Pelosinus fermentans B4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Pelosinus; Pelosinus fermentans

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I9L7M1|I9L7M1_9FIRM ABC transporter transmembrane region OS=Pelosinus fermentans B4 OX=1149862 GN=FB4_0855 PE=4 SV=1
MM1 pKa = 7.36SFDD4 pKa = 3.88DD5 pKa = 3.47TSMTRR10 pKa = 11.84FSPTTPKK17 pKa = 10.36FPLCTSPANCCCIDD31 pKa = 3.5TTKK34 pKa = 9.59PTGATGITGATGATGATGATGATGATGAAGAASATGATGATGATGATGATGAAGAASATGATGATGATGATGATGAAGAASATGATGATGATGATGDD131 pKa = 4.07AGATGATGATGATGDD146 pKa = 4.07AGATGATGVTGATGTTGDD164 pKa = 3.95TGATGATGVAGATGTTGDD182 pKa = 3.95TGATGATGVAGATGATGVAGATGTTGDD209 pKa = 3.95TGATGATGVAGATGTTGDD227 pKa = 3.95TGATGATGVAGATGTTGDD245 pKa = 3.95TGATGATGVAGATGVTGDD263 pKa = 3.99TGATGATGATGTTGATGAVGATGATGDD290 pKa = 4.07AGATGATGATGAGAIIPFASGGPVTLTTIAGGLVGTTSLVGFGSSATGISLLGGVIDD347 pKa = 4.28LTGTALGPIIDD358 pKa = 4.71FSFSVPRR365 pKa = 11.84DD366 pKa = 3.49GVITSIAAYY375 pKa = 9.82FSNTLALALVGSTITITAEE394 pKa = 4.29LYY396 pKa = 10.64EE397 pKa = 4.59STTPDD402 pKa = 3.04NTFTAIPGAIVTLAPALTGVVALGAISNGIITGLNIPVTPEE443 pKa = 3.34TRR445 pKa = 11.84LLLVFSATAAGLTLINTVTGYY466 pKa = 11.39ASAGLNIVV474 pKa = 3.39

Molecular weight: 41.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I9L7X1|I9L7X1_9FIRM Multi antimicrobial extrusion protein MatE OS=Pelosinus fermentans B4 OX=1149862 GN=FB4_0857 PE=4 SV=1
MM1 pKa = 7.45VEE3 pKa = 4.04PRR5 pKa = 11.84LVSDD9 pKa = 4.42LNRR12 pKa = 11.84FGIGRR17 pKa = 11.84PKK19 pKa = 10.55SIVGSRR25 pKa = 11.84GSAVVGGQIVICLVRR40 pKa = 11.84LYY42 pKa = 10.77GSPTVVVWSKK52 pKa = 11.25GMYY55 pKa = 9.5SGRR58 pKa = 11.84AKK60 pKa = 9.82TAMGSEE66 pKa = 4.85MIRR69 pKa = 11.84VGRR72 pKa = 11.84CRR74 pKa = 11.84EE75 pKa = 3.66IATGSVGICGSRR87 pKa = 11.84AKK89 pKa = 10.84VSIGCVMLVGSRR101 pKa = 11.84KK102 pKa = 8.4IAYY105 pKa = 8.85RR106 pKa = 11.84YY107 pKa = 9.44VMIGIGCSVDD117 pKa = 3.01HH118 pKa = 6.63YY119 pKa = 11.81GLFLRR124 pKa = 11.84LADD127 pKa = 4.26LFRR130 pKa = 6.25

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4690

0

4690

1436960

30

3760

306.4

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.172 ± 0.04

1.153 ± 0.014

4.887 ± 0.027

6.454 ± 0.038

3.996 ± 0.027

7.326 ± 0.042

1.982 ± 0.015

8.229 ± 0.038

6.32 ± 0.035

9.753 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.88 ± 0.019

4.327 ± 0.034

3.636 ± 0.023

3.835 ± 0.027

4.186 ± 0.032

5.966 ± 0.025

5.407 ± 0.031

7.22 ± 0.031

0.955 ± 0.014

3.316 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski