20.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.173 ± 0.328

0.561 ± 0.093

6.69 ± 0.266

6.641 ± 0.278

3.177 ± 0.165

8.404 ± 0.47

1.952 ± 0.165

4.909 ± 0.215

4.251 ± 0.275

8.642 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.025 ± 0.116

3.116 ± 0.145

5.489 ± 0.229

3.171 ± 0.177

6.318 ± 0.411

6.001 ± 0.3

6.458 ± 0.307

7.081 ± 0.207

2.067 ± 0.109

2.872 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski