Roseomonas cervicalis ATCC 49957

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas; Roseomonas cervicalis

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5RIW8|D5RIW8_9PROT Carbonic anhydrase OS=Roseomonas cervicalis ATCC 49957 OX=525371 GN=cah PE=3 SV=1
MM1 pKa = 7.5AVLIGTPGNDD11 pKa = 2.81RR12 pKa = 11.84LIAPDD17 pKa = 3.41ARR19 pKa = 11.84EE20 pKa = 3.99NDD22 pKa = 4.41SIAGDD27 pKa = 3.71AGDD30 pKa = 4.86DD31 pKa = 4.06FIEE34 pKa = 4.84ARR36 pKa = 11.84GGDD39 pKa = 3.9DD40 pKa = 5.18RR41 pKa = 11.84ITPGPGNDD49 pKa = 2.98RR50 pKa = 11.84VEE52 pKa = 4.71GGDD55 pKa = 3.52GRR57 pKa = 11.84DD58 pKa = 3.44TVIVSGDD65 pKa = 3.0ISQTEE70 pKa = 4.43VYY72 pKa = 9.82RR73 pKa = 11.84YY74 pKa = 10.25NNEE77 pKa = 3.76GVLRR81 pKa = 11.84GPDD84 pKa = 3.66GVDD87 pKa = 2.79TLLDD91 pKa = 3.53VEE93 pKa = 5.15AVQFTGVGGTLEE105 pKa = 4.01MSDD108 pKa = 3.72ANSFLSYY115 pKa = 10.79SYY117 pKa = 10.14IASYY121 pKa = 11.53GDD123 pKa = 3.26LTEE126 pKa = 5.76AYY128 pKa = 9.78GADD131 pKa = 3.52AGAGWRR137 pKa = 11.84HH138 pKa = 5.18FRR140 pKa = 11.84DD141 pKa = 4.68FGAVEE146 pKa = 3.9GRR148 pKa = 11.84EE149 pKa = 3.67ITFNGNAYY157 pKa = 10.04LAANTDD163 pKa = 3.61VLSALGANADD173 pKa = 3.67EE174 pKa = 4.8SGARR178 pKa = 11.84HH179 pKa = 4.89YY180 pKa = 11.18LEE182 pKa = 4.01YY183 pKa = 11.18GRR185 pKa = 11.84FEE187 pKa = 4.03GRR189 pKa = 11.84TTEE192 pKa = 4.45FAALSYY198 pKa = 8.42TASYY202 pKa = 10.93GEE204 pKa = 5.1LIDD207 pKa = 5.37SFGTDD212 pKa = 3.86TIAATAHH219 pKa = 6.08FVQEE223 pKa = 4.32GFNEE227 pKa = 3.93GRR229 pKa = 11.84GISFNGLEE237 pKa = 4.3YY238 pKa = 10.34VASYY242 pKa = 11.23GDD244 pKa = 4.91LIDD247 pKa = 5.11AYY249 pKa = 10.55GDD251 pKa = 3.4AEE253 pKa = 4.3RR254 pKa = 11.84PFDD257 pKa = 4.99LGEE260 pKa = 5.04DD261 pKa = 4.04GAGHH265 pKa = 6.82YY266 pKa = 9.18IQYY269 pKa = 10.6GRR271 pKa = 11.84GEE273 pKa = 4.15GRR275 pKa = 11.84EE276 pKa = 4.04TTFDD280 pKa = 3.22GLQYY284 pKa = 8.82MASYY288 pKa = 10.8GDD290 pKa = 3.65VIEE293 pKa = 4.77AFRR296 pKa = 11.84DD297 pKa = 3.67STDD300 pKa = 2.66AGAYY304 pKa = 7.13DD305 pKa = 4.25TIGALHH311 pKa = 6.9YY312 pKa = 10.59IRR314 pKa = 11.84DD315 pKa = 3.82GFGEE319 pKa = 4.07EE320 pKa = 3.57RR321 pKa = 11.84VADD324 pKa = 4.17RR325 pKa = 11.84FNEE328 pKa = 3.92QSYY331 pKa = 10.52AAANGDD337 pKa = 3.73LAEE340 pKa = 4.8AGITSADD347 pKa = 3.28ALALHH352 pKa = 7.57WIQYY356 pKa = 9.77GYY358 pKa = 11.13EE359 pKa = 3.9KK360 pKa = 10.73GRR362 pKa = 11.84AGAYY366 pKa = 10.32DD367 pKa = 3.67PVIAA371 pKa = 5.43

Molecular weight: 39.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5RJG8|D5RJG8_9PROT Uncharacterized protein (Fragment) OS=Roseomonas cervicalis ATCC 49957 OX=525371 GN=HMPREF0731_1228 PE=4 SV=1
MM1 pKa = 7.36GRR3 pKa = 11.84GGTLGRR9 pKa = 11.84AAAVPWRR16 pKa = 11.84SGARR20 pKa = 11.84LRR22 pKa = 11.84AAAIRR27 pKa = 11.84WGGKK31 pKa = 9.41AGSGAIPWRR40 pKa = 11.84GRR42 pKa = 11.84RR43 pKa = 11.84PRR45 pKa = 11.84AGRR48 pKa = 11.84QAWHH52 pKa = 6.99PAASS56 pKa = 3.41

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4774

0

4774

1327154

39

2039

278.0

29.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.034 ± 0.058

0.815 ± 0.009

4.747 ± 0.028

5.728 ± 0.038

3.198 ± 0.021

9.444 ± 0.038

1.981 ± 0.018

3.933 ± 0.026

1.952 ± 0.029

11.628 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.017

1.929 ± 0.019

6.302 ± 0.029

3.339 ± 0.025

8.53 ± 0.042

4.467 ± 0.024

4.602 ± 0.025

6.859 ± 0.029

1.447 ± 0.016

1.758 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski