Ostreococcus lucimarinus virus OlV4

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 294 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9E4Z6|G9E4Z6_9PHYC Uncharacterized protein OS=Ostreococcus lucimarinus virus OlV4 OX=754063 GN=OLOG_00057 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 9.76TGFGEE7 pKa = 4.06TSGAYY12 pKa = 8.32EE13 pKa = 4.63QSQQDD18 pKa = 3.73ALMGILLPVLEE29 pKa = 4.89RR30 pKa = 11.84SMILAAEE37 pKa = 4.1YY38 pKa = 10.97SKK40 pKa = 11.59ACGRR44 pKa = 11.84DD45 pKa = 3.4TVLSEE50 pKa = 4.07DD51 pKa = 3.1MEE53 pKa = 4.51YY54 pKa = 10.69AIKK57 pKa = 10.3YY58 pKa = 8.79CVMYY62 pKa = 9.98TVGQDD67 pKa = 2.82IGSLFPEE74 pKa = 4.9IYY76 pKa = 10.34NEE78 pKa = 3.99EE79 pKa = 4.26SSDD82 pKa = 3.67EE83 pKa = 3.98EE84 pKa = 5.24DD85 pKa = 3.56IEE87 pKa = 4.43EE88 pKa = 4.43VEE90 pKa = 4.52PDD92 pKa = 3.27EE93 pKa = 5.16CPPFVRR99 pKa = 11.84YY100 pKa = 10.12SGADD104 pKa = 3.12PTFRR108 pKa = 11.84QMNEE112 pKa = 3.4ACDD115 pKa = 4.5RR116 pKa = 11.84WDD118 pKa = 2.91TWIPQSPVEE127 pKa = 4.22EE128 pKa = 4.05MLKK131 pKa = 10.56NAINSNEE138 pKa = 3.98YY139 pKa = 10.09IGAGGLDD146 pKa = 3.47NFF148 pKa = 4.92

Molecular weight: 16.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9E5L9|G9E5L9_9PHYC Uncharacterized protein OS=Ostreococcus lucimarinus virus OlV4 OX=754063 GN=OLOG_00291 PE=4 SV=1
MM1 pKa = 7.15HH2 pKa = 6.75VKK4 pKa = 10.18IVRR7 pKa = 11.84SPDD10 pKa = 2.92RR11 pKa = 11.84KK12 pKa = 10.1KK13 pKa = 10.68KK14 pKa = 10.13FRR16 pKa = 11.84AILGDD21 pKa = 3.19GRR23 pKa = 11.84TVDD26 pKa = 5.31FGASGYY32 pKa = 10.89SDD34 pKa = 3.53YY35 pKa = 10.9TKK37 pKa = 10.58HH38 pKa = 6.49KK39 pKa = 9.02NPSRR43 pKa = 11.84MRR45 pKa = 11.84SYY47 pKa = 11.14VLRR50 pKa = 11.84HH51 pKa = 6.09GGQIPKK57 pKa = 10.13RR58 pKa = 11.84IVAEE62 pKa = 4.0RR63 pKa = 11.84QPAMIHH69 pKa = 6.5RR70 pKa = 11.84MMRR73 pKa = 11.84DD74 pKa = 2.55IDD76 pKa = 3.95KK77 pKa = 10.91SDD79 pKa = 3.54KK80 pKa = 10.84EE81 pKa = 4.12DD82 pKa = 3.19WKK84 pKa = 11.39LSGIGGAGFWSRR96 pKa = 11.84WYY98 pKa = 10.29LWSQPTIPEE107 pKa = 4.01VQRR110 pKa = 11.84FMAKK114 pKa = 9.77RR115 pKa = 11.84FGIKK119 pKa = 10.36FII121 pKa = 4.63

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

294

0

294

60727

44

1620

206.6

23.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.572 ± 0.168

1.764 ± 0.128

5.866 ± 0.139

6.761 ± 0.2

4.071 ± 0.118

5.922 ± 0.26

2.065 ± 0.094

6.44 ± 0.14

8.0 ± 0.283

8.156 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.099

6.216 ± 0.31

3.969 ± 0.128

3.456 ± 0.114

5.159 ± 0.193

6.101 ± 0.133

6.106 ± 0.234

6.468 ± 0.203

1.037 ± 0.058

3.95 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski