Escherichia phage Pollock

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Humphriesvirinae; Pollockvirus; Escherichia virus Pollock

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0YR07|A0A0A0YR07_9CAUD Uncharacterized protein OS=Escherichia phage Pollock OX=1540097 GN=CPT_Pollock67 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.28LVLHH6 pKa = 6.19IKK8 pKa = 10.01EE9 pKa = 4.43RR10 pKa = 11.84GRR12 pKa = 11.84KK13 pKa = 7.84PPLMYY18 pKa = 9.96MGLFSRR24 pKa = 11.84KK25 pKa = 9.47KK26 pKa = 8.44IISVSSVTVNLAGDD40 pKa = 3.6DD41 pKa = 4.32AEE43 pKa = 5.28NFLQKK48 pKa = 9.79TITTAVMNGSGIGSTLNQAYY68 pKa = 10.0LNGMGTQIRR77 pKa = 11.84QAYY80 pKa = 9.49RR81 pKa = 11.84YY82 pKa = 9.47GRR84 pKa = 11.84DD85 pKa = 3.62YY86 pKa = 11.62YY87 pKa = 11.65SLGLPDD93 pKa = 4.7GSIFYY98 pKa = 9.2VVPNYY103 pKa = 10.6DD104 pKa = 3.7DD105 pKa = 4.97LMTVLSSLNEE115 pKa = 3.98GKK117 pKa = 10.05EE118 pKa = 4.04VSIVMSDD125 pKa = 3.65YY126 pKa = 11.35NVADD130 pKa = 4.08LSYY133 pKa = 9.87WIEE136 pKa = 3.98RR137 pKa = 11.84YY138 pKa = 7.93LTNTYY143 pKa = 10.81DD144 pKa = 2.91WDD146 pKa = 3.73ADD148 pKa = 3.77YY149 pKa = 11.56GGMGNPPAGVASTATIDD166 pKa = 3.62WSIDD170 pKa = 3.2NNGVVTITMGTGSEE184 pKa = 3.81IDD186 pKa = 3.48FTEE189 pKa = 3.94QVSFSDD195 pKa = 3.15INYY198 pKa = 9.05GSEE201 pKa = 4.51YY202 pKa = 8.9YY203 pKa = 9.85QVIYY207 pKa = 10.29RR208 pKa = 11.84VRR210 pKa = 11.84TPGTPEE216 pKa = 3.55VTTEE220 pKa = 3.52TRR222 pKa = 11.84AYY224 pKa = 10.37EE225 pKa = 4.27EE226 pKa = 4.52GDD228 pKa = 3.65EE229 pKa = 4.44EE230 pKa = 5.03GTTTDD235 pKa = 3.25EE236 pKa = 4.57TINNNFGLITSTVTTVQATINEE258 pKa = 4.64DD259 pKa = 3.25KK260 pKa = 11.02TEE262 pKa = 4.16TTITTTVVVSTLSRR276 pKa = 11.84KK277 pKa = 9.82KK278 pKa = 10.36YY279 pKa = 10.1FIYY282 pKa = 10.29EE283 pKa = 4.41AGSGTYY289 pKa = 9.96PILDD293 pKa = 4.44KK294 pKa = 11.03ILTDD298 pKa = 3.48VVRR301 pKa = 11.84DD302 pKa = 3.46SAYY305 pKa = 10.57YY306 pKa = 8.85PVVPLRR312 pKa = 11.84VNNTDD317 pKa = 3.07LTDD320 pKa = 4.35PNNVSAEE327 pKa = 3.94QFKK330 pKa = 10.2TSKK333 pKa = 9.71TLLNKK338 pKa = 10.35LNLRR342 pKa = 11.84FTDD345 pKa = 5.18LGDD348 pKa = 3.91TLNEE352 pKa = 4.01NPDD355 pKa = 3.4IKK357 pKa = 11.1DD358 pKa = 2.9IDD360 pKa = 3.61HH361 pKa = 7.06AFFVMGISLNSKK373 pKa = 8.71YY374 pKa = 10.22EE375 pKa = 3.74SSIDD379 pKa = 3.56YY380 pKa = 10.23LHH382 pKa = 7.09EE383 pKa = 4.24FFKK386 pKa = 11.4YY387 pKa = 10.05LAQVSPNDD395 pKa = 3.39KK396 pKa = 10.2EE397 pKa = 5.76NYY399 pKa = 8.2IKK401 pKa = 10.37WYY403 pKa = 8.04TEE405 pKa = 3.64NVNEE409 pKa = 4.82EE410 pKa = 4.21GTISNTDD417 pKa = 2.88SKK419 pKa = 11.3PPVNRR424 pKa = 11.84LSLRR428 pKa = 11.84QDD430 pKa = 3.45PYY432 pKa = 11.3DD433 pKa = 3.21ITIAYY438 pKa = 8.86QYY440 pKa = 11.63SDD442 pKa = 3.63LTVKK446 pKa = 10.61NGSIGSIGTVTRR458 pKa = 11.84SVGNKK463 pKa = 8.18ATINVVNILNASVEE477 pKa = 4.2MTADD481 pKa = 2.86VSTILFRR488 pKa = 11.84KK489 pKa = 9.41QISEE493 pKa = 4.09TQYY496 pKa = 11.26EE497 pKa = 4.19EE498 pKa = 5.07VEE500 pKa = 4.19ICGLEE505 pKa = 4.22HH506 pKa = 6.89INYY509 pKa = 9.0IYY511 pKa = 10.36KK512 pKa = 10.12GHH514 pKa = 6.16SVNITGEE521 pKa = 4.07ASLSDD526 pKa = 3.83PDD528 pKa = 4.22NEE530 pKa = 4.41GFLIPLCANIVDD542 pKa = 4.04AQQIVHH548 pKa = 6.29RR549 pKa = 11.84TQMTYY554 pKa = 10.7DD555 pKa = 3.66CLHH558 pKa = 6.5IVVNSYY564 pKa = 10.26QVTKK568 pKa = 10.83AKK570 pKa = 9.69WYY572 pKa = 8.81QSGIFKK578 pKa = 10.7VVTIAIAVVIAVYY591 pKa = 10.87SLGTLSAGITAAASAATAAGTSVAAAVAVYY621 pKa = 8.68ITTQVAIGLAISFGVSILAKK641 pKa = 9.77FVSPNIMFIATIAMLTYY658 pKa = 10.16GAVSAYY664 pKa = 9.87QSYY667 pKa = 8.19GTTGNKK673 pKa = 9.07GLPYY677 pKa = 9.45ATEE680 pKa = 4.01VMNLVPSVAKK690 pKa = 9.81GAQMRR695 pKa = 11.84LQQDD699 pKa = 3.52LQNIVKK705 pKa = 9.88EE706 pKa = 4.12MQKK709 pKa = 10.25NAEE712 pKa = 4.08NYY714 pKa = 7.8KK715 pKa = 10.47KK716 pKa = 10.41QMEE719 pKa = 4.64EE720 pKa = 4.14IEE722 pKa = 4.44DD723 pKa = 3.69AMDD726 pKa = 4.23ALGTINPNIDD736 pKa = 2.87IDD738 pKa = 4.05ALINSAFFNLFEE750 pKa = 4.42QPDD753 pKa = 3.67EE754 pKa = 4.4FFTRR758 pKa = 11.84TLSTNPGVVTLEE770 pKa = 4.28SIEE773 pKa = 4.61NYY775 pKa = 9.98VDD777 pKa = 3.54YY778 pKa = 11.19ALTLPTDD785 pKa = 4.35LNSMRR790 pKa = 11.84SS791 pKa = 3.34

Molecular weight: 87.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0YUA7|A0A0A0YUA7_9CAUD Uncharacterized protein OS=Escherichia phage Pollock OX=1540097 GN=CPT_Pollock79 PE=4 SV=1
MM1 pKa = 7.71AKK3 pKa = 8.95LTSKK7 pKa = 10.11QRR9 pKa = 11.84NKK11 pKa = 10.82LPDD14 pKa = 3.33SAFAGPDD21 pKa = 2.66RR22 pKa = 11.84SYY24 pKa = 10.94PVHH27 pKa = 7.29DD28 pKa = 4.44KK29 pKa = 11.39AHH31 pKa = 6.41AANAKK36 pKa = 9.8ARR38 pKa = 11.84ATQQYY43 pKa = 9.28EE44 pKa = 3.89AGNLSKK50 pKa = 10.69SQRR53 pKa = 11.84DD54 pKa = 3.89KK55 pKa = 11.08IRR57 pKa = 11.84ARR59 pKa = 11.84ANKK62 pKa = 9.37VLYY65 pKa = 10.4GG66 pKa = 3.64

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

20944

28

3537

249.3

27.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.477 ± 0.461

1.003 ± 0.185

5.567 ± 0.198

7.066 ± 0.239

3.71 ± 0.167

5.954 ± 0.233

1.681 ± 0.141

7.176 ± 0.239

6.699 ± 0.32

8.79 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.151

5.949 ± 0.224

3.834 ± 0.135

4.311 ± 0.352

4.206 ± 0.183

6.431 ± 0.175

6.255 ± 0.278

6.403 ± 0.2

1.074 ± 0.124

3.796 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski